Bombyx mori glutathione-S-transferase BmGSTe4 gene

A technology of glutathione and transferase, which is applied in the direction of transferase, genetic engineering, plant gene improvement, etc., can solve the problems of increasing the dosage of pesticides, affecting the sustainable development of the economy and human beings, and environmental burdens, so as to inhibit cell apoptosis death effect

Inactive Publication Date: 2013-06-19
SOUTHWEST UNIV
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Problems solved by technology

[0002] Pesticides play a very important role in pest control, but with the use of a large number of pesticides, insect resistance is increasing, forcing people to develop new pesticides or increase the dose of pesticides, which not only restricts economic development, but also brings environmental impacts. A serious burden has come, which affects the sustainable development of the economy and human beings

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  • Bombyx mori glutathione-S-transferase BmGSTe4 gene

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Embodiment Construction

[0017] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0018] one, B m Cloning of the GSTe4 gene

[0019] Based on the predicted coding sequence (CDS) in the silkworm genome, in the silkworm expressed sequence tag (EST) database is B m GSTe4 gene searched for EST evidence, and designed the following specific primers based on CDS and EST sequences: BmGSTe4-F: 5'-ccc aagctt atggtgttcatcctgtac-3' (SEQ ID No. 3); BmGSTe4-R: 5'-atg ccatgg atggtgttcatcctgtac-3' (SEQ ID No.4); the underlined parts are HindIII and NcoI restriction sites respectively.

[002...

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Abstract

The invention discloses a bombyx mori glutathione-S-transferase BmGSTe4 gene with a nucleotide sequence shown in SEQIDNo.1, and the expression product thereof has glutathione-S-transferase (GSTs) activity, because GSTs plays an important role in the formation of pesticide resistance and oxidation resistance of insects, and bombyx mori is a lepidopterous insect-model organism, a biological control pesticide which can specifically kill lepidoptera pests and is harmless to other beneficial insects is expected to be designed through taking BmGSTe4 genes as targets. In addition, in the invention, a situation that BmGSTe4 genes have a function of inhibiting cell apoptosis is found, therefore, the BmGSTe4 genes can be used as potential targets of anti-cancer drug screening.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a silkworm glutathione-S-transferase gene. Background technique [0002] Pesticides play a very important role in pest control, but with the use of a large number of pesticides, insect resistance is increasing, forcing people to develop new pesticides or increase the dose of pesticides, which not only restricts economic development, but also brings environmental impacts. A serious burden has come, which has affected the sustainable development of the economy and human beings. In addition, the resistance of insects is related to the antioxidant capacity of insects. For example, insects may produce some reactive oxygen species in the process of detoxifying and metabolizing organophosphorus pesticides, thereby inducing the oxidative stress response of insects. At the same time, its antioxidant capacity is also increased. How to use today's rapidly developing genetic engineering technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/866C12N7/01C12N9/10C12R1/93
Inventor 潘敏慧鲁成刘佳陈向云余泉友代方银向仲怀
Owner SOUTHWEST UNIV
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