The invention discloses a method by using a co-expression recombinant
bacteria strain to convert and product alpha-ketoglutarate, and belongs to the technical field of
biology. The method is characterized in that novel L-glutamic oxidase is screened from streptomycete and is induced to express and purify in
escherichia coli, and the enzymatic property is studied; when the pH (potential of
hydrogen) value is 5.5 to 7.0, the
enzyme has higher activity, the most suitable
reaction temperature is 30 to 45 DEG C, Vmax is 100 to 150U / mg, and Km is 8 to 10mM; the original
gene sequence of the L-glutamic oxidase is subject to
codon optimization, and the
plasmid co-expression with a
catalase gene from the
escherichia coli is performed, so as to construct the co-expression recombinant
bacteria strain; the recombinant
bacteria strain is used as a whole-
cell catalyst to convert the L-
glutamic acid (salt), the output of alpha-ketoglutarate reaches 76.08g / L after reacting for 9h, and the
molar conversion rate is 96.8%. The method has the advantages that the problems of complicated production steps, low yield,
pollution to environment and the like in the alpha-ketoglutarate production process aresolved, the high-yield and one-step type production of the alpha-ketoglutarate is realized, and the industrial application value is higher.