50 results about "Ceruloplasmin" patented technology

Metallo-proteins including but not limited to lactoferrin (LF), transferrin (TF) and ovotransferrin (OTF) (all members of transferrin family), ceruloplasmin (CP) and metallo-thionein (MT) were found to stabilize and enhance the bio-functional activity of tocotrienol (T3), T3 mixtures or derivates. The synergism between MP and T3 also promote the intestinal transfer and the ultimate bio-availability of T3 and T3-derivatives for physiological functions. Such functional synergism includes hypocholesterolemic, anti-thrombotic, antioxidant, anti-athermogenic, anti-inflammatory and immuno-regulatory activities of T3 agents. These T3 compositions are useful as pharmaceuticals, in cosmetics, in foods and as nutritional supplements.

The invention discloses a human adipose-derived stem cell serum-free basic medium. The medium uses a serum substitute, and is composed of a high glucose type DMEM basic medium, human serum albumin, transferrin, taurine, reduced glutathione, ceruloplasmin, L-ascorbic acid-2-sulfate, alpha-tocopherol succinate, linoleic acid, alpha-ketoglutarate and selenium. The serum-free basic medium can exempt potential threats caused by animal serum in conventional serum-containing mediums to human health, and the adipose-derived stem cells cultured by the medium is more suitable for clinical application.

The invention discloses a human adipose-derived stem cell serum-free basic medium. The medium uses a serum substitute, and is composed of a high glucose type DMEM basic medium, human serum albumin, transferrin, taurine, reduced glutathione, ceruloplasmin, L-ascorbic acid-2-sulfate, alpha-tocopherol succinate, linoleic acid, alpha-ketoglutarate and selenium. The serum-free basic medium can exempt potential threats caused by animal serum in conventional serum-containing mediums to human health, and the adipose-derived stem cells cultured by the medium is more suitable for clinical application.

A neuroprotective composition for protecting neuronal cells against oxidative stress and methods for using and preparing the same. More particularly, the neuroprotective composition of the invention comprises a therapeutically effective amount of ceruloplasmin or a functional derivative thereof. The neuroprotective composition is characterized in that it protects neuronal cells from reactive oxygen species such as .O2- and .OH. In a preferred embodiment, the neuroprotective composition further comprises an antioxidant consisting of catalase or of an amphiphilic physiological antioxidative solution comprising a mixture of pyruvate, antioxidant, and lipid(s) such as fatty acids. The neuroprotective composition could be used for the treatment of brain trauma, brain or cerebrovascular ischemia, neurodegenerative diseases, poisoning of neuronal cells, the diminution of drugs side effects and for preservation of neuronal grafts.

Dithiocarbamate, particularly tetraethylthiuram disulfide, and thiocarbamate anions thereof, strongly inhibit the growth of cancer cells of a variety of cell types. Such inhibitory effect is enhanced by heavy metal ions such as copper ions, cytokines and ceruloplasmin. A method is presented for using tetraethylthiuram disulfide to reduce tumor growth, and to potentiate the effect of other anticancer agents.

Methods for the detection of active lupus nephritis (LN) and worsening renal disease activity and / or active LN in patients diagnosed with systemic lupus erythematosus, using a panel of biomarkers including transferrin (Tf), ceruloplasmin (Cp), alpha-1-acid glycoprotein (AGP1), lipocalin-like prostaglandin D synthetase (L-PGDS), and urinary neutrophil gelatinase associated lipocalin (UNGAL).

The present invention relates to a method for measuring a holoceruloplasmin concentration, and more particularly, to a method for measuring a holoceruloplasmin in a blood spot based on a standard concentration curve obtained through an enzyme-linked immunosorbent assay (ELISA) or a dissociation-enhanced time-resolved fluoroimmunoassay using a holoceruloplasmin-specific polyclonal antibody and a holoceruloplasmin-specific monoclonal antibody.

