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Kit for diagnosing diseases in system of liver and gall

A disease diagnosis and kit technology, applied in the field of biological preparations, can solve the problems of not reaching the maximum reaction speed, poor repeatability of low-value samples, unbalanced enzyme reactions, etc., to achieve easy popularization and application, good stability and good accuracy and the effect of sensitivity

Inactive Publication Date: 2007-07-25
王贤理
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the storage time of NADH in the reagent prolongs, NADH will be converted into NAD, which will affect the whole reaction, because the content of bile acid in serum is very low, and the concentration of NADH will change, which will make the enzyme reaction unbalanced and fail to reach the maximum. The reaction speed directly affects the accuracy of the results, and the repeatability of low-value samples is very poor

Method used

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  • Kit for diagnosing diseases in system of liver and gall

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Reagent 1:

[0057] Buffer Glycine 7.5g / L

[0058] Thio-oxidized coenzyme Thio-NAD 0.81g / L

[0059] Preservative NaN 3 0.2g / L

[0060] Surfactant Tween-20 3ml / L

[0061] Anti-jamming agent sodium oxamate 1.0g / L

[0062] Anti-jamming agent dermaran G50 0.5g / L

[0063] Anti-jamming agent BPS 0.5g / L

[0064] Reagent 2:

[0065] Buffer 3-(cyclohexylamine)-2-hydroxypropanesulfonic acid 15.0g / L

[0066] Surfactant Tween-20 1ml / L

[0067] Formic acid 0.8g / L

[0068] Formate dehydrogenase 5ku / L

[0069] 3αHSD 12ku / L

[0070] Reduced coenzyme NADH 6.1g / L

[0071] Preservative NaN 3 0.2g / L

[0072] Complexing agent EDTA.2Na 0.75g / L

[0073] Polymer accelerator PEG-6000 2.5g / L

[0074] Stabilizer Trehalose 10g / L

Embodiment 2

[0076] Reagent 1:

[0077] Buffer Glycine 0.8g / L

[0078] Thio-oxidized coenzyme Thio-NAD 1.0g / L

[0079] Preservative NaN 3 1.0g / L

[0080] Surfactant Tween-20 5.0ml / L

[0081] Anti-jamming agent sodium oxamate 25g / L

[0082] Anti-jamming agent dermaran G50 8.5g / L

[0083] Anti-jamming agent BPS 1.8g / L

[0084] Reagent 2:

[0085] Buffer 3-(cyclohexylamine)-2-hydroxypropanesulfonic acid 5.0g / L

[0086] Surfactant Tween-20 5.0ml / L

[0087] Formic acid 10.0g / L

[0088] Formate dehydrogenase 10ku / L

[0089] 3α-HSD 14ku / L

[0090] Reduced coenzyme NADH 5.0g / L

[0091] Preservative NaN 3 1.5g / L

[0092] Complexing agent EDTA.2Na 0.5g / L

[0093] Polymer accelerator PEG-6000 1.0g / L

[0094] Stabilizer Trehalose 5.0g / L

Embodiment 3

[0096] Reagent 1:

[0097] Buffer Glycine 20.0g / L

[0098] Thio-oxidized coenzyme Thio-NAD 1.2g / L

[0099] Preservative NaN 3 1.5g / L

[0100] Surfactant Tween-20 1.0ml / L

[0101] Anti-jamming agent sodium oxamate 50g / L

[0102] Anti-jamming agent dermaran G50 10.0g / L

[0103] Anti-jamming agent BPS 2.0g / L

[0104] Reagent 2:

[0105] Buffer 3-(cyclohexylamine)-2-hydroxypropanesulfonic acid 30.0g / L

[0106] Surfactant Tween-20 1.5ml / L

[0107] D-glucose 7.0g / L

[0108] Glucose-6-phosphate dehydrogenase 3.0ku / L

[0109] 3αHSD 13ku / L

[0110] Reduced coenzyme NADH 10.0g / L

[0111] Preservative NaN 3 0.5g / L

[0112] Complexing agent EDTA.2Na 1.0g / L

[0113] Polymer accelerator PEG-6000 5.0g / L

[0114] Stabilizer Trehalose 6.5g / L

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Abstract

This invention relates to a test kit for diagnosing hepatic and biliary diseases. The test kit has such advantages as high stability, high accuracy and high sensitivity. The test kit comprises two reagents. Reagent 1 comprises oxidized thio coenzyme, buffer solution, preservative, and anti-interference agent. Reagent 2 comprises 3alpha-HSD, reduced coenzyme, buffer solution, preservative, complexing agent, polymer accelerator, stabilizer, and one of formate dehydrogenase-formic acid-NAD, glutamate dehydrogenase-glutamic acid-oxidized nicotinamide coenzyme, glucose-6-phosphate dehydrogenase-glucose-oxidized nicotinamide coenzyme, glucose dehydrogenase-xylose-oxidized nicotinamide coenzyme, lactate dehydrogenase-alpha-ketoglutarate-oxidized nicotinamide coenzyme, and glycerol dehydrogenase-ethylene glycol-oxidized nicotinamide coenzyme.

Description

technical field [0001] The invention relates to a reagent for measuring serum components, in particular to a reagent for measuring serum bile acid, and belongs to the technical field of biological preparations. Background technique [0002] Medical research shows that the production and metabolism of bile acids are closely related to the liver, and the level of total bile acids in serum is an important indicator reflecting liver parenchyma damage, especially acute hepatitis, cirrhosis, and toxic hepatitis. Variety. However, its concentration in serum is very low (micromolar level), and it is difficult to detect. In the past, it was not practical due to the complexity of detection methods and the requirement of special equipment, and it was difficult to be used for automatic analysis, which limited its application in daily testing. [0003] In the past, the analysis of serum bile acids mainly used high-performance liquid chromatography (HPLC), HPLC-UV spectrometry, gas chrom...

Claims

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Application Information

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IPC IPC(8): C12Q1/32G01N33/96
Inventor 王贤理蒙凯蔡其浩
Owner 王贤理
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