9 results about "Catalase Gene" patented technology

The application discloses genetically engineered bacteria for producing alpha-ketoglutaric acid and application. The genetically engineered bacteria are obtained by weakening the expression of alpha-ketoglutaric acid dehydrogenase gene odhA, weakening the expression of glutamate transporter protein gene cg1434, weakening the expression of glutamate synthase key subunit gene gltB, weakening the expression of lactic acid dehydrogenase gene ldh, weakening the expression of glutamate dehydrogenase gene gdh, weakening the expression of serine / threonine protein kinase gene pknG, enhancing the expression of pyruvate carboxylase gene pyc, introducing and expressing glutamate oxidase gene lgox at the site of lactic acid dehydrogenase ldh gene, and enhancing the expression of strong catalase gene katA on the basis of a Corynebacterium glutamicum starting strain. The genetically engineered bacteria can efficiently produce alpha-ketoglutaric acid under the condition of limited ammonia culture, and have a good application prospect.

The invention belongs to the field of prevention and control of tomato leaf miners, and discloses application of CQD-dsRNA in prevention and control of tomato leaf miners. The method comprises the following steps: screening catalase possibly existing in the oral secretion of the tomato leaf miner, designing a dsRNA fragment and a primer of a targeted catalase gene, carrying out in-vitro transcription to synthesize corresponding dsRNA, carrying the dsRNA by using a carbon quantum dot CQD, and delivering the dsRNA to the body of a tomato leaf miner larva. It is found that the silence efficiency, the survival rate and the pupation number of the tomato leaf miner catalase gene are remarkably reduced compared with those of pure dsRNA, a target is provided for prevention and control of tomato leaf miner RNAi, and a reference is provided for RNAi prevention and control of tomato leaf miner in the mode that CQD carries catalase gene dsRNA.

The present invention relates to host cells comprising genes of the mevalonate, lycopene and α-ionone pathways and the hydroperoxide reductase or catalase genes. The present invention also relates to methods of producing apocarotenoids as well as kits for producing apocarotenoids comprising the host cells.

The invention provides a deinococcus radiodans and saussurea involucrata co-fermentation product as well as a preparation method and application thereof, and belongs to the technical field of cosmetic raw material development. According to the invention, deinococcus radiodurans and saussurea involucrata are subjected to co-fermentation, such that a deinococcus radiodurans and saussurea involucrata co-fermentation product is obtained. An anti-oxidation experiment shows that the expression of catalase genes and glutathione peroxidase genes after UVB irradiation is remarkably reduced, and the expression of the catalase genes and the glutathione peroxidase genes is remarkably increased after the co-fermentation product of the deinococcus radiodurans and the saussurea involucrata provided by the invention is added, so that the co-fermentation product has an obvious anti-oxidation effect; a cDNA repair experiment shows that the content of pyrimidine dimer is increased after UVB irradiation, the content of pyrimidine dimer is remarkably reduced after the deinococcus radiodurans and saussurea involucrata co-fermentation product is added, and it is proved again that the product has the excellent anti-oxidation effect, and the application prospect is wide. The deinococcus radiodurans and saussurea involucrata co-fermentation product can be used as an anti-ultraviolet radiation cosmetic raw material, and has a very good application prospect.

The invention discloses a recombinant expression plasmid of a neurospora crassa catalase gene, a recombinant trichoderma reesei engineering bacterium as well as a construction method and application of the recombinant expression plasmid and the recombinant trichoderma reesei engineering bacterium. The cdna1 promoter and the cbh1 signal peptide, the catalase gene cat-3, the cbh2 gene terminator and the 2000bp sequence at the downstream of the cel3c gene needing to be replaced are sequentially contained in the sequence of the cdna1 promoter and the cbh1 signal peptide, and the catalase gene cat-3 and the cbh2 gene terminator are sequentially contained in the sequence of the 2000bp at the upstream of the cel3c gene needing to be replaced. The recombinant Trichoderma reesei engineering bacteria are obtained by transforming recombinant expression plasmids into Trichoderma reesei and screening. According to the invention, the neurospora crassa catalase gene cat-3 is heterologously expressed at the trichoderma reesei cel3c site, so that the beta-glucosidase is increased, and the defects existing when the trichoderma reesei is fermented to produce the cellulase are overcome; and the expression strain has good stability and protein secretion ability.