The invention provides a composition for preventing pneumoconiosis and a preparation method thereof, wherein the composition is prepared from cordyceps militaris, frosted mulberry leaves, tea polyphenols, gingkgo, fructus monordicae extracts, barbary wolfberry fruits, peach kernels, apricot kernels, and malto dextrin, and the preparation method comprises the steps of: (1) soaking the gingkgo, barbary wolfberry fruits, frosted mulberry leaves, peach kernels and apricot kernels in water; (2) heating the soaked materials to be boiling, leaching, then carrying out flocculation precipitation on leach liquor, and concentrating a supernatant fluid into a thick paste; (3) continuously adding water into the rest materials, heating and leaching; (4)mixing and concentrating the two-time extracts into a thick paste, carrying out vacuum drying and then crushing; (5) sieving the extract, and fully mixing with the cordyceps militaris nano powder, malto dextrin, tea polyphenols and fructus monordicae extracts; and (6) fully mixing the extract with the mixture to be uniform, mixing with alcohol, and palletizing. The composition has an obvious free radical scavenging action, reduces ceruloplasmin, obviously restrains pneumoconiosis fibrosis, and greatly improves the respiratory symptoms of patients with pneumoconiosis.

The present invention relates to a method for measuring the concentration of whole plasma ceruloplasmin, in particular to the use of whole plasma ceruloplasmin-specific polyclonal antibody and whole plasma ceruloplasmin-specific monoclonal antibody, by using enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay method) Immunosorbent assay; ELISA) or dissociation-enhanced time-resolved fluorescent immunoassay (dissociation-enhanced time-resolved fluoroimmunoassy) to obtain the standard concentration curve, the method of measuring the concentration of whole plasma ceruloplasmin.

The present invention relates to the use of ceruloplasmin (and / or derivatives thereof) for treating neurodegenerative related conditions. More particularly, the present invention pertains to the use of ceruloplasmin in pharmaceutical neurotrophic compositions, to its use as a carrier for delivering therapeutic agent(s) to tissues of the nervous systems and to its use in methods for culturing neuronal cells in vitro. The present invention is useful for maintaining or stimulating the regeneration of neuronal cells, particularly for the treatment of injuries to the brain, the spinal cord and other central nervous system tissues.

The present invention disclosed a method to detect pregnancy and identify pseudopregnancy, pregnancy and pregnancy loss using ceruloplasmin as a non-invasive marker in animal urine samples. More specifically, this invention provide provides a method to use a non-invasive marker of inflammation, the acute phase protein ceruloplasmin, in urine samples to detect pregnancy and to distinguish between pregnancy and pseudopregnancy in mammals. The steps of this method include: collecting fresh urine sample from a mammal, adding a ceruloplasmin substrate to the urine sample, measuring oxidase activity of said ceruloplasmin through a sequential reading of absorbance of a photometric product using a spectrophotometer to determine the concentration of ceruloplasmin in the at least one urine sample.

The invention relates to a protein marker of focal segmental glomerulosclerosis, and more specifically relates to an application of a connecting part selectively connected to one or more proteins in preparing a diagnostic reagent in a development process of human focal segmental glomerulosclerosis. The one or more proteins are selected from [alpha]1 antiprotease, alpha albumin, serum albumin, beta-2-microglobulin, ceruloplasmin, fetuin-B, kininogen, serum transferrin, carboxylesterase, immune globulin gamma-2A chain C region, plasminogen, lysosomal acid phosphatase, protein YIPF3, zinc [alpha]2 glycoprotein, alpha-2-HS-glycoprotein, protein AMBP or T cell immune globulin. Specifically, the invention relates to the application of a urine protein potential marker of human focal segmental glomerulosclerosis obtained by a rat model and mass spectrometry.

The invention relates to the field of medicine, in particular to a preparation process of human immunoglobulin for intravenous injection. Octanoic acid is used for replacing ethanol as a precipitatingagent, so that acidic protein in blood plasma generates precipitates; IgG molecules with high isoelectric points are reserved on supernate; only through one-step precipitation reaction, the materialscan be transferred to a chromatography working procedure; caprylate is used; lipid envelope viruses are inactivated under the mild condition in short time; even partial non-lipid envelope viruses canbe inactivated; the action time is short; the effect is good; the bioactivity of the immunoglobulin cannot be easily influenced; the product safety is improved; the quality and the yield of the product are ensured; a two-step chromatography working procedure is used; most partial polymers and other impure protein are removed; the pyrogen is reduced; chromoprotein such as ceruloplasmin can be adsorbed; the product appearance is effectively improved; one-step precipitation reaction is used; the loss is reduced; the product yield is improved; the preparation process of human immunoglobulin for intravenous injection has the advantages of high product yield, high purity and high safety.

