Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method

A technology for dry and rapid determination of alanine aminotransferase, applied in biochemical equipment and methods, biological testing, microbial determination/inspection, etc., can solve the problems of quantification of difficult detection results, and achieve the effect of shortening the process

Active Publication Date: 2007-06-27
WUXI SHENRUI BIO PHARMA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The main problem with this reagent strip is that it is difficult to accurately quantify the test results.

Method used

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  • Dry chemical quick detecting reagent strip for glutamic-pyruvic transaminase and its producing method

Examples

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Embodiment Construction

[0014] As shown in the figure: a carrier film adsorbed with reaction substances is adhered to a certain hardness of PVC hard slats 6, and the reaction substances include α-ketoglutaric acid, L-alanine, pyruvate oxidase and hydrogen peroxide Enzymes, a layer of carrier film 5 adsorbed with α-ketoglutarate is attached to the strip 6, and a layer of pyruvate oxidase is respectively attached to the carrier film 5 with adsorbed α-ketoglutarate Carrier membrane 1 with catalase and carrier membrane 4 adsorbed with L-alanine, and a mesh pre-filter device for equalizing samples is attached to the carrier membrane 4 with adsorbed L-alanine 3. Adhere a layer of transparent protective film 2 on the outermost carrier film to protect the entire reaction device. The carrier membrane 1 that has adsorbed pyruvate oxidase and catalase and the carrier membrane 4 that has adsorbed L-alanine are separated from each other. The reagent strip also includes a corresponding color card. The carrier me...

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Abstract

Purpose of the invention is to provide a reagent bar capable of half quantitative/ quantitative detecting whether content of glutamic-pyruvic transaminase (GPT) inside human body is normal or not rapidly, and relevant fabricating method. The reagent bar includes PVC hard strip for supporting reaction. The hard strip possesses carrier film of adsorbing L-alanine, pyruvate oxidase, and alpha-ketoglutarate. Belonging to fast test in micro scale, the disclosed product is one-off consumption material for testing. Range for detecting GPT is 0-1000U/L.

Description

technical field [0001] The invention relates to a rapid detection reagent strip and a manufacturing method thereof, in particular to a reagent strip and a manufacturing method for detecting alanine aminotransferase. Background technique [0002] Reagent strips are mainly used for rapid qualitative detection of GPT content in the human body, to understand the current degree of liver damage in the human body and to judge the effect of treatment, and can also be used for routine examination of healthy people to detect liver function. At present, the reagent strip is in the mature stage of development, and the relevant detection can be directly carried out by using the reagent strip and the matching color card. The main problem with this reagent strip is that it is difficult to accurately quantify the test results. Contents of the invention [0003] The purpose of the present invention is to provide a rapid assay reagent strip for alanine aminotransferase dry chemical method ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/25C12Q1/52
Inventor 盛青松
Owner WUXI SHENRUI BIO PHARMA
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