50results about How to "Relieve oxidative stress" patented technology

The invention discloses lactobacillus paracasei capable of relieving gastrointestinal injury caused by capsaicin and an application of the lactobacillus paracasei, and belongs to the technical field of microorganisms. The lactobacillus paracasei CCFM1176 can relieve oxidative stress of the mouse liver caused by capsaicin and reduce the level of serum neuropeptide of the mouse liver. The damages such as gastric mucosa cell vacuolation, intestinal villus shedding, colon goblet cell reduction and inflammatory cell infiltration caused by capsaicin can be improved; and the transcriptional level of mouse gastrointestinal tissue inflammation marker protein can be reduced. The lactobacillus paracasei CCFM1176 is used for preparing a pharmaceutical composition and fermented food for relieving pungency and protecting intestines and stomach, and has a very wide application prospect.

The invention discloses a preparation method and application of a bulbus fritillariae cirrhosae extract. The method comprises the following steps: pulverizing bulbus fritillariae cirrhosae, soaking the bulbus fritillariae cirrhosae in an alcoholic solution overnight, carrying out continuous reflux extraction at 60-90 DEG C, collecting an extracted concentrated solution, removing a solvent to obtain a concentrated extract, and carrying out reduced-pressure vacuum drying to obtain the bulbus fritillariae cirrhosae extract. Alkaloids in the bulbus fritillariae cirrhosae extract comprise sipeimine, peiminine, peimine, edpetiline, peimine, peimisine, sinpeinine A, yibeinoside A and ebeiedinone. The bulbus fritillariae cirrhosae extract disclosed by the invention can be used as a medicine for treating chronic respiratory system diseases, including chronic obstructive pneumonia, chronic pharyngitis and pulmonary fibrosis, and can activate MAPKs and Nrf2 signal channels.

The invention discloses a feed additive for preventing lactation-period-calf diarrhea and an application method thereof. According to the feed additive, natural astaxanthin, sodium humate, beta-hydroxyl-beta-methylbutyric acid, glutamine, xylooligosaccharide and a Chinese herbal medicine additive (agrimonia pilosa ledeb, honeysuckle, Chinese pulsatilla roots, hawthorns, pomegranate bark, talc andlicorice roots) are compounded, the balance of gastrointestinal flora of lactation-period calves is conditioned, development of gastrointestinal mucosa is promoted, the damage and influence of pathogens and unfavorable factors on the calves are reduced, digestion and absorption of nutritional ingredients are increased, the body immunity is improved, the suffered oxidation stress and oxidative damage are relieved, the anti-stress ability is improved, and therefore the aims that occurrence of lactation-period-calf diarrhea is avoided, absorption of feed nutrients is promoted, and the health level and production performance of the calves are improved are achieved.

The invention discloses an antioxidant peptide and a preparation method and application thereof, and aims to provide an antioxidant peptide which is derived from chicken breast myofibrillar protein and has high-efficiency antioxidant activity, and a preparation method and application thereof. The amino acid sequence of the antioxidant peptide is ITTNPYDY or IGWSPLGSL. The preparation method of theantioxidant peptide comprises the following steps of: extracting the myofibrillar protein from fresh chicken breast serving as a raw material; performing enzymolysis on the obtained myofibrillar protein by using pepsin; and performing separation, purification and synthesis to obtain the antioxidant peptide. The antioxidant peptide is a novel antioxidant peptide, and has high antioxidant activitythrough detection; under the measuring condition, the iron ion reducing capacity and the oxygen radical absorbing capacity of the antioxidant peptide are obviously higher than those of glutathione; and meanwhile, the antioxidant peptide has an obvious effect of inhibiting the oxidative damage of NIH-3T3 cells. The antioxidant peptide belongs to a natural antioxidant, is safer to eat, and can be applied to food, health-care products and medicaments with antioxidant functions.