Provided herein are methods for the diagnosis of obstructive airways diseases such as asthma and chronic obstructive pulmonary disease, and the discrimination between such diseases based on the expression profiles of biomarker proteins and combinations of biomarker proteins. In particular embodiments the biomarker proteins are selected from ceruloplasmin, haptoglobin, hemopexin, -2-macroglobulin, prothrombin, immunoglobulin A and complement factor H.

The invention discloses an oocyte in-vitro maturation culture solution additive. The in-vitro maturation culture solution additive contains ceruloplasmin. Therefore, a first polar body excretion rateof oocytes can be significantly increased, the ROS content of the oocytes can be significantly reduced, and the embryonic blastocyst rate after parthenogenetic activation can be significantly increased, so that the maturation rate and quality of the oocytes can be greatly improved.

The invention discloses a preparation method of a ceruloplasmin detection kit, which comprises the following steps: (1) preparing a PBS buffer solution according to component contents of a reagent R1,adjusting the pH value to obtain an R1 buffer solution, and dissolving NaCl, NaN3, trehalose, triton and PEG-8000 in the R1 buffer solution to obtain the reagent R1; and (2) preparing a PBS buffer solution according to component contents of a reagent R2, adjusting the pH value to obtain an R2 buffer solution, dissolving NaCl, NaN3, BSA and Arabic gum in the R2 buffer solution to obtain an R2 dispersion liquid, preparing a latex-coated ceruloplasmin antibody, and dissolving the latex coated ceruloplasmin antibody by the R2 dispersion liquid to obtain a reagent R2. The preparation method provided by the invention has the following advantages: (1) the operation is simple and rapid; (2) the kit has high sensitivity and accuracy; (3) latex microspheres with different particle sizes are adoptedto improve the sensitivity and the linear range of the kit; (4) the kit has good stability and strong specificity; and (5) the kit is suitable for fully-automatic testing.

The invention relates to a preparation method of a yolk antibody IgY of high-abundance protein in anti-human serum / blood plasma and application thereof; and eggs of a hen immunized by high-abundance protein are used as a source for extracting antibody. The preparation method comprises the steps of: detecting the serum potency of the immunized hen through an indirect ELISA method, obtaining an eggcontaining antibody with high potency after the potency meets the standard; acid hydrolyzing, freeze thawing and filtering the egg yolk liquid, and depositing by using ammonium sulphate; and further purifying through DEAE ion exchange layer chromatograph to obtain the yolk antibody IgY. The high-abundance protein of human serum / blood plasma is a high-abundance protein with albumin, IgG, transferrin, C3 alexin, IgA, IgM, trypsin, globin, lipoprotein, F factor, C9 alexin, C4 alexin, ceruloplasmin, apolipoprotein B, apolipoprotein A-1, alpha-1-acid glycoprotein, H factor, alexin factor B, prealbumin and C1q alexin, and the like, and the concentration of which are higher than 10 mug / ml.

The invention discloses a ceruloplasmin detection kit, and belongs to the technical field of clinic in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2 and a calibration sample. By adding a certain amount of a silicon-dioxide-coated magnetic nano-particle into the reagent R1 and adding a certain amount of bovine serum albumin and Kathon-CG into the reagent R2, the kit stability and analysis sensitivity are effectively improved, the linearity scope is relatively good, the reagent accurate is high, and further popularization usage of the kit in the market is facilitated.

