Traditional Chinese medicine fumigant for treating combustion type lung diseases and preparation method of traditional Chinese medicine fumigant
By preparing a specific formula of combustion Chinese medicine fumigant and fermenting treatment of fermentation broth, the shortcomings of existing Chinese medicine fumigants in the treatment of lung inflammation are solved, significant anti-inflammatory and antioxidant effects are achieved, and the effect of treating lung diseases is improved.
Patent Information
- Application Number
- CN202510237948.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-03
- Publication Date
- 2025-06-17
AI Technical Summary
There are insufficient research and development in the treatment of lung diseases in the existing traditional Chinese medicine fumigants, especially in the effective relieving lung inflammation.
A Chinese medicine fumigant for burning lung disease is proposed. A fumigant with antioxidant and anti-inflammatory effects is prepared by combining a specific formula and a fermentation broth. The fumigant includes medicinal materials such as Gastrodia elata, Ganoderma lucidum, and Fritillaria kebayashi, which are fermented with a fermentation broth to form a fumigant with enhanced medicinal effects.
This fumigant significantly alleviated lung inflammation in the mouse model, reduced the proportion of inflammatory cells and the concentration of inflammatory factors, reduced pulmonary edema, enhanced the antioxidant ability of drug ingredients, and thus improved the therapeutic effect.
Abstract
Description
Technical Field
[0001] The present invention relates to the field of traditional Chinese medicine fumigation agents, and particularly to a traditional Chinese medicine smoke agent for treating combustion-type lung diseases and a preparation method thereof. Background Art
[0002] Fumigation therapy is one of the commonly used external treatment methods in traditional Chinese medicine and an important part of Chinese medicine. Guided by the basic theory of traditional Chinese medicine, it selects Miao folk Chinese herbal medicines and fumigates with the aerosol generated after boiling. By the direct action of the medicinal power and heat on the fumigated part, it can achieve the effects of dilating local blood vessels, promoting blood circulation, warming the blood vessels, removing toxins and killing bacteria, relieving itching, cleaning wounds, reducing swelling and relieving pain, and finally achieving the purposes of treating diseases, preventing diseases and health care.
[0003] In the prior art, there is still room for research and development of traditional Chinese medicine fumigation agents, especially for common lung problems, such as common lung or respiratory inflammations. Summary of the Invention
[0004] In view of the deficiencies of the prior art, the present invention provides a traditional Chinese medicine smoke agent for treating combustion-type lung diseases and a preparation method thereof.
[0005] The object of the present invention can be achieved by the following technical solutions:
[0006] A traditional Chinese medicine smoke agent for treating combustion-type lung diseases, comprising the following raw materials in parts by weight:
[0007] 5 parts of Gastrodia elata, 5 parts of Ganoderma lucidum, 3 parts of Fritillaria cirrhosa, 10 parts of desert cistanche, 5 parts of Chinese chive seeds, 10 parts of Cynomorium songaricum, 10 parts of Lycium ruthenicum, 5 parts of Dendrobium officinale, 10 parts of Platycodon grandiflorum, 10 parts of dried tangerine peel, 10 parts of Exocarpium Citri Grandis, 10 parts of Poria cocos, 20 parts of Taraxacum mongolicum, 10 parts of cucumber seeds, 5 parts of Sargassum, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Morus alba leaves, 5 parts of Glycyrrhiza uralensis, 10 parts of Platycladus orientalis leaves, and 10 parts of Perilla frutescens leaves; and 150 parts of fermentation broth.
[0008] Optionally, it further comprises: 0.1 part of Aquilaria sinensis, 0.5 part of Dalbergia odorifera, 5 parts of Aoki box, 0.1 part of Santalum album, and 10 parts of Pogostemon cablin.
[0009] Optionally, it further comprises: 10 parts of Hippohgae rhamnoides leaves, 5 parts of Osmanthus fragrans, 3 parts of Mentha haplocalyx, 2 parts of Artemisia argyi, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Morus alba leaves, 5 parts of Magnolia liliflora, 10 parts of Paeonia lactiflora, 10 parts of Rosa rugosa, and 10 parts of Paeonia suffruticosa.