The invention discloses low-sugar weight-losing coffee and a preparation method thereof. The low-sugar weight-losing coffee comprises the following components in percentage by weight: 30%-60% of coffee powder, 10%-30% of skim milk powder, 10%-30% of inulin, 0.6%-3% of L-carnitine, 0.1%-5% of lotus leaves, 0.1%-5% of haws, 0.1%-5% of cassia seeds, 0.1%-5% of hemp seeds, 0.1%-5% of corn stigma and 0.01%-1.0% of sucralose. The product belongs to a sugar-free low-sugar product, is smooth in taste, rich in coffee flavor and rich in taste, and has the remarkable effects of refreshing, satiety, losing weight, clearing internal heat, improving intestinal tracts and relieving fatigue. The tea is instant, convenient and fast; the low-sugar weight-losing coffee is suitable for all weight-reducing people.

The invention belongs to the technical field of new applications of drugs, and particularly relates to an application of aspirin in preparation of drugs for treating IVDD (intervertebral disc degeneration). An intervertebral disc degeneration model is constructed by applying LPS (lipopolysaccharide)-induced oxidative stress, the therapeutic effect of aspirin on IVDD is observed, and the therapeutic effect of Aspirin on IVDD is evaluated by observing oxidative stress and various indexes of IVDD. Western blot demonstrates the brief mechanism of Aspirin in reducing oxidative stress and alleviating IVDD. IVDD is caused by oxidative stress in intervertebral disc nucleus pulposus tissue, Aspirin delays IVDD by inhibiting the oxidative stress in the nucleus pulposus tissue and further reducing decomposition of cell matrix and type II collagen, so that a new approach is provided for prevention and treatment of IVDD.

The invention discloses feed for late pregnancy of sows and a feeding method. The feed is characterized by being prepared from the following components in parts by weight: 55 to 65 parts of corn, 15 to 25 parts of soybean meal, 5 to 10 parts of wheat bran, 1 to 2 parts of soybean oil, 1 to 2 parts of glucose, 0.5 to 1 part of table salt, 1 to 2 parts of mountain flour, 1 to 2 parts of dicalcium phosphate, 0.3 to 0.5 part of lysine, 0.4 to 0.8 part of threonine, 4 to 7 parts of dietary fiber, 2 to 4 parts of plant extract, 0.1 to 0.3 part of choline chloride and 3 to 4 parts of sow compound premix. According to the feed disclosed by the invention, when the seed is used for producing by comprehensively considering physiological characteristics of pregnancy, the oxidative stress of the late pregnancy of the sows can be alleviated, the rate of weak piglets and dead fetuses of the sows is reduced, the quantity of healthy piglets is increased and the production benefits are easy to improve;the oxidative stress of the late pregnancy can be alleviated and the pregnancy metabolism is alleviated at maximum, so that the oxidative stress of the late pregnancy of the sows is alleviated and thefetal birth health and uniformity are ensured; furthermore, certain antibiotics are replaced with the plant extract in the product, so that the addition of medicines in the feed is greatly reduced.

The present invention provides a premix for preventing and treating fatty liver of dairy cattle in a perinatal period and a related application thereof. The premix simultaneously contains a certain amount of ruminal choline and selenium, creatively utilizes a combination of the two materials to have a double protection function for liver, groundbreakingly realizes a synergic effect of the two materials added in the premix at the same time, is prefect in liver protection, antioxidant and lactating performances of the dairy cattle in the perinatal period, and realizes an one-plus-one-is-greater-than-two effect.

The invention discloses a nutrient supplement for egg-laying hens, capable of shortening the period from egg laying starting time to egg laying peak time, as well as a preparation method and application of the nutrient supplement, and solves the problem that in the prior art, a nutrient supplement capable of shortening the period from egg laying starting time to egg laying peak time does not exist. The nutrient supplement for egg-laying hens contains 100-150 parts of epidermal growth factors, 50-100 parts of probiotics, 60-150 parts of chitosan oligosaccharide, 50-120 parts of a compound enzyme preparation for poultry, 50-120 parts of calcium lactate, 50-100 parts of thyme, 50-100 parts of an acidulant, 150-200 parts of vitamin E, 10-15 parts of vitamin A, 2-3 parts of vitamin D3, and 100-600 parts of refined rice bran meal. The preparation method comprises the following steps of weighing the components in parts by weight, performing uniform mixing and performing subpacking so as to obtain the nutrient supplement. According to the application of the nutrient supplement, 0.15-0.5% of the nutrient supplement for egg-laying hens is added to a basal feed. According to the nutrient supplement disclosed by the invention, the period from the egg laying starting time to the egg laying peak time can be shortened.