The invention belongs to the technical field of feed preparation, and particularly relates to a preparation method of protein feeds. The preparation method disclosed by the invention comprises the following steps of extracting a compound amino acid concentrate from monosodium glutamate wastewater and then performing chelation on trace elements, wherein the monosodium glutamate wastewater containsa great quantity of organic substances, inorganic salts, various amino acids, bacterial proteins and nucleotide degradation products, so that the monosodium glutamate wastewater can promote the growthand the development of animals and improve the immunity of the animals; the monosodium glutamate wastewater is high in interference resistance, easy to digest and absorb by animals and plants and high in biological potency, and can supplement trace elements while supplementing amino acids; copper is an essential trace element for livestock and poultry, and has a positive effect on the growth of the livestock and poultry; through various copper-containing enzymes, such as peroxidase, ceruloplasmin and dismutase, the oxidation-reduction process in organisms is influenced; and copper deficiencycan directly lead to nutritional deficiency and influence normal metabolism, so that the survival rate of the animals and plants and the utilization and conversion rate of the protein feeds can be increased after the copper is added to the protein feeds.

A compound of the active anticancer protein and bioreducer for preparing the medicine to treat cancer is prepared from the target protein chosen from transferrin, ceruloplasmin, somatostatin, vitamine bindin, etc and the bioreducer chosen from bifunctional heterocyclic nitro compound, quinone, heterocyclic NOx, topoisomerase B inhibitor and DNA-targeting medicine.

The invention belongs to the field of biological medicine application, and particularly relates to application of ceruloplasmin in preparing medicines for preventing or / and relieving or / and treating Alzheimer's disease, or / and the application of the ceruloplasmin in the aspects of preparing educational supplements or medicines. According to the application of the ceruloplasmin, the ceruloplasmin can play roles of enhancing memory ability and improving a cognitive function. The inventor finds that the ceruloplasmin can significantly promote growth of nerve cells, and can significantly improve the learning and memory ability of a dementia animal model. A cell experiment result indicates that the ceruloplasmin can effectively restrain damage of A[beta] on nerve cells, and has a protective effect for the nerve cells. A nesting experiment result indicates that the ceruloplasmin can improve the nesting ability of the dementia animal model, and reminds improvement of self-care ability of dementia animals. A maze experiment result indicates that the ceruloplasmin can improve the learning and memory ability of the dementia animal model.

The invention discloses a method for preparing intravenous injection human immune globulin from plasma component FI + III precipitates. The method comprises the following steps: dissolving the precipitate of the components I + III, adding caprylic acid to precipitate lipid protein, adsorbing miscellaneous proteins such as blood coagulation factors and ceruloplasmin in the supernatant after caprylic acid precipitation by using A50 gel, and performing one-step ion exchange chromatography, ultrafiltration preparation, nano-membrane virus removal and split charging. According to the method, IgG isextracted from precipitates of waste components FI + III, the comprehensive utilization rate of plasma is greatly increased, after a part of impure proteins such as lipoprotein and fibrinogen are precipitated with octanoic acid, a step of A50 gel is added to adsorb various blood coagulation factors in advance, various blood coagulation factors in the product are prevented from being activated inthe subsequent chromatography step, the controllability and smoothness of the process are improved, and the product has high yield and purity.

Metallo-proteins including but not limited to lactoferrin (LF), transferrin (TF) and ovotransferrin (OTF) (all members of transferrin family), ceruloplasmin (CP) and metallo-thionein (MT) were found to stabilize and enhance the bio-functional activity of tocotrienol (T3), T3 mixtures or derivates. The synergism between MP and T3 also promote the intestinal transfer and the ultimate bio-availability of T3 and T3-derivatives for physiological functions. Such functional synergism includes hypocholesterolemic, anti-thrombotic, antioxidant, anti-athermogenic, anti-inflammatory and immuno-regulatory activities of T3 agents. Addition of a non-protein-type metal chelator provided further improvement in the action of the bio-functional activity of T3. These T3 compositions are useful as pharmaceuticals, in cosmetics, in foods and as nutritional supplements.

The present invention discloses a simple and rapid immune resonance scattering spectroscopy for the determination of ceruloplasmin, which mainly makes use of the specific immune binding reaction between goat anti-human ceruloplasmin and ceruloplasmin under the condition of PEG in the 37 DEG C water bath for production of immune complex particles. The resonance scattering is existed in the complex particles. The concentration of the ceruloplasmin is linear with the strength of the resonance scattering when the concentration of ceruloplasmin is in a certain range, thereby establishing a novel method for the rapid determination of the ceruloplasmin. The present invention has the advantages that: compared with the prior art, the determination method has simple instrument, easy operation, high sensitivity and selectivity, and easy achievement of the reaction conditions. The dosage of the goat anti-human CER reagent is less and the cost is low.