[0010] Optionally, the fermentation broth comprises the following components by mass fraction: 0.5% of yeast, 0.2% of white sugar, 0.4% of brown sugar, 0.15% of Jiangxiangxing Baijiu, 0.4% of distiller's yeast, and 46% of water.
[0011] Optionally, a preparation method of a traditional Chinese medicine smoke agent comprises the following steps:
[0012] Weigh the following raw materials by weight parts:
[0013] 5 parts of Gastrodia elata, 5 parts of Ganoderma lucidum, 3 parts of Fritillaria cirrhosa, 10 parts of Cistanche deserticola, 5 parts of Chinese chive seeds, 10 parts of Cynomorium songaricum, 10 parts of Lycium ruthenicum, 5 parts of Dendrobium officinale, 10 parts of Platycodon grandiflorum, 10 parts of Tangerine peel, 10 parts of Exocarpium Citri Grandis, 10 parts of Poria cocos, 20 parts of Taraxacum mongolicum, 10 parts of Cucumber seeds, 5 parts of Sargassum, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Mulberry leaves, 5 parts of Licorice, 10 parts of Biota orientalis and 10 parts of Perilla leaves, and perform crushing treatment;
[0014] Steam in a water bath, stir-fry until yellow at 85°C after drying;
[0015] Add 150 parts by weight of fermentation broth, ferment in a closed container to obtain the traditional Chinese medicine smoke agent.
[0016] Optionally, the raw materials further include: 0.1 part of Aquilaria sinensis, 0.5 part of Dalbergia odorifera, 5 parts of Aoki box, 0.1 part of Santalum album and 10 parts of Pogostemon cablin.
[0017] Optionally, the raw materials further include: 10 parts of Hippohgae rhamnoides leaves, 5 parts of Osmanthus fragrans, 3 parts of Mentha haplocalyx, 2 parts of Artemisia argyi, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Mulberry leaves, 5 parts of Magnolia liliflora, 10 parts of Paeonia lactiflora, 10 parts of Rosa rugosa and 10 parts of Paeonia suffruticosa.
[0018] Optionally, the fermentation broth includes the following mass fraction components: 0.5% of yeast, 0.2% of white sugar, 0.4% of brown sugar, 0.15% of Jiangxiangxing Baijiu, 0.4% of koji and 46% of water.
[0019] Optionally, the raw materials further include β-glucan and / or arabinose.
[0020] Advantages of the present invention:
[0021] The fumigation drug of the present application effectively alleviates the inflammation symptoms of mice with lung inflammation, and improves the antioxidant capacity of the drug components during the fumigation process through polysaccharide components, assisting in enhancing the efficacy of the drug. Specific embodiments
[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Apparently, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.
[0023] Example 1 provides a traditional Chinese medicine smoke agent and its preparation method, including the following steps:
[0024] Weigh the following raw materials quantitatively:
[0025] 5 parts of Gastrodia elata, 5 parts of Ganoderma lucidum, 3 parts of Fritillaria cirrhosa, 10 parts of Cistanche deserticola, 5 parts of Allium tuberosum seeds, 10 parts of Cynomorium songaricum, 10 parts of Lycium ruthenicum, 5 parts of Dendrobium officinale, 10 parts of Platycodon grandiflorum, 10 parts of Citrus reticulata Blanco, 10 parts of Exocarpium Citri Grandis, 10 parts of Poria cocos, 20 parts of Taraxacum mongolicum, 10 parts of Cucumber seeds, 5 parts of Sargassum, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Morus alba leaves, 5 parts of Glycyrrhiza uralensis, 10 parts of Platycladus orientalis leaves and 10 parts of Perilla frutescens leaves; 0.1 part of Aquilaria sinensis, 0.5 part of Dalbergia odorifera, 5 parts of Aucuba japonica, 0.1 part of Santalum album and 10 parts of Pogostemon cablin; 10 parts of Hippohgae rhamnoides leaves, 5 parts of Osmanthus fragrans, 3 parts of Mentha haplocalyx, 2 parts of Artemisia argyi, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Morus alba leaves, 5 parts of Magnolia liliflora Desr., 10 parts of Paeonia lactiflora Pall., 10 parts of Rosa rugosa and 10 parts of Paeonia suffruticosa.