The invention provides mixed probiotics for soybean meal fermentation. The mixed probiotics are made by mixing of, by weight, 3-5 parts of lactobacillus reuteri, 3-5 parts of lactobacillus plantarum,1-3 parts of bacillus subtilis and 1-3 parts of aspergillus oryzae. The invention further provides a method for soybean meal fermentation by the mixed probiotics and further provides a fermented product obtained by fermentation according to the fermentation method. The mixed probiotics for soybean meal fermentation and the fermentation method have advantages that various anti-nutritional factors in soybean meal can be degraded completely, and feed digestibility of animals can be improved by adding of the mixed probiotics into feeds; due to rich contents of probiotics and beneficial metabolites, part of antibiotic functions can be achieved by adding of the mixed probiotics into feeds, oxidative stress of pig herds can be relieved helpfully, animal product quality is improved, and animal productivity and immunity can be improved.

The invention discloses a bacillus paralicheniformis and a method for preparing bio-nano-selenium-enriched probiotics. Bacillus paralicheniformis, named Bacillus paralicheniformis ZJUSE‑1, was preserved in the China General Microorganism Culture Collection and Management Center on March 21, 2017, with a preservation number: CGMCC No.13908. After fermentation, bio-nano selenium-rich probiotics were obtained. Bio-nanometer selenium is coated with secretions containing polysaccharides and protein characteristic groups, has antioxidant activity, and can scavenge DPPH free radicals and superoxide anions. Bio-nanometer selenium and probiotics play a synergistic effect, which can effectively repair the oxidative damage of the body, relieve the oxidative stress caused by weaning of the young, and improve the economic benefits of breeding production.

The invention provides application of geraniol and a derivative thereof in preparing drugs for MRSA infectious diseases. The invention further provides a pharmaceutical composition for treating drug-resistant staphylococcus infectious diseases. The invention finally provides combination drugs for treating the drug-resistant staphylococcus infectious diseases. The combination drugs comprise the geraniol which has unit preparations of the same or different specifications and is used for dosing simultaneously or separately and drugs for treating the drug-resistant staphylococcus infectious diseases. The geraniol and the derivative thereof are natural drugs, for sing use, growth of MRSA can be effectively inhibited or MRSA is killed, an oxidative stress and inflammatory reaction caused by MRSAinfection can be relieved, and the geraniol and the derivative thereof can be used for treating the staphylococcus infectious diseases; meanwhile by combining beta-lactam antibiotics, the activity ofthe MRSA can be significantly enhanced, and the good synergistic effect is achieved; the drugs are prepared to be drugs for treating MRSA infection, and the clinical application prospect is good.

The invention discloses a fattening pig premix additive capable of improving meat quality and a preparation method thereof, and belongs to the field of feed additives. The feed is prepared from the following raw materials according to every 2000 parts by weight: 30-90 parts of herba gracilariae asiaticae, 30-90 parts of common seepweed herb, 50-80 parts of ulva pertusa kjellm, 40-100 parts of fructus crataegi, 40-120 parts of radix astragali, 40-120 parts of serine, 40-120 parts of glycine, 50-110 parts of leucine, 50-110 parts of isoleucine, 100-300 parts of soybean oil, 200-400 parts of linseed oil and a carrier. Only a trace of the premix additive is added to the feed, a pork color, drop loss, tenderness, intramuscular fat content and muscle fiber type compositions can be effectively improved, and a pork flavor, a mouthfeel and a nutritive value are improved. The premix additive is safe and no side effects in source of raw materials, and pollution-free to an environment.

