Alpha-fetoprotein detection kit
A detection kit and alpha-fetoprotein technology, applied in the direction of anti-animal/human immunoglobulin, measuring devices, instruments, etc., can solve the problems of high cost, shortage of medical equipment and personnel, and ineffective diagnosis and treatment of diseases And other problems, to achieve high sensitivity and accuracy, easy operation, low cost effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] Example 1 Monoclonal Antibody Preparation and Characteristic Analysis
[0021] 1 Materials and methods
[0022] 1.1 Animals: 7-8 weeks old female BALB / c mice were purchased from the Experimental Animal Center of Hebei Province.
[0023] 1.2 Main reagents and materials: Mouse SP2 / 0 myeloma cells are preserved by our laboratory. HAT, HT, PEG, Freund's complete adjuvant, and Freund's incomplete adjuvant are all products of Sigma. DMEM medium and cell culture plates are products of GIBCO Company. HRP-labeled goat anti-mouse IgG is a product of Huamei Bioengineering Company. All the chemical reagents used were of domestic analytical grade.
[0024] 1.3 AFP antigen: product of Sigma Company, purity>99%.
[0025] 1.4 Animal immunization: use three BALB / c mice, mix and emulsify with Freund's complete adjuvant and equal volume of AFP solution (AFP dosage is 25 μg / mouse) for the first basic immunization, and then inject at multiple points on the back of the neck, and do the ...
Embodiment 2
[0055] Embodiment 2 Development of double-antibody sandwich method ELISA detection kit
[0056] 2.1 Biotin-labeled antibody
[0057] Principle: SuLfo-NHS-LC-Biotin contains N-hydroxysuccinimide (NHS) ester, which can react with the free amino group of lysine in antibody molecules in pH 7-9 phosphate buffer, and link biotin molecules to On the antibody molecule, a stable biotin-labeled antibody complex is formed.
[0058] Operation steps: Dissolve 1mg SuLfo-NHS-LC-Biotin in 360uL ultrapure water. Take 1mg of the antibody produced by the AFP mouse monoclonal antibody hybridoma cell line 3D2 and dissolve it in 500uL pH 7.4 phosphate buffer. The two solutions were mixed and reacted at room temperature for 1 hour. After the reaction was completed, the reaction solution was put into a dialysis bag, put into 0.1M / L pH7.4 phosphate buffer, and dialyzed overnight at 4°C.
[0059] 2.2 ELISA detection process
[0060] For coating, the AFP antigen was coated at a concentration of 5 u...
PUM
Property | Measurement | Unit |
---|---|---|
Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com