The invention discloses a citrus catalase gene CAT plant expression vector and application thereof, and belongs to the technical field of citrus, the citrus catalase gene CAT plant expression vector comprises a citrus catalase CAT gene and a plant expression vector, the citrus catalase CAT gene is ESR48603; the citrus CAT gene is cloned from Krimann tangerine, subcellular localization, expression analysis and transgenic function identification are carried out, a foundation is laid for defining physiological functions and action mechanisms of the citrus CAT gene, a theoretical basis and part of candidate gene resources are provided for citrus stress resistance molecular breeding, and the citrus CAT gene has reference significance for citrus stress resistance research.

The invention provides a method for biologically synthesizing hydroxytyrosol from escherichia coli by using levodopa. The method comprises the following steps: by taking escherichia coli as a chassis host bacterium, constructing a way for biologically synthesizing hydroxytyrosol by taking levodopa as a substrate through overexpression of an alcohol reductase gene yqhD, a dopa decarboxylase gene dodc and a tyramine oxidase gene tyo; the method comprises the following steps: knocking out a phenylacetaldehyde oxidase gene feaB and a 4, 5-DOPA external diol dioxygenase gene ygiD, so as to reduce the generation of by-products; nusA tag protein is fused at the N end of tyramine oxidase gene tyo, and catalase gene KatG is overexpressed, so that a synthesis path of hydroxytyrosol is optimized; according to the present invention, the expression of the glucose-6-phosphate isomerase gene pgi is inhibited so as to adjust the carbon flux of the glycolysis pathway and the pentose phosphate pathway, such that the hydroxytyrosol biosynthesis pathway is reinforced, and the efficient hydroxytyrosol biosynthesis is finally achieved by using the levodopa; the invention provides a new scheme for biosynthesis of hydroxytyrosol.

The present invention discloses a catalase gene and its application. By means of genetic engineering, the present invention constructs an expression cassette of catalase and introduces it into a recombinant expression host bacterium, achieving the high-efficiency secretory expression of catalase and obtaining a high-yield catalase recombinant strain. Compared with the catalase gene before optimization, its catalase activity is significantly improved, reaching 8907 U / ml. Using the fermentation process of the present invention, in a 7L fermenter for fermentation culture, the catalase activity can reach 368900 U / mL. The obtained catalase has an optimal temperature of 40°C, with a relative enzyme activity greater than 80% at pH 5 - pH 9, and can still maintain 70% of its enzyme activity after 3 minutes at 75°C. The enzyme activity is relatively stable and has good heat resistance, and can be widely used in food, textile, medical, industrial, and paper-making industries.

The invention belongs to the technical field of fish biology, and relates to a knock-in system for stable inheritance and real-time fluorescence monitoring of zebra fish cat gene expression quantity, a construction method and application. Based on an MMEJ repair strategy, the applicant screens out an efficient sgRNA target site in a catalase cat genome, designs an efficient knock-in plasmid containing DgRNA with characteristics preferred by MMEJ repair, and injects a knock-in system into a zebrafish embryo through micro-injection to obtain a fluorescently labeled zebrafish model targeting catalase cat. In the model, the integrity of a cat coding sequence can be kept, the cat gene expression level is traced in vivo, and a technology for analyzing the influence of a compound on cat activity through in-vivo detection of the expression quantity of fluorescent protein is established, so that the method can be used for detecting oxidative stress reaction caused by environmental pollutants and evaluating antioxidant active substances, and has a wide application prospect. The method has important application prospects in the fields of environment detection, cosmetics and medicines.