A kit for the quantitative detection of alpha-fetoprotein variant (afp-l3) by immunofluorescence test strip dominated by functional magnetic spheres

A technology of AFP-L3 and immunofluorescence, which is applied in the field of kits for quantitative detection of AFP-L3 by immunofluorescence test strips, can solve the problems of conjugate retention, cumbersome operation, and insufficient elution, and achieve small interference factors and easy operation easy effect

Active Publication Date: 2020-11-24
陈复华
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  • Description
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Problems solved by technology

[0009] The shortcomings of the spin column adsorption method for detecting AFP-L3 are cumbersome operation (repeated centrifugation), long time-consuming (1.5-2 hours), and there may be a small amount of conjugates left behind due to insufficient elution or improper centrifugation speed control. and other problems, resulting in a decrease in the recovery rate and thus affecting the detection results

Method used

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  • A kit for the quantitative detection of alpha-fetoprotein variant (afp-l3) by immunofluorescence test strip dominated by functional magnetic spheres
  • A kit for the quantitative detection of alpha-fetoprotein variant (afp-l3) by immunofluorescence test strip dominated by functional magnetic spheres
  • A kit for the quantitative detection of alpha-fetoprotein variant (afp-l3) by immunofluorescence test strip dominated by functional magnetic spheres

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preparation example Construction

[0029] The present invention also provides a method for preparing the above-mentioned functional magnetic balls, comprising the following steps: 1) mixing the magnetic beads, LCA and a condensing agent and reacting for 0.5-48 hours to obtain a reaction solution; 2) magnetically separating the reaction solution and collecting the magnetic group points to obtain functional magnetic balls.

[0030] In the present invention, the magnetic beads, LCA and the condensing agent are mixed and reacted for 0.5 to 48 hours to obtain a reaction solution. The mass ratio of the magnetic beads to the LCA is 1000:(0.5~1), more preferably 1000:(0.6~1). 0.9), most preferably 1000:(00.7~0.8); the mass ratio of the magnetic beads to the condensing agent is (5:1)~(1:20). In the present invention, the magnetic beads, LCA and the condensing agent are mixed and reacted according to the above ratio, and the reaction time is preferably 1-40 h, more preferably 2-30 h. The condensing agent described in th...

Embodiment 1

[0045] Take 1 mL of amino or carboxylated magnetic beads (particle size 300 nm) solution with a solid content of 20% and mix with 1 mL of the solution containing 2 mg of lentil lectin, add 5 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbon Diimine hydrochloride, mixed evenly, stirred and reacted at room temperature for 48 hours, magnetically separated and washed to obtain functional magnetic spheres.

[0046] Buffer solution configuration: Weigh 3.0g tris (Tris), 1.45g sodium chloride, 0.4g magnesium chloride, 0.22g calcium chloride and dissolve them in 950ml distilled water, adjust the pH to 7.5, and finally add distilled water to determine Make up to 1000ml to obtain a buffer solution.

[0047] The functional magnetic ball, buffer solution, and AFP immunofluorescence quantitative test strip are assembled into an AFP-L3 detection kit.

Embodiment 2

[0049] Take 1 mL of amino or carboxylated magnetic beads (particle size 10000nm) solution with a solid content of 20% and mix with 1 mL of a solution containing 2 mg of lentil lectin, add 5 mg of dicyclohexylcarbodiimide, mix well, and stir at room temperature for 24 hours. , the functional magnetic spheres were obtained after magnetic separation and washing.

[0050] Buffer solution configuration: Weigh 3.0g tris (Tris), 1.45g sodium chloride, 0.4g magnesium chloride, 0.22g calcium chloride and dissolve them in 950ml distilled water, adjust the pH to 7.4, and finally add distilled water to determine Make up to 1000ml to obtain a buffer solution.

[0051] The functional magnetic ball, buffer solution, and AFP immunofluorescence quantitative test strip are assembled into an AFP-L3 detection kit.

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Abstract

The invention provides a functional magnetic sphere dominated kit for quantitatively detecting an alpha fetoprotein variant (AFP-L3) through an immunofluorescence test strip. The kit for detecting theAFP-L3 comprises a functional magnetic sphere solution, a buffering solution and an alpha fetoprotein (AFP) immunofluorescence test strip; a functional magnetic sphere is a magnetic sphere of lens culinaris agglutinin (LCA). The characteristic of specific combination with the AFP-L3 of the functional magnetic sphere is used, the difference of the AFP concentrations of one sample adsorbed by the functional magnetic sphere and the other sample not absorbed by the functional magnetic sphere is rapidly quantitatively detected through the AFP immunofluorescence test strip, and the result of the AFP-L3 is acquired. The kit created by aiming at the characteristic of the clinical detection of the AFP-L3 by one hundred percent is convenient to operate, and is fast, stable and accurate.

Description

technical field [0001] The invention belongs to the technical field of immunodetection, in particular to a kit for quantitatively detecting AFP-L3 with immunofluorescence test strips dominated by functional magnetic spheres. Background technique [0002] Worldwide, hepatitis B virus, hepatitis C virus hepatitis and liver cirrhosis are important risk factors for liver cancer (HCC), and more than 90% of HCC patients are detected with hepatitis B virus. my country is a country with a high incidence of HCC. Clinically, HCC is often found at an advanced stage, which is difficult to treat, has many surgical complications, usually has poor curative effects, and has a short survival period. Generally, the survival time after the onset of the disease is only 6 months to one year. Therefore, in the prevention and treatment of liver cancer, early detection, early diagnosis and early treatment are particularly important. [0003] Alpha-fetoprotein (AFP) has been used for many years as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574G01N33/543
CPCG01N33/54326G01N33/57438G01N33/6893
Inventor 陈复华
Owner 陈复华
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