The present invention provides nanoformulations for topical and systemic fat reduction and uses thereof. The nano preparation consists of an oil phase and a water phase. The oil phase is selected from triglyceride and other membrane material components, an oxidative stress inhibitor vitamin E, an endoplasmic reticulum UPR pathway inhibitor and the like, and the water phase is selected from a vitamin C deionized water solution and the like. According to the nano preparation, the endoplasmic reticulum stress inhibitor is entrapped in the carrier containing the oxidative stress inhibitor, so that the fat differentiation of precursor cells and the oxidative stress and the endoplasmic reticulum stress which are necessary in the maturation process of fat cell accumulated lipid are weakened, the new fat source is greatly reduced, and the overgrowth of the existing fat cells is inhibited; the purpose of efficient and safe slimming is achieved. Different purposes of local and / or systemic fat reduction can be realized due to different administration modes, administration sites and administration dosages. In addition, the traditional Chinese medicine also has a certain effect on preventing / treating fatty liver. The method is reasonable in design, easy to prepare, safe, efficient and easy to popularize.

The invention belongs to food and particularly relates to application of a ganoderma lucidum polysaccharide compound in relieving toxicity of cyclophosphamide (CYP). The ganoderma lucidum polysaccharide compound comprises ganoderma lucidum polysaccharide and waxy corn starch, wherein the mass ratio of the ganoderma lucidum polysaccharide to the waxy corn starch is 1:4. The prepared ganoderma lucidum polysaccharide and corn starch large-particle compound has functions of effectively relieving oxidation stress and liver injury caused by the CYP. The compound shows a certain anti-digestion characteristic due to physical functions of corn starch and ganoderma lucidum polysaccharide, overcomes defects of in-vivo digestion of ganoderma lucidum polysaccharide, and effectively improves the utilization rate and biotransformation rate of the ganoderma lucidum polysaccharide.

The invention relates to the technical field of aquatic animal breeding, and particularly discloses preparation of zinc gluconate and an application of the zinc gluconate to freshwater fish growth performance, tissue mineralization, oxidation resistance and immune response. The zinc gluconate effectively relieves oxidative stress and lipid peroxidation, can effectively stimulate the congenital and adaptive immune response of Nile tilapia, and also has stronger zinc nutrition and health promotion effects. The zinc gluconate serves as a new zinc supplement source and can promote generation of lysozyme, immune globulin M, complement 3 and complement 4. The activity of copper-zinc superoxide dismutase, fatty acid synthase, alkaline phosphatase and ceruloplasmin can be promoted, and the content of malondialdehyde in the liver can be reduced by the zinc gluconate.

The invention discloses an immunoenhancer for a pregnant sow. The immunoenhancer is prepared from the following components in parts by weight: 15-25 parts of Chinese herbal medicine extract, 4-8 parts of fish meal, 2-4 parts of stone powder, 2-4 parts of calcium monohydrogenphosphate, 5-9 parts of glucose, 1.5-2.5 parts of lysine, 1.5-2.5 parts of methionine, 1.5-2.5 parts of threonine, 1.5-2.5 parts of arginine, 2-4 parts of beta-hydroxyl-beta-methylbutyric acid, 2-4 parts of acidifier, 2-4 parts of enzyme preparation, 1.5-2.5 parts of vitamin complex, 3-5 parts of probiotic complex and 35-40 parts of starch accessory, wherein the Chinese herbal medicine extract is prepared by extracting pulp of cornus, dark plum fruit, medicated leaven, ephedra herb, coptis root, datura flower, cassia seeds, wrinkled gianthyssop herb, rhizoma chuanxiong, atratylodes rhizome and cinnamon in a weight ratio of 10:10:15:10:5:10:5:10:5:10:10. The immunoenhancer disclosed by the invention can enhance the reproductive performance and immunity of a pregnant sow and improve the absorption function of intestinal mucosa so as to realize a function of preventing and treating diseases and provide nutrition to promote the growth and development of piglets.

The invention discloses application of quercetin in preparation of a medicine for treating intervertebral disc nucleus pulposus degeneration. Tests prove that quercetin has an obvious effect of protecting nucleus pulposus cells from being damaged. In a rat nucleus pulposus cell apoptosis model of 100microM of TBHP, quercetin can obviously weaken nucleus pulposus cell damage caused by TBHP and maintain normal forms and functions of nucleus pulposus cells; quercetin has low toxicity to nucleus pulposus cells, and almost has no cytotoxicity within 200microM; quercetin can be used for remarkably relieving oxidative stress, degeneration and apoptosis of TBHP-induced nucleus pulposus cells of rats; according to the invention, new application of quercetin is developed, the use value of quercetin is improved, and theoretical support is provided for application of quercetin in preparation of drugs for preventing intervertebral disc degeneration.

