Protein marker of focal segmental glomerulosclerosis

A glomerular sclerosis and segmental technology, applied in the field of protein markers, can solve the problems of inability to distinguish the severity of the disease, early diagnosis of patient survival rate is not helpful, and not comprehensive enough

Active Publication Date: 2014-08-27
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current research mainly looks for disease markers by comparing the differences in urine protein groups between the control group and the disease group, which usually reflect the difference in urine protein at a certain time point, which has great limitations, mainly reflected in: (1) In terms of experimental design, for A study of a disease state that does not distinguish between disease severity
There is no complete and comprehensive urine proteomics data to explain the changes in the urine proteome from the beginning of the disease to renal failure, and the course of the disease cannot be diagnosed, so the practicability is not strong
(2) Most of the renal lesions found in clinical ...

Method used

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  • Protein marker of focal segmental glomerulosclerosis
  • Protein marker of focal segmental glomerulosclerosis
  • Protein marker of focal segmental glomerulosclerosis

Examples

Experimental program
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Embodiment 1

[0034] Materials and Reagents

[0035] 1) Instrument: rat metabolic cage: purchased from Beijing Jiayuan Xingye Technology Co., Ltd. TripleTOF5600 mass spectrometer: purchased from ABI Company; Agilent1200 high performance liquid chromatograph: purchased from Agilent Company; MillliQ RG ultrapure water system: purchased from Millipore Company; ): purchased from Michrom Bioresources.

[0036] 2) Main reagents: deionized water is from MillliQ RG ultrapure water system; chromatographic grade acetonitrile, formic acid and methanol are produced by Fisher; iodoacetamide (IAA), ammonium bicarbonate and dithiothreitol (DTT) are from Merck The company purchased; sequencing-grade trypsin, ConA agarose were purchased from Sigma; all antibodies were from Abcam.

[0037] 3) Animals: Male SD rats (weight 250-250 g) were purchased from Beijing Institute of Medical Experimental Animals and raised in a standard feeding environment.

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Abstract

The invention relates to a protein marker of focal segmental glomerulosclerosis, and more specifically relates to an application of a connecting part selectively connected to one or more proteins in preparing a diagnostic reagent in a development process of human focal segmental glomerulosclerosis. The one or more proteins are selected from [alpha]1 antiprotease, alpha albumin, serum albumin, beta-2-microglobulin, ceruloplasmin, fetuin-B, kininogen, serum transferrin, carboxylesterase, immune globulin gamma-2A chain C region, plasminogen, lysosomal acid phosphatase, protein YIPF3, zinc [alpha]2 glycoprotein, alpha-2-HS-glycoprotein, protein AMBP or T cell immune globulin. Specifically, the invention relates to the application of a urine protein potential marker of human focal segmental glomerulosclerosis obtained by a rat model and mass spectrometry.

Description

technical field [0001] The present invention relates to protein markers related to human focal segmental glomerulosclerosis. Specifically, the present invention relates to the use of human focal segmental glomerulosclerosis and disease severity-related protein markers obtained by using adriamycin rat nephropathy model and mass spectrometry analysis. Background technique [0002] Significance of urinary proteomics in kidney disease research: [0003] Unlike blood, which has the ability to maintain homeostasis, urine is more prone to changes, which reflect changes in body functions, that is, urine is an important source of biomarkers, see Youhe G.Can urine be the gold mine for biomarker discovery?. Sci China Life Sci2013; 43. [0004] Urine can often show the function of the urinary system to a certain extent, and it is convenient and simple to use urine changes to diagnose kidney diseases. The functional changes of the urinary system are not easy to detect, and the current...

Claims

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Application Information

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IPC IPC(8): G01N33/531
CPCG01N33/6893G01N2800/347G01N2800/52G01N2800/56
Inventor 高友鹤赵敏迪
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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