The invention discloses a novel application of ceruloplasmin in treatment of multiple sclerosis, and relates to the field of treatment of multiple sclerosis. The invention discloses an application ofthe ceruloplasmin in preparation of a medicine for treating multiple sclerosis. Researches show that the ceruloplasmin has obvious treatment and improvement effects on experimental allergic encephalomyelitis (EAE), which indicates that the ceruloplasmin has a prospect of treating multiple sclerosis. The invention provides new drug selection and thought for the treatment of multiple sclerosis.

The invention discloses a ceruloplasmin detection kit which comprises a reagent R1 containing a PBS buffer solution, NaCl, NaN3, trehalose, triton and PEG-8000, with a solvent being purified water, areagent R2 containing a PBS buffer solution, NaCl, NaN3, BSA, Arabic gum and a latex-coated ceruloplasmin antibody, with a solvent being purified water, wherein the raw materials of the latex-coated ceruloplasmin antibody include latex microspheres, a ceruloplasmin antibody and a polyethylene p-chloromethyl styrene copolymer. The invention also discloses a use method of the ceruloplasmin detectionkit. The ceruloplasmin detection kit and the use method of the ceruloplasmin detection kit have the following advantages: (1) the kit provided by the invention has higher detection sensitivity and accuracy, and is simple and fast to use and operate, and detection can be completed within only 6 minutes; (2) an antigen-antibody complex formed according to the invention has high stability, certainabsorbance at a specific wavelength and strong specificity; and (3) the kit is used for a full-automatic biochemical analyzer, and can be developed and promoted on a large scale.

The invention relates to the technical field of aquatic animal breeding, and particularly discloses preparation of zinc gluconate and an application of the zinc gluconate to freshwater fish growth performance, tissue mineralization, oxidation resistance and immune response. The zinc gluconate effectively relieves oxidative stress and lipid peroxidation, can effectively stimulate the congenital and adaptive immune response of Nile tilapia, and also has stronger zinc nutrition and health promotion effects. The zinc gluconate serves as a new zinc supplement source and can promote generation of lysozyme, immune globulin M, complement 3 and complement 4. The activity of copper-zinc superoxide dismutase, fatty acid synthase, alkaline phosphatase and ceruloplasmin can be promoted, and the content of malondialdehyde in the liver can be reduced by the zinc gluconate.

Metallo-proteins including but not limited to lactoferrin (LF), transferrin (TF) and ovotransferrin (OTF) (all members of transferrin family), ceruloplasmin (CP) and metallo-thionein (MT) were found to stabilize and enhance the bio-functional activity of tocotrienol (T3), T3 mixtures or derivates. The synergism between MP and T3 also promote the intestinal transfer and the ultimate bio-availability of T3 and T3-derivatives for physiological functions. Such functional synergism includes hypocholesterolemic, anti-thrombotic, antioxidant, anti-athermogenic, anti-inflammatory and immuno-regulatory activities of T3 agents. These T3 compositions are useful as pharmaceuticals, in cosmetics, in foods and as nutritional supplements.

The invention discloses a ceruloplasmin detection kit which comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a PBS buffer solution, magnesiumchloride, trehalose, triton-100, hydroxylamine hydrochloride, sodium azide and PEG-8000; and the reagent R2 comprises the following components: a PBS buffer solution, a latex-coated copper cyanin antibody solution and bovine serum albumin. The preparation method comprises the following steps: (A) the preparation of the reagent R1: adding purified water into a blending tank A, sequentially adding the components of the reagent R1 into a blending tank, mixing and stirring, adjusting the pH value of the solution, and filtering by using a microporous filter membrane to obtain the reagent R1; and (B) the preparation of the reagent R2: adding purified water into a blending tank B, sequentially adding the components of the reagent R2 into a blending tank B, mixing and stirring, adjusting the pH value of the solution, and filtering by using a microfiltration membrane to obtain the reagent R2. By adopting the scheme disclosed by the invention, the detection efficiency is improved, and the clinical disease detection cost is reduced.