Leguminous bacterium having potentiated mitrogen fixation ability

Abstract

The invention of this application provides root nodule bacteria transformed with the DNA fragment of SEQ ID No. 1 consisting a catalase gene and whose nitrogen-fixation ability is enhanced with a catalase produced from the catalase gene, a preparation for leguminous crops containing the root nodule bacteria as an active ingredient, and a method of cultivating leguminous crops which comprises contacting seeds of leguminous crops with these. These inventions enable the acceleration of growth of leguminous crops and multiplication of crop yield of beans. Further, the reduction of agricultural cost and the alleviation of environmental pollution are realized by the reduction of nitrogen fertilizers.

Description

technical field [0001] The invention of the present application relates to a rhizobia with enhanced nitrogen fixation ability through the expression of the introduced catalase gene, and a preparation for leguminous crops using the rhizobia as an active ingredient. Background technique [0002] Leguminous crops such as soybeans, adzuki beans, and kidney beans form symbiotic root nodules due to rhizobia infection. Since the rhizobia formed in the root nodules can fix nitrogen in the air, they can be well maintained even in soils with low nitrogen content. grow. [0003] Therefore, when cultivating leguminous crops, in addition to supplying common fertilizers, pre-cultured rhizobia can be applied to the seeds of the crops. [0004] For the purpose of promoting the growth and yield increase of various leguminous crops, the rhizobia strains suitable for each leguminous crop have been searched tirelessly. In addition, attempts have been made to develop transformed rhizobia throu...