The invention discloses a nanometer Ag antimicrobial comprising nanometer Ag particle, a solubilizer, a water-soluble polymer, a plasticizer, a processing modifier, a solvent, a film-forming assistant, essence, pigment, distilled water or deionized water. The invention also disclosed a preparation method of the nanometer Ag antimicrobial. The preparation method comprises the steps of: adding the nanometer Ag particle into the distilled water or the deionized water, adding the solubilizer and the water-soluble polymer, and stirring to prepare a solution A; adding the plasticizer and the processing modifier (acrylate (ACR)) into the solvent, stirring, adding the film-forming assistant, and stirring to prepare a solution B; and adding the solution A into the solution B, adding the essence and the pigment, and stirring to obtain the nanometer Ag antimicrobial. The nanometer Ag antimicrobial disclosed by the invention has the advantages of strong adhesion on objects, good film forming property, good friction resistance and long-lasting antibacterial effect; and the preparation method has the advantages of simpleness and strong operability.