Method for identifying resistance against heterodera glycines

A soybean cyst nematode and resistance identification technology is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, and can solve the problems of difficult preservation of breeding materials, large workload of resistance identification, material destruction, etc. To achieve the effect of repeated identification

Active Publication Date: 2011-11-23
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Breeding for disease resistance against soybean cyst nematodes is difficult because of the heavy workload of resistance identification, and the existing identification methods are destructive to materials, and it is difficult to preserve breeding materials identified as resistant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 A method for identifying soybean cyst nematode resistance, comprising the following steps:

[0028] (1) Sowing: Germinate the materials to be identified in vermiculite or sow them in the field according to conventional methods.

[0029] (2) Sampling: When the plant grows to the branching stage, cut off the main stem and the young part of the top of the branch, and keep a newly expanded compound leaf.

[0030] (3) Rooting: Soak the roots of the cut branches in 0.1% ABT rooting powder solution for 15-20 seconds, then put them in clean water, keep the water temperature at 25±1°C, change the clean water every 2 days, after 10 days When the root system grows to about 10cm, it is transplanted into the soil to make it grow.

[0031] (4) Transplanting: Mix loam and sand at a ratio of 2:1, bake at 150°C for 4 hours, put them in plastic cups of φ6 cm×h12 cm, drill holes at the bottom of the cups, put each 60 cups in a 70 cm ×40 cm plastic basket. When the new root...

Embodiment 2

[0034] Embodiment 2 Implementation of the verification test of the identification method described in 1

[0035] (1) Test materials: the international common susceptible host Lee was used as the susceptible material, and the Xingxian Huipi branch (ZDD2315) was used as the resistant material.

[0036] (2) Test method: 20 seeds of each species of the susceptible host Lee and the resistant material Xingxian Huipizhi (ZDD2315) were placed in vermiculite to germinate, and then transplanted into cups filled with sterilized soil. When the plant grows 2 to 3 compound leaves, cut off about 8 cm from the top of each plant. Soak the cut part in 0.1% ABT rooting powder solution for 20 seconds, then put it into clean water, keep the water temperature at about 25°C, change the clean water every 2 days, and transplant it to the soil when the root system grows to about 10cm in about 8 days After surviving, they were inoculated with the cyst nematode suspension at the same time as the origina...

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PUM

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Abstract

The invention relates to a method for identifying resistance against heterodera glycines. The method comprises the following steps: scissoring the tissue of young part of the soybean vegetative part of the susceptible control variety and the variety to be identified to perform inductive rooting culture; after the transplanted seedling survives, inoculating the seedling with heterodera glycines suspension containing 400 eggs and/or second-stage larvae/mL; continuing culture at 20-30 DEG C; when white cyst appears at the seedling root, separating counting the number of root cyst of the susceptible control variety and the variety to be identified; and defining the disease resistance of the material according to the resistance grading standard. The method provided by the invention has the positive and beneficial effects that: as the genotype is not changed when the vegetative body roots, the resistance of the vegetative body can represent the resistance of the parent material; one part of the vegetative body of the material to be identified replaces the entire plant for identification, and the resistive material can be completely stored; and for the population of segregative generation (for example, F2), 3-5 branches on one individual plant can be fetched for identification so as to realize repeated identification of the segregation population.

Description

technical field [0001] The invention relates to the field of identification of crop disease resistance, in particular to a new method for identification of soybean cyst nematode resistance. Background technique [0002] Soybean cyst nematode (SCN) is a major disease of soybean caused by soybean cyst nematode ( Heterodera glycines ) infection is one of the most prevalent and devastating diseases in soybean production in the world. Soybean cyst nematode disease generally causes a loss of 5-10% of soybean yield, and in serious cases, the yield of the plot can be reduced by more than 30%, and even the grains cannot be harvested. Breeding and promoting disease-resistant soybean varieties is the most cost-effective control measure. Breeding for disease resistance against soybean cyst nematodes is difficult because of the heavy workload of resistance identification, and the existing identification methods are destructive to materials, and it is difficult to preserve breeding mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01G1/00
Inventor 卢为国李金英文自翔李海朝李锦锦张辉武永康单志慧
Owner HENAN ACAD OF AGRI SCI
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