Leguminous bacterium having potentiated mitrogen fixation ability

A rhizobia, nitrogen fixation technology for bacteria, microbe-based methods, enzymes, etc.

Inactive Publication Date: 2004-10-13
JAPAN SCI & TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it was not known that catalase activity enhances the nitrogen fixation ability of rhizobia. In addition, it was not known at all that the nitrogen fixation ability of rhizobia was enhanced by introducing the catalase gene.

Method used

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Embodiment

[0023] Examples are given below to further describe the invention of the present application in detail, but the invention of the present application is not limited to the following examples.

[0024] 1. Construction of recombinant vector

[0025] The vktA gene (containing a promoter sequence and a catalase coding region) consisting of a sequence of 1 base sequence was inserted into a vector with a wide host domain (pBBR1MCS-2: Gene 166: 175-176, 1995), and a vktA gene was constructed. Recombinant vector pBBR1MCS-2:vktA.

[0026] 2. Construction of transformed bacteria

[0027] 2.1 Strains used

[0028]Rhizobium leguminosarum mbv. phaseoli 2676 (Phaseolus) and Rhizobium fredii USDA 191 (Soybean fungus) were used as rhizobium bacteria (recipient bacteria) for transformation. Escherichia coli JM109 transformed with the recombinant vector pBBR1MCS-2:vktA was used as a donor bacterium. Escherichia coli E. coli MM294 possessing pRK2013 was used as a helper plasmid possessing bac...

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Abstract

The invention of this application provides root nodule bacteria transformed with the DNA fragment of SEQ ID No. 1 consisting a catalase gene and whose nitrogen-fixation ability is enhanced with a catalase produced from the catalase gene, a preparation for leguminous crops containing the root nodule bacteria as an active ingredient, and a method of cultivating leguminous crops which comprises contacting seeds of leguminous crops with these. These inventions enable the acceleration of growth of leguminous crops and multiplication of crop yield of beans. Further, the reduction of agricultural cost and the alleviation of environmental pollution are realized by the reduction of nitrogen fertilizers.

Description

technical field [0001] The invention of the present application relates to a rhizobia with enhanced nitrogen fixation ability through the expression of the introduced catalase gene, and a preparation for leguminous crops using the rhizobia as an active ingredient. Background technique [0002] Leguminous crops such as soybeans, adzuki beans, and kidney beans form symbiotic root nodules due to rhizobia infection. Since the rhizobia formed in the root nodules can fix nitrogen in the air, they can be well maintained even in soils with low nitrogen content. grow. [0003] Therefore, when cultivating leguminous crops, in addition to supplying common fertilizers, pre-cultured rhizobia can be applied to the seeds of the crops. [0004] For the purpose of promoting the growth and yield increase of various leguminous crops, the rhizobia strains suitable for each leguminous crop have been searched tirelessly. In addition, attempts have been made to develop transformed rhizobia throu...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00C05F11/08C12N1/21C12N5/02C12N9/08C12N15/09C12N15/53C12R1/41
CPCC12N9/0065C05F11/08
Inventor 奥山英登志大和田琢二一濑信敏
Owner JAPAN SCI & TECH CORP
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