Preparation of zinc gluconate and application of zinc gluconate to freshwater fish growth performance, tissue mineralization, oxidation resistance and immune response

A zinc gluconate, growth performance technology for applications, climate change adaptation, animal feed, etc., which can solve problems such as limited data, unclear tissue mineralization and immune health biological activity

Pending Publication Date: 2021-04-23
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are limited data on Glu-Zn as a zinc source supplement in aquatic animals, especially the bioactivity of Glu-Zn on growth performance, tissue mineralization and immune health of Nile tilapia (Oreochromis niloticus) is unclear

Method used

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  • Preparation of zinc gluconate and application of zinc gluconate to freshwater fish growth performance, tissue mineralization, oxidation resistance and immune response
  • Preparation of zinc gluconate and application of zinc gluconate to freshwater fish growth performance, tissue mineralization, oxidation resistance and immune response
  • Preparation of zinc gluconate and application of zinc gluconate to freshwater fish growth performance, tissue mineralization, oxidation resistance and immune response

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method for zinc gluconate (Glu-Zn), comprising the following steps:

[0038] S1. Weigh 5g sodium gluconate and 3.7g ZnSO 4 ·7H 2 O was dissolved in 10 mL deionized water to form a mixed solution, and the mixed solution was magnetically stirred at 50°C for 30 minutes;

[0039] S2. Use 0.1mol / L NaOH solution to adjust the pH of the mixed solution to 5.4, add 3mL of ethanol, and let it stand for 30 minutes to obtain a solid product precipitation;

[0040] S3. Wash the solid product precipitate with absolute ethanol, wash three times, and vacuum-dry at 50° C. for 24 hours to obtain a solid Glu-Zn complex, namely zinc gluconate (Glu-Zn).

[0041] In other embodiments, a 0.1 mol / L NaOH solution can be used to adjust the pH of the mixed solution to 5, 5.5 or 6.

[0042] For structural characterization, Fourier transform infrared (FTIR) spectrometer (Spectrum 100, PerkinElmer Inc., USA) at 450-4000 cm -1 Glu-Na and Glu-Zn complexes were analyzed in the range. ...

Embodiment 2-6

[0050] The formula of a feed is shown in Table 2.

[0051] Table 2

[0052]

[0053]

[0054] Wherein the vitamin premix in Example 2-6 includes 12mg vitamin B1, 0.05mg vitamin B12, 8mg vitamin B6, 30mg niacin, 5mg vitamin D3, 100mg vitamin C, 50mg vitamin E, 40mg pantothenic acid, 0.8mg vitamin H, 5mg vitamin B, 25mg vitamin A, 8mg vitamin K3, 12mg riboflavin and 100mg inositol.

[0055] Zinc-deficient multi-mineral premix includes 400mg FeSO4·H 2 O, 200mg KCl, 150mg MnSO 4 ·H 2 O, 60mg KI, 65mg Na2SeO 3 ·5H 2 O and 30 mg CuSO 4 ·5H 2 O.

[0056] In addition, the feed approximate composition of embodiment 4-6 is shown in table 3:

[0057] table 3

[0058] approximate composition Example 4 Example 5 Example 6 Crude protein (%) 38.75 37.50 38.89 Crude fat (%) 7.80 7.67 7.63 Ash (%) 10.03 9.84 10.07 Moisture (%) 6.94 9.32 7.06 Zinc content (mg / kg) 47.08 49.79 45.54

Embodiment 7

[0059] Embodiment 7, Glu-Zn complex is to the biological activity experiment of Nile tilapia

[0060] Experimental materials: Examples 5 and 6 are used as control groups, and Example 4 is used as an experimental group.

[0061] 1. Feeding and management of fish: Tilapia juveniles were domesticated for 2 weeks in a circular fiber tank (400 liters), and fed with a basic diet to adapt to the experimental diet and conditions. At the beginning of the experiment, the adapted fish were fasted for 24 hours, and then, 180 healthy fish larvae (77.03±0.14 g) were randomly divided into three groups, each group had three replicates, and each replicate had 20 fish. The three groups were fed the feed of Example 5, the feed of Example 6 and the feed of Example 4 respectively. The 18 experimental fish tanks were carried out under the natural light and dark cycle, and the experimental cycle was 60 days. The weight of the fish food fed daily was fed at 4% of the fish body weight, and was adjus...

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PUM

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Abstract

The invention relates to the technical field of aquatic animal breeding, and particularly discloses preparation of zinc gluconate and an application of the zinc gluconate to freshwater fish growth performance, tissue mineralization, oxidation resistance and immune response. The zinc gluconate effectively relieves oxidative stress and lipid peroxidation, can effectively stimulate the congenital and adaptive immune response of Nile tilapia, and also has stronger zinc nutrition and health promotion effects. The zinc gluconate serves as a new zinc supplement source and can promote generation of lysozyme, immune globulin M, complement 3 and complement 4. The activity of copper-zinc superoxide dismutase, fatty acid synthase, alkaline phosphatase and ceruloplasmin can be promoted, and the content of malondialdehyde in the liver can be reduced by the zinc gluconate.

Description

technical field [0001] The invention relates to the technical field of aquatic animal breeding, in particular to the preparation of zinc gluconate and its application in freshwater fish growth performance, tissue mineralization, anti-oxidation and immune response. Background technique [0002] Intensive aquaculture is rapidly developing in many countries, especially in developing countries, as the world population increases and the demand for protein increases. Nile tilapia (Oreochromis niloticus, O. niloticus) is the most important farmed fish in the world, mainly cultured in China, Indonesia and Egypt, with a global production of about 5.9 million tons. Although the tilapia industry has grown rapidly in recent years, fish farmers still need to address many challenges, including pathogen control, fish nutrient supplementation, and water environmental impacts. To date, most research on developing effective methods of disease prevention has focused on the use of chemotherapy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/20A23K20/163A23K10/22A23K10/30A23K10/38A23K20/158A23K20/26A23K20/24A23K20/174A23K20/22A23K20/105
CPCY02P60/87Y02A40/818
Inventor 舒绪刚罗帆杨富杰付志欢杨红艳田允波邹仕庚
Owner ZHONGKAI UNIV OF AGRI & ENG
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