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Multilayer-film dry chemical reagent strip for measuring glutamic-pyruvic transaminase by using enzyme coupled continuous monitoring assay

A technology of alanine aminotransferase and reagent strips, which is applied in the field of determination of alanine aminotransferase, can solve problems such as function and speed limitation, and achieve the effects of high recognition, simple and convenient operation, and improved stability

Inactive Publication Date: 2017-01-11
GETEIN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When measuring a single alanine aminotransferase item, such as mobile blood collection vehicles, its function and speed will be limited

Method used

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  • Multilayer-film dry chemical reagent strip for measuring glutamic-pyruvic transaminase by using enzyme coupled continuous monitoring assay
  • Multilayer-film dry chemical reagent strip for measuring glutamic-pyruvic transaminase by using enzyme coupled continuous monitoring assay
  • Multilayer-film dry chemical reagent strip for measuring glutamic-pyruvic transaminase by using enzyme coupled continuous monitoring assay

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Embodiment Construction

[0018] exist figure 2 On the lower support layer 2, overlap the diffusion membrane 3, the fast glass fiber filter membrane 4, the mixed fiber membrane 5, and the LP reaction membrane 6 in order from top to bottom, and then press the upper support layer 1 to the lower support layer 2 to fix, get as figure 1 reagent strips.

[0019] The size of each layer of membrane is controlled at about 6×6mm. After pressing and fixing, ensure that the sample injection hole and test hole correspond to the center of the membrane, and there must be no deviation to prevent sample leakage and affect the test results.

[0020] The present invention carries out the detection method of ALT in the blood sample as follows:

[0021] 1. Adding samples: drop liquid samples or processed liquid samples into the sample holes of the reagent strip of the present invention;

[0022] 2. Chromatography osmosis reaction: Stand still for a few minutes to wait for the completion of the chromatographic osmosis p...

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Abstract

The invention discloses a multilayer-film dry chemical reagent strip for measuring glutamic-pyruvic transaminase by using enzyme coupled continuous monitoring assay. The reagent strip comprises four layers of film, namely diffusion film, blood filter film (in two layers) and bottom reaction film and is characterized in that corresponding enzyme reactants are adsorbed to the reaction film, pyruvic acid is generated from L-alanine and sodium Alpha-ketoglutarate under the catalysis of glutamic-pyruvic transaminase, the pyruvic acid acts with reduced coenzyme I(NADH) under the catalysis of lactic dehydrogenase (LDH) to generate NAD+, absorbance at 340 nm is decreased, and the amplitude of decrease is associated with the content of the glutamic-pyruvic transaminase. The reagent strip is applicable to the quick measurement of the activity of glutamic-pyruvic transaminase in human whole blood and serum or plasma, is simple and easy to operate, and is easy to clinically apply and popularize.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection, in particular to a method for measuring alanine aminotransferase. Background technique [0002] Alanine aminotransferase (ALT), also known as alanine aminotransferase, catalyzes the transfer of amino groups between L-alanine and L-glutamic acid. ALT widely exists in various tissues, organs, muscles, and bones of the human body, and is most abundant in the cytoplasm of liver cells. It is a specific enzyme of the liver and is recommended by the World Health Organization as the most sensitive detection index of liver damage. The clinical significance of ALT lies in the diagnosis and analysis of a series of viral hepatitis such as acute hepatitis B, chronic hepatitis, HBV carriers, severe hepatitis, liver cirrhosis, and liver cancer. The increase of ALT only indicates that the liver may be damaged. [0003] There are many detection methods for ALT. The reagents for clinical deter...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31G01N2021/3185
Inventor 童鎏任欣桐苏恩本
Owner GETEIN BIOTECH
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