2 results about "Catalase Gene" patented technology

The application discloses genetically engineered bacteria for producing alpha-ketoglutaric acid and application. The genetically engineered bacteria are obtained by weakening the expression of alpha-ketoglutaric acid dehydrogenase gene odhA, weakening the expression of glutamate transporter protein gene cg1434, weakening the expression of glutamate synthase key subunit gene gltB, weakening the expression of lactic acid dehydrogenase gene ldh, weakening the expression of glutamate dehydrogenase gene gdh, weakening the expression of serine / threonine protein kinase gene pknG, enhancing the expression of pyruvate carboxylase gene pyc, introducing and expressing glutamate oxidase gene lgox at the site of lactic acid dehydrogenase ldh gene, and enhancing the expression of strong catalase gene katA on the basis of a Corynebacterium glutamicum starting strain. The genetically engineered bacteria can efficiently produce alpha-ketoglutaric acid under the condition of limited ammonia culture, and have a good application prospect.

The invention belongs to the technical field of fish biology, and relates to a knock-in system for stable inheritance and real-time fluorescence monitoring of zebra fish cat gene expression quantity, a construction method and application. Based on an MMEJ repair strategy, the applicant screens out an efficient sgRNA target site in a catalase cat genome, designs an efficient knock-in plasmid containing DgRNA with characteristics preferred by MMEJ repair, and injects a knock-in system into a zebrafish embryo through micro-injection to obtain a fluorescently labeled zebrafish model targeting catalase cat. In the model, the integrity of a cat coding sequence can be kept, the cat gene expression level is traced in vivo, and a technology for analyzing the influence of a compound on cat activity through in-vivo detection of the expression quantity of fluorescent protein is established, so that the method can be used for detecting oxidative stress reaction caused by environmental pollutants and evaluating antioxidant active substances, and has a wide application prospect. The method has important application prospects in the fields of environment detection, cosmetics and medicines.