The invention relates to a feed capable of improving semen quality of breeder cocks. Sodium butyrate, compound organic trace elements and arginine are added in the formula of the feed so as to synergistically take an effect, so that, the feed is capable of significantly improving semen quality of breeder cocks; and the feed is especially capable of significantly improving semen quality of the breeder cocks in the middle and late growth stages as well as subsequently enhancing reproductive performance of the breeder cocks so as to significantly improve fertilization rate of setting eggs in themiddle and late growth stages. The feed is also capable of prolonging service lives of the breeder cocks as well as reducing investment in breeding cocks to a certain extent, thereby decreasing cost and increasing economic benefits.

A medicine composition for treating renal interstitial fibrosis is disclosed. Effective components of the composition are rosiglitazone or a pharmaceutically acceptable salt thereof and puerarin. Thepharmaceutically acceptable salt of the rosiglitazone is one of rosiglitazone hydrochloride or rosiglitazone maleate or a combination thereof. The composition has significant antifibrosis activity, anti-inflammatory activity and antioxidation stress activity which are superior to those of a single medicine, and can significantly improve renal interstitial fibrosis.

The invention relates to an antibacterial, anti-inflammatory and antioxidant carex meyeriana extract as well as a preparation method and application thereof, and belongs to the field of phytochemistry. The preparation method comprises the following steps of crushing carex meyeriana, adding 50-95% ethanol in an amount which is 8-12 times the volume mass of the carex meyeriana, soaking for 20-60 minutes, carrying out reflux extraction twice for 20-60 minutes each time, filtering, merging filtrates, concentrating and drying to obtain the carex meyeriana extract. The invention discloses application of the antibacterial, anti-inflammatory and antioxidant carex meyeriana extract in preparation of an antibacterial agent, an anti-inflammatory agent or an antioxidant. The antibacterial, anti-inflammatory, antioxidant and carex meyeriana extract disclosed by the invention is derived from cyperaceae Carex Linn. plant carex meyeriana, is an ethanol extract of whole grass of carex meyeriana, and has good antibacterial, anti-inflammatory and antioxidant effects; and the extract has an inhibiting effect on gram-negative bacteria, gram-positive bacteria, fungus typical pathogenic bacteria such asstaphylococcus aureus, escherichia coli, pseudomonas aeruginosa, candida albicans and the like.

The invention relates to application of piceatannol-3'-O-beta-D-glucopyranoside in treatment of small intestine mucosa injury and intestinal inflammatory cell infiltration caused by ischemia reperfusion. Experimental results show that by virtue of the piceatannol-3'-O-beta-D-glucopyranoside, the yield of malondialdehyde (MDA) and the activity of myeloperoxidase (MPO) in intestinal tissues after ischemia / reperfusion can be remarkably reduced, the expression level of tumor necrosis factor-alpha (TNF-alpha) can be reduced, the expression level of endothelial nitric oxide synthase (eNOS) can be increased, the activity of total-superoxide dismutase (T-SOD) can be remarkably improved, and intestinal interstitial hemorrhage and small intestine mucosa defect are relieved. The piceatannol-3'-O-beta-D-glucopyranoside can effectively treat the small intestine ischemia reperfusion injury, and a basis is provided for application of the piceatannol-3'-O-beta-D-glucopyranoside in treatment of small intestine ischemia reperfusion related diseases.

The invention provides an application of lactobacillus rhamnosus LRa05 in preparation of a preparation for improving irritable bowel syndrome. The lactobacillus rhamnosus LRa05 freeze-dried powder provided by the invention has a remarkable curative effect on the irritable bowel syndrome, and can be used for reducing serum endotoxin, reducing proinflammatory factors, increasing the level of anti-inflammatory factors, relieving oxidative stress and adjusting intestinal flora disorder, so that the intestinal flora is healthy. The lactobacillus rhamnosum LRa05 freeze-dried powder product provided by the invention is simple to prepare and suitable for large-scale production, has the characteristics of simple treatment, high remission rate, no toxic or side effect and the like as an auxiliary medicine of IBS, and is relatively high in practicability.

