Alpha-fetoprotein detection kit

A detection kit and alpha-fetoprotein technology, applied in the direction of anti-animal/human immunoglobulin, measuring devices, instruments, etc., can solve the problems of high cost, shortage of medical equipment and personnel, and ineffective diagnosis and treatment of diseases And other problems, to achieve high sensitivity and accuracy, easy operation, low cost effect

Active Publication Date: 2020-05-05
河北渤腾医药技术有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, in large and medium-sized hospitals, the detection of tumor factors is mostly based on the chemiluminescence immunoassay method. This method is fast and accurate, but the disadvantage is that it is expensive. It must be equipped with special large-scale detection instruments and most of the detection reagents are foreign products.
However, the distribution of medical resources in our country is seriously unbalanced. There is a serious shortage of medical equipment and personnel in the vast rural areas and underdeveloped areas in the central and western regions, and there is even a lack of large-scale specialized testing instruments.
Due to the lack of medical treatment, many diseases cannot be effectively diagnosed and treated, especially cancer and other diseases are often diagnosed at an advanced stage, seriously affecting the quality of life of the people in these areas

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alpha-fetoprotein detection kit
  • Alpha-fetoprotein detection kit
  • Alpha-fetoprotein detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Monoclonal Antibody Preparation and Characteristic Analysis

[0021] 1 Materials and methods

[0022] 1.1 Animals: 7-8 weeks old female BALB / c mice were purchased from the Experimental Animal Center of Hebei Province.

[0023] 1.2 Main reagents and materials: Mouse SP2 / 0 myeloma cells are preserved by our laboratory. HAT, HT, PEG, Freund's complete adjuvant, and Freund's incomplete adjuvant are all products of Sigma. DMEM medium and cell culture plates are products of GIBCO Company. HRP-labeled goat anti-mouse IgG is a product of Huamei Bioengineering Company. All the chemical reagents used were of domestic analytical grade.

[0024] 1.3 AFP antigen: product of Sigma Company, purity>99%.

[0025] 1.4 Animal immunization: use three BALB / c mice, mix and emulsify with Freund's complete adjuvant and equal volume of AFP solution (AFP dosage is 25 μg / mouse) for the first basic immunization, and then inject at multiple points on the back of the neck, and do the ...

Embodiment 2

[0055] Embodiment 2 Development of double-antibody sandwich method ELISA detection kit

[0056] 2.1 Biotin-labeled antibody

[0057] Principle: SuLfo-NHS-LC-Biotin contains N-hydroxysuccinimide (NHS) ester, which can react with the free amino group of lysine in antibody molecules in pH 7-9 phosphate buffer, and link biotin molecules to On the antibody molecule, a stable biotin-labeled antibody complex is formed.

[0058] Operation steps: Dissolve 1mg SuLfo-NHS-LC-Biotin in 360uL ultrapure water. Take 1mg of the antibody produced by the AFP mouse monoclonal antibody hybridoma cell line 3D2 and dissolve it in 500uL pH 7.4 phosphate buffer. The two solutions were mixed and reacted at room temperature for 1 hour. After the reaction was completed, the reaction solution was put into a dialysis bag, put into 0.1M / L pH7.4 phosphate buffer, and dialyzed overnight at 4°C.

[0059] 2.2 ELISA detection process

[0060] For coating, the AFP antigen was coated at a concentration of 5 u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to view more

Abstract

The invention provides an alpha fetoprotein monoclonal antibody, which is generated by hybridoma with a preservation number of CGMCC No. 13808. The alpha fetoprotein detection kit comprises a coating solution, the antibody generated by hybridoma with the preservation number of CGMCC No. 13808, alpha fetoprotein antigen, an enclosing solution, washing liquid, a biotin-labeled antibody generated by hybridoma with the preservation number of CGMCC No. 13808, horseradish peroxidase-labelled avidin, a substrate colored solution and a stopping solution. According to the invention, AFP mice monoclonal antibody hybridoma cell strain with the preservation number of CGMCC No. 13808 is obtained, the generated antibody can be used for detecting AFP antigen, has high sensitivity and accuracy, and has the advantages of low cost and simple operation while being used for early diagnosis of liver cancer.

Description

technical field [0001] The invention belongs to the technical field of kits, in particular to a kit for detecting alpha-fetoprotein. Background technique [0002] At present, malignant tumors have ranked first in the death rate of major diseases among residents in our country, and have become the number one killer affecting people's health. According to statistics from the Ministry of Health of my country, there are about 3.37 million new cancer cases and 2.11 million deaths in my country every year. The incidence rate of malignant tumors in the tumor registration areas of Hebei Province is 224.59 / 100,000. Liver cancer (especially primary hepatocellular carcinoma, Hepatocellular carcinoma, HCC) has become the second most common cancer after gastric cancer. As a disease with high mortality, the etiology, development, recurrence, metastasis, diagnosis and treatment of liver cancer have attracted more and more attention. [0003] Statistics from the World Health Organization ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18G01N33/574G01N33/577
CPCC07K16/18G01N33/57476G01N33/57484G01N33/577
Inventor 许峰程华温占波纪振动郝鹏范朝霞
Owner 河北渤腾医药技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap