Method for creating tomato cytoplasmic male sterility new germplasm through MSH1 gene editing and application

By editing the tomato MSH1 gene using CRISPR/Cas9 technology, a new germplasm of cytoplasmic male sterility was created, solving the problem of insufficient cytoplasmic male sterility material in tomatoes, achieving a highly efficient and easily maintained sterility trait, and promoting the industrialization of tomatoes.

CN121915048APending Publication Date: 2026-04-24ZHEJIANG UNIV
0 Cites 0 Cited by

Patent Information

Application Number
CN202512044156.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-31
Publication Date
2026-04-24

AI Technical Summary

Technical Problem

The current shortage of cytoplasmic male sterile materials in tomatoes limits the efficiency and purity of tomato hybrid production, making large-scale production difficult.

Method used

Gene editing of the exon region of the tomato MSH1 gene was performed using CRISPR/Cas9 technology. Mitochondrial genome recombination was induced by the plant's own DNA repair mechanism to create new cytoplasmic male sterile germplasm. Sterile materials with the MSH1 gene restored to wild type were obtained through self-pollination and hybridization screening.

Benefits of technology

The creation of non-transgenic cytoplasmic male sterile materials makes it easier to maintain sterility traits, improves the production efficiency and purity of tomato hybrids, promotes the industrialization of tomatoes, and provides important materials for nucleocytoplasmic interaction research.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention belongs to the technical field of crop breeding by a gene editing technology, and particularly relates to a method for creating a novel tomato cytoplasmic male sterility germplasm through gene editing and an application of the novel tomato cytoplasmic male sterility germplasm. In order to solve the problems that existing cytoplasmic male sterile materials in tomatoes are insufficient, production of tomato hybrid seeds is restricted and the like, gene editing is performed on MSH1 through a CRISPR / Cas9 technology, cytoplasmic male sterile single plants are identified in mutant selfing separation progenies and hybridized with wild types twice, and the tomato hybrid seeds are obtained. Finally, a novel non-transgenic tomato cytoplasmic male sterility material with the MSH1 gene recovered to a wild type is obtained, and meanwhile, the horticultural character of the original fertile material is kept unchanged.
Need to check novelty before this filing date? Find Prior Art