46 results about "Catalase Gene" patented technology

The invention relates to a novel-originated catalase and the construction of recombinant engineering strains of the catalase. The construction is characterized in that primers are designed according to a catalase gene of bacillus pumilus for the first time, and recombinant expression vectors are constructed; the vectors are converted to genetic engineering strains, i.e., Escherichia coli and Bacillus subtilis for heterologous expression; the recombinant engineering strains are subjected to fermented cultivation through a 5L fermentation tank by using a fermentation culture medium, wherein catalase of recombinant Escherichia coli is mainly subjected to intracellular expression and has the enzyme activity of 6645U / mL, catalase of recombinant B. subtilis 168 has the total enzyme activity of 23,263U / mL and the extracellular enzyme activity of 15,368U / mL, catalase of B. subtilis WB600 has the total enzyme activity of 26,635U / mL and the extracellular enzyme activity of 20,128U / mL, the extracellular synthesis of the catalase is better facilitated by using the B. subtilis WB600 as a host, and thus the B. subtilis WB600 has an important industrial application value. The invention achieves the maximum yield compared with currently-reported methods which are used for producing the catalase by using microbes, so that a practical and effective policy for industrial production of the catalase is provided.

The invention belongs to the field of genetic engineering, and relates to a method for improving the enzyme activity of heterologous expression of L-amino acid deaminase, which uses L-amino acid deaminase (L-amino acid deaminase, LAAD) gene and catalase (Catalase) Gene expression in tandem. The present invention adopts L-amino acid deaminase to be expressed in series with catalase, which increases the enzyme activity per unit cell and increases the amount of cell per unit fermentation volume, thereby having high industrial application value.

The invention relates to a method for detecting the diversity of bacterial catalase in seawater by using degenerate primers, designing a combination of four kinds of degenerate primers based on the conserved region of bacterial catalase, and using polymerase chain reaction to amplify the diversity of bacterial catalase in seawater. Catalase gene fragments, and then use Escherichia coli to construct a library of enzyme gene fragments, use restriction endonucleases to digest the enzyme genes in the library, detect the molecular weight distribution of the fragments by electrophoresis, and determine the types of different electrophoresis bands, and finally Different types of catalase genes were sequenced separately to obtain information on the diversity of catalase genes. This method is an accurate, reliable, rapid, new method using degenerate primers to detect the diversity of bacterial catalase in seawater, and can be used to detect the diversity of bacterial catalase in seawater.

The present invention relates to a novel signal sequence for mass-expression of catalase and a use thereof and, more specifically, to a novel lipase signal sequence; an expression vector comprising the signal sequence and a catalase gene; a recombinant microorganism into which the expression vector is introduced; and a method for mass-producing catalase by culturing the recombinant microorganism. The use of the expression vector comprising the novel signal sequence, according to the present invention, can induce mass expression and secretion of particular catalase and other target proteins, and thus the expression vector is very useful in the stable mass production of the particular catalase and other target proteins.