Affinity adsorption measuring method of alpha-feto-protein heteroplasmon

A technology for the measurement of alpha-fetoprotein, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long measurement time, large result variation, high cost, etc., and achieve the effect of low measurement cost, reliable results and simple method

Inactive Publication Date: 2005-03-23
HANGZHOU FIRST PEOPLES HOSPITAL
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Problems solved by technology

[0004] The purpose of the present invention is to provide an affinity adsorption assay method for alpha-fetoprotein heterogeneity that solves the shortcomings of long measurement time, cumbersome operation, high cost, and large variation in results in the determination of AFP heterogeneity

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  • Affinity adsorption measuring method of alpha-feto-protein heteroplasmon

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Embodiment Construction

[0010] The present invention will be further described below in conjunction with the embodiments and the accompanying drawings.

[0011] Materials and Methods:

[0012] 1. Samples of 58 cases of various chronic liver diseases (diagnosed clinically and combined with B-ultrasound, CT, and immunoassay, (52 males, 6 females, aged 26-68, all from Hangzhou Infectious Disease Hospital), 42 cases of primary Hepatocellular carcinoma (37 males, 5 females, aged 26-72 years, from our hospital, Zhejiang Cancer Hospital and Hangzhou Hospital of Infectious Diseases). 32 normal pregnant women (all from our hospital).

[0013] 2. Reagents

[0014] 1. Buffer 1: 0.05mol / L Tris-HCl (containing 0.5mol / L NaCl), pH7.6

[0015] 2. Buffer 2: 0.01mol / L Tris-HCl (containing 0.5mol / L NaCl), pH7.6, then add 1mmol / LMgCl 2 , 1mmol / L CaCl 2 , 1mmol / L MnCl 2

[0016] 3. Lentil Lectin Sepharose 4B (Lentil Lectin Sepharose 4B), Code No.17-0444-01, lot.290573, est exp 2004.08, Pharmacia Biotech.

[0017] ...

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Abstract

An affinity adsorption method for detecting alpha-fetoglobuline heteroplasmon includes such steps as mixing affinity adsorbent with buffering liquid, centrifugal separation, removing supernatant, mixing it with diluted specimen, waving centrifugal treating, measuring the AFP in supernatant, diluting specimen, measuring total AFP, and calculating the AFP binding rate of each specimen to obtain each AFP gloco-chain heteroplasmon. Its advantages are high speed and reliability and low cost.

Description

technical field [0001] The invention relates to a determination method, in particular to an affinity adsorption determination method of alpha-fetoprotein heterogeneity. Background technique [0002] Since Purves discovered AFP heterogeneity in the serum of patients with liver cancer by starch gel electrophoresis in 1970[3], many people have done a lot of in-depth research on this, and the classification and clinical evaluation of AFP heterogeneity have made great progress[4,5 ]. The determination methods in China are mainly limited to two kinds[6,7]: affinity cross-immunoelectrophoresis autoradiography and affinity electrophoresis immunoblotting. These two methods have many steps, take a long time, and require special equipment to judge the results. This greatly limits the clinical application of AFP heterogeneity determination. Qi Weimin et al. (2001) proposed to cut off a certain position on the gel after affinity electrophoresis [8] and add enzyme-linked immunosorbent a...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N30/02G01N33/574G01N33/576
Inventor 汪子伟
Owner HANGZHOU FIRST PEOPLES HOSPITAL
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