The invention discloses a feed additive for preventing diarrhea in lactating calves and an application method thereof. The present invention uses natural astaxanthin, sodium humate, β-hydroxy-β-methylbutyric acid, glutamine, xylooligosaccharides and Chinese herbal medicine additives (agrimony, honeysuckle, pulsatilla, hawthorn, pomegranate peel, talc, licorice ) to formulate formulas to regulate the balance of gastrointestinal flora in lactating calves, promote the development of gastrointestinal mucosa, reduce the damage caused by pathogens and unfavorable factors, increase the digestion and absorption of nutrients, improve the body's immunity, and relieve the oxidative stress suffered Stress and oxidative damage, improve the anti-stress ability, so as to prevent the occurrence of diarrhea in lactating calves, promote the absorption of feed nutrients, and improve the health level and production performance of calves.

The invention provides a nutrient reinforcing agent for sows at the perinatal period, and belongs to the technical field of raising of sows at the perinatal period. The nutrient reinforcing agent comprises 120-300 parts of isomaltooligosaccharide, 150-250 parts of baking soda, 30-60 parts of VE, 35-65 parts of VC, 0.01-0.03 part of pyrroloquinoline quinone, 175-290 parts of biolys, 12-33 parts ofcalcium formate, 10-20 parts of sorbitol, 1-3 parts of superoxide dismutase, 1-5 parts of selenium yeast and 100-600 parts of bran. The invention further provides a preparation method and applicationof the nutrient reinforcing agent. The nutrient reinforcing agent can relieve oxidative stress of the sows at the perinatal period, the food consumption of the sows at the lactation period can be increased, the heat interval of the sows at the weaning period can be reduced, and the reproductive performance of the sows can be improved.

The invention discloses a preparation method and application of multifunctional pea peptide, and belongs to the technical field of biological medicines.The preparation method of the multifunctional pea peptide comprises the following steps that S1, pea protein feed liquid is prepared; s2, preparation of compound protease: 1) fermentation: inoculating the pea protein material liquid with bacillus natto for fermentation, centrifuging the fermentation liquid, taking the supernatant, and drying to obtain the compound protease; 2) compounding: compounding the compound protease with papain, alkaline protease and flavourzyme to prepare compound protease; s3, performing enzymolysis; s4, performing membrane filtration; and S5, drying. The method is simple in process, short in period, high in yield and low in cost, the prepared pea peptide is high in yield and protein recovery rate, the protein hydrolysate is small in relative molecular mass, the product flavor is good, and the pea peptide product has blood pressure reduction, uric acid reduction and antioxidant activity and is a high-quality pea peptide product.

The present invention provides a premix for preventing and treating fatty liver of dairy cattle in a perinatal period and a related application thereof. The premix simultaneously contains a certain amount of ruminal choline and selenium, creatively utilizes a combination of the two materials to have a double protection function for liver, groundbreakingly realizes a synergic effect of the two materials added in the premix at the same time, is prefect in liver protection, antioxidant and lactating performances of the dairy cattle in the perinatal period, and realizes an one-plus-one-is-greater-than-two effect.

The invention discloses a synthetic method of a eumelanin-like nano contrast agent loaded with a therapeutic drug, which comprises the following steps: firstly, polymerizing metal salt ions as a manganese source and dopamine hydrochloride as precursor molecules to form manganese-eumelanin nano particles, and then loading a targeted drug on the manganese-eumelanin nano particles to obtain the eumelanin-like nano contrast agent. The invention also discloses an application of the eumelanin-like nano contrast agent loaded with the therapeutic drug. The eumelanin-like nano contrast agent provided by the invention has high metal loading capacity and geometric confinement effect, and shows excellent T1-T2 dual-mode MRI contrast enhancement capability; meanwhile, a neuroprotective agent curcumin is loaded on angiopep-2 functionalized and manganese-doped eumelanin nanoparticles to prepare antioxidation and anti-neuroinflammation for targeted diagnosis and treatment of traumatic brain injury and AMEC accumulated at a traumatic part, so that a long-term treatment effect on secondary brain injury is realized; the AMEC well combines a drug carrier with medical imaging, and has good clinical application potential.