The application belongs to the technical field of
small molecule fluorescent probe, and provides a kind of nonamethylenecyclooctyne dye probe and its application in
disease diagnosis.A kind of nonamethylenecyclooctyne dye is prepared, and the dye can be rapidly covalently combined with
serum albumin in vivo or
in vitro to form
fluorescent protein, which is suitable for targeted imaging of various diseases.Nonamethylenecyclooctyne dye is injected through
tail vein, rapidly combined with
serum albumin in vivo, and can be used for targeted imaging and diagnosis of different degrees of
brain damage in mouse
stroke and targeted imaging of tumor.In addition, the pre-prepared nonamethylenecyclooctyne dye and
human serum albumin complex can also be injected through
tail vein to achieve rapid targeted imaging of tumor.Compared with ICG, NIR-940 can rapidly covalently combine with
serum albumin at 37 DEG C or even
room temperature, overcoming the defect of non-covalent combination of ICG, and being suitable for high-contrast targeted imaging of
disease area.