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Preparation method and application of bulbus fritillariae cirrhosae extract

A technology of Fritillaria chinensis and extracts, which is applied in the field of preparation of Fritillaria chinensis extracts, can solve the problems of no clear methods and curative effect data for therapeutic applications, and achieve the effect of reducing inflammatory reactions

Pending Publication Date: 2022-02-08
SICHUAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a precious Chinese herbal medicine, Chuan Fritillaria has proven its safety and toxicity, but there is no clear method and curative effect data for the treatment and application of these three diseases. It has great potential and application prospects

Method used

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  • Preparation method and application of bulbus fritillariae cirrhosae extract
  • Preparation method and application of bulbus fritillariae cirrhosae extract
  • Preparation method and application of bulbus fritillariae cirrhosae extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing an extract of Fritillaria sichuanensis, the method comprising:

[0035] Take 6kg of fritillary bulbs and grind them into powder, soak them in 85% ethanol overnight, put them in a large and medium-sized continuous reflux concentration extraction tank, and continuously reflux extraction at 70-80°C for 8 hours, collect the extraction concentrate, and use a rotary evaporator to remove the residual solvent to obtain a concentrated extract , put into a decompression vacuum drying oven, and dry at 50° C. for 24 hours. The Fritillaria de Chuan extract weighed 581.4 g, and the yield of the extract was 9.69%.

experiment example 1

[0036] Determination of Alkaloid Content in Experimental Example 1 Fritillaria sichuan Extract

[0037] The determination of alkaloid content in the Fritillaria sichuan extract prepared in embodiment 1, adopts LC-MS to measure the content of alkaloid:

[0038] Chromatographic conditions of LC-MS: the chromatographic column is Shim-pack XR-ODS (2.2μm, 2.00mm×100mm I.D.), and the column temperature is 40°C. Mobile phase: 0.1% formic acid aqueous solution (A), acetonitrile (B), gradient elution, 0.00→1.00min, B%0.0→15.0; 1.00→3.00min, B%15.0→25.0; 3.00→4.00min, B%25.0 →25.0; 4.00→5.00min, B%25.0→95.0; 5.00→5.50min, B%95.0→95.0; 5.50→5.60min, B%95.0→15.0; 5.60→7.90min, B%15.0→15.0; 8.00min, stop. Flow rate: 0.40mL·min -1 , the injection volume is 5 μL.

[0039] Mass spectrometry conditions: ESI source; positive ion mode monitoring mode, injection voltage is set to 5500V; ion source temperature is 500°C. The atomizing gas (Gas1) is 50.0psi, the heating gas (Gas2) is 50.0psi, t...

experiment example 2

[0041] Preparation of experimental example 2 experimental animal model

[0042] 1. Grouping of experimental animals

[0043] 100 SD rats were reared separately by sex in the animal room of West China School of Pharmacy, Sichuan University for one week, and had free access to water and food to adapt to the environment. One week later, the body weight was weighed and recorded, and divided into blank control group (C), COPD model group (P), low-dose administration group (L), middle-dose administration group ( Group (M) and high-dose administration group (H), 20 rats in each group, half male and half male.

[0044] 2. Establishment of COPD rat disease model and drug treatment

[0045] The rat COPD disease model was established by lipopolysaccharide (LPS) tracheal instillation and fumigation, and the rat COPD disease model was established by 6 weeks as a cycle. On the 1st and 15th days, the rats in the experimental group were anesthetized with 4% chloral hydrate (10mL / kg) intraper...

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Abstract

The invention discloses a preparation method and application of a bulbus fritillariae cirrhosae extract. The method comprises the following steps: pulverizing bulbus fritillariae cirrhosae, soaking the bulbus fritillariae cirrhosae in an alcoholic solution overnight, carrying out continuous reflux extraction at 60-90 DEG C, collecting an extracted concentrated solution, removing a solvent to obtain a concentrated extract, and carrying out reduced-pressure vacuum drying to obtain the bulbus fritillariae cirrhosae extract. Alkaloids in the bulbus fritillariae cirrhosae extract comprise sipeimine, peiminine, peimine, edpetiline, peimine, peimisine, sinpeinine A, yibeinoside A and ebeiedinone. The bulbus fritillariae cirrhosae extract disclosed by the invention can be used as a medicine for treating chronic respiratory system diseases, including chronic obstructive pneumonia, chronic pharyngitis and pulmonary fibrosis, and can activate MAPKs and Nrf2 signal channels.

Description

technical field [0001] The invention relates to the extraction of Fritillaria sichuanensis, in particular to a preparation method and application of Fritillaria sichuanensis extract. Background technique [0002] The pharynx refers to the cavity above the esophagus behind the oral cavity and nasal cavity. It is a common channel for eating and breathing, and is composed of muscles and mucous membranes. The lung is a part of the respiratory system, and its function is to exchange gases. Good lung function is the guarantee for maintaining life. The two are important components of the respiratory tract and are important functional parts of the body for gas exchange. [0003] Chronic obstructive pulmonary disease (COPD for short) is a group of lung diseases characterized by airflow limitation. The progressive development of airflow limitation is related to the enhanced chronic inflammatory response of airways and lungs to noxious particles or gases. Chronic cough, sputum, and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8966A61K31/58A61K31/706A61P11/00A61P11/04A61K125/00
CPCA61K36/8966A61K31/58A61K31/706A61P11/00A61P11/04
Inventor 叶本贵贺喜雷龙
Owner SICHUAN UNIV
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