Immune syntony scattering spectrometry for rapid measuring copper blue protein

A ceruloplasmin and resonance scattering technology, which is applied in the field of immune resonance scattering spectroscopy for rapid determination of ceruloplasmin, can solve the problems of lack of comparability of results, large amount of antiserum, low sensitivity, etc. Easy conditions and good selectivity

Inactive Publication Date: 2008-04-09
GUANGXI NORMAL UNIV
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Problems solved by technology

At present, there are many detection methods for CER, such as the application of p-phenylenediamine (PPD) method according to its oxidase characteristics; according to its immunological characteristics, the measurement methods include enzyme-linked immunosorbent assay, radioimmunoassay, immunoprecipitation colorimetric analysis, Rocket immunoelectrophoresis analysis, immunoelectric diffusion analysis, in addition, there are pulsed laser acoustic analysis, application rate scattering turbidimetry, etc. Some of these methods are not high in sensitivity, some have cumbersome steps, and some are due to matrix, reaction conditions, etc. Inconsistent, but the results lack comparability, and some antisera use a large amount, and the purity requirements are high, so it is not easy to operate

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  • Immune syntony scattering spectrometry for rapid measuring copper blue protein

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Embodiment

[0021] A kind of immunoresonance scattering spectrometry that can measure ceruloplasmin rapidly comprises the following steps:

[0022] (1) Prepare the tested system with known CER concentration, pipette 0.30mL phosphate buffer solution with a pH value of 6.4 into five 5mL graduated test tubes with a micro sampler, add 0.20mL diluent to each test tube 25 times of goat anti-human CER solution (provided by Shanghai Beika Reagent Co., Ltd.), and then use a micro sampler to pipette 0, 5, 60, 120, and 180 μL of CER solution with a concentration of 10.8 μg / mL and inject them into 5 test tubes respectively. Then add 0.50 mL of PEG-6000 solution with a mass concentration of 15%, dilute to 2.0 mL with twice distilled water, mix well, and incubate in a 37°C water bath for 15 min;

[0023] (2) According to the method of step (1), the reagent blank system is prepared without adding CER;

[0024] (3) Take an appropriate amount of each in different quartz cells, and scan the excitation wav...

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Abstract

The present invention discloses a simple and rapid immune resonance scattering spectroscopy for the determination of ceruloplasmin, which mainly makes use of the specific immune binding reaction between goat anti-human ceruloplasmin and ceruloplasmin under the condition of PEG in the 37 DEG C water bath for production of immune complex particles. The resonance scattering is existed in the complex particles. The concentration of the ceruloplasmin is linear with the strength of the resonance scattering when the concentration of ceruloplasmin is in a certain range, thereby establishing a novel method for the rapid determination of the ceruloplasmin. The present invention has the advantages that: compared with the prior art, the determination method has simple instrument, easy operation, high sensitivity and selectivity, and easy achievement of the reaction conditions. The dosage of the goat anti-human CER reagent is less and the cost is low.

Description

Technical field: [0001] The invention relates to a protein assay method, in particular to an immunoresonance scattering spectrometry method capable of rapidly assaying ceruloplasmin. Background technique: [0002] Ceruloplasmin belongs to alpha 2 -Globulin (CER), a copper-containing alpha-glycoprotein, is the only blue protein in humans and vertebrates. At present, there are many detection methods for CER, such as the application of p-phenylenediamine (PPD) method according to its oxidase characteristics; according to its immunological characteristics, the measurement methods include enzyme-linked immunosorbent assay, radioimmunoassay, immunoprecipitation colorimetric analysis, Rocket immunoelectrophoresis analysis, immunoelectric diffusion analysis, in addition, there are pulsed laser acoustic analysis, application rate scattering turbidimetry, etc. Some of these methods are not high in sensitivity, some have cumbersome steps, and some are due to matrix, reaction condition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/541G01N21/63
Inventor 韦丽丽蒋治良
Owner GUANGXI NORMAL UNIV
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