Catalase with high enzyme activity, gene, recombinant strain with high catalase yield and application

A catalase and recombinant strain technology, applied in the field of genetic engineering, can solve the problems of enzyme separation, complex purification, high yield and inconvenient extraction, and achieve the effect of efficient production

Active Publication Date: 2021-03-19
GUANGDONG VTR BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Animal red blood cells, liver, and bacterial catalase exist in the cytoplasm, and the cells must be broken to extract catalase, so the separation and purification of the enzyme are more complicated
Although the thermal and alkaline stability of bacterial catalase can vary with different sources, because it is an intracellular enzyme, it is inconvenient to achieve high yield and extraction

Method used

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  • Catalase with high enzyme activity, gene, recombinant strain with high catalase yield and application
  • Catalase with high enzyme activity, gene, recombinant strain with high catalase yield and application
  • Catalase with high enzyme activity, gene, recombinant strain with high catalase yield and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, fungus Rasamsonia sp. catalase CatR gene cloning

[0067] 1. Fungus Rasamsonia sp. catalase CatR gene synthesis.

[0068] Soil samples of the fungus Rasamsonia sp. strain from Zhuhai, China were passed through the dilution plate, and a small amount of catalase activity was detected from the screening plate. After the morphological characteristics and ITS rDNA sequence, the strain was identified as Rasamsonia sp. Using primers CatR-F, CatR-R to amplify the catalase gene fragment. Its nucleotide sequence is SEQ ID NO: 2, and its coded amino acid sequence is SEQ ID NO: 1.

[0069] PCR primers and reaction conditions are as follows:

[0070] CatR-F(5'-3'): ATGCGCGCTGTGCAACTTCTGCCCAGC

[0071] CatR-R (5'-3'): TCATTACTCATCCAGCGCAAACCGGTCGAGGAAC

[0072] Reaction conditions:

[0073] Table 1

[0074]

[0075] PCR amplification was carried out with the above primers to obtain a DNA band with a size of about 2.2 kb, and the target fragment was recovered. ...

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Abstract

The invention relates to the field of gene engineering, in particular to catalase with high enzyme activity, a gene, a recombinant strain with high catalase yield and application. The amino acid sequence of catalase catR is as shown in SEQ ID NO:1. The catalase with high enzyme activity and the gene of the catalase are provided, and the recombinant strain for efficiently expressing the catalase isobtained. The recombinant strain produced by fermentation can efficiently produce the catalase. The catalase disclosed by the invention is widely applied to production of sodium gluconate.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a catalase with high enzyme activity, a gene, a recombinant strain and an application of a high-production catalase. Background technique [0002] Catalase (Hydrogen peroxide oxidoreductase, catalase EC 1.11.1.6.) is a class of enzymes that use hydrogen peroxide as a specific substrate, and finally degrade it into water and oxygen by catalyzing the transfer of a pair of electrons. The enzyme is one of the key enzymes in the biological defense system established in the process of biological evolution, and has important applications in food, medicine, textile, papermaking, environmental protection and other industries. [0003] Catalase (CAT) is a new type of enzyme preparation, which is widely distributed in the biological world. CAT is the natural enemy of oxygen free radicals, it can eliminate the harmful substances produced by the organism in the metabolic process, and conti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08C12N15/53C12N15/80C12N1/15C12R1/685C12R1/645
CPCC12N9/0065C12Y111/01006C12N15/80
Inventor 李阳源黄江边叶雨江民华刘金山王勇王水生唐鹏陈琼银陈丽芝黄文德李鹏
Owner GUANGDONG VTR BIO TECH
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