[0026] Mix and crush the above raw materials, steam them in a water bath, and stir-fry them until they turn yellow at 85°C after drying.
[0027] Add 150 parts by weight of the fermentation broth, put it into a fermentation container, and ferment it sealed.
[0028] The fermentation broth includes the following components by mass fraction: 0.5% of yeast, 0.2% of white sugar, 0.4% of brown sugar, 0.15% of Jiangxiangxing Baijiu, 0.4% of distiller's yeast and 46% of water.
[0029] Example 2 provides a traditional Chinese medicine fumigant and its preparation method, including the following steps:
[0030] Weigh the following raw materials quantitatively:
[0031] 5 parts of Gastrodia elata, 5 parts of Ganoderma lucidum, 3 parts of Fritillaria cirrhosa, 10 parts of Cistanche deserticola, 5 parts of Allium tuberosum seeds, 10 parts of Cynomorium songaricum, 10 parts of Lycium ruthenicum, 5 parts of Dendrobium officinale, 10 parts of Platycodon grandiflorum, 10 parts of Citrus reticulata Blanco, 10 parts of Exocarpium Citri Grandis, 10 parts of Poria cocos, 20 parts of Taraxacum mongolicum, 10 parts of Cucumber seeds, 5 parts of Sargassum, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Morus alba leaves, 5 parts of Glycyrrhiza uralensis, 10 parts of Platycladus orientalis leaves and 10 parts of Perilla frutescens leaves; 0.1 part of Aquilaria sinensis, 0.5 part of Dalbergia odorifera, 5 parts of Aucuba japonica, 0.1 part of Santalum album and 10 parts of Pogostemon cablin; 10 parts of Hippohgae rhamnoides leaves, 5 parts of Osmanthus fragrans, 3 parts of Mentha haplocalyx, 2 parts of Artemisia argyi, 5 parts of Angelica sinensis, 5 parts of Panax notoginseng (processed), 10 parts of Isatis indigotica, 10 parts of Morus alba leaves, 5 parts of Magnolia liliflora Desr., 10 parts of Paeonia lactiflora Pall., 10 parts of Rosa rugosa, 10 parts of Paeonia suffruticosa; 5 parts of β-glucan and 5 parts of arabinose.
[0032] Mix and crush the above raw materials, steam them in a water bath, and stir-fry them until they turn yellow at 85°C after drying.
[0033] Add 150 parts by weight of the fermentation broth, put it into a fermentation container, and ferment it sealed.
[0034] The fermentation broth comprises the following components by mass fraction: yeast 0.5%, white sugar 0.2%, brown sugar 0.4%, Maotai-flavor liquor 0.15%, distiller's yeast 0.4%, and water 46%.
[0035] In some embodiments of the present application, the fumigants of the above-mentioned Example 1 and Example 2 are used on a mouse model to test the efficacy of the drug.
[0036] Taking the acute pneumonia model induced by lipopolysaccharide (LPS) as an example, the present application triggers an acute inflammatory response in the lungs by activating the immune system, which is a classic model for studying lung inflammation. The specific steps are as follows:
[0037] Select C57BL / 6 mice aged 8 - 10 weeks, weighing 20 - 25 grams, in good health.
[0038] Prepare a lipopolysaccharide (LPS, concentration 5 mg / kg) solution.
[0039] Through tail vein injection, each mouse is injected with 5 mg / kg LPS; after injection, the inflammatory response induced by LPS occurs.
[0040] Randomly divide the above-mentioned LPS mice into three groups:
[0041] Blank control group: LPS, without any other treatment;
[0042] Drug treatment group 1: LPS + fumigation with the fumigant of Example 1,
[0043] Drug treatment group 2: LPS + fumigation with the fumigant of Example 2.
[0044] The fumigation step includes placing the mice in a fumigation container, heating the fumigation liquid from the bottom of the container and maintaining at 38 degrees, and performing the fumigation operation twice a day for 10 days.
[0045] In the subsequent steps, the treatment effects of the fumigants of Example 1 and Example 2 are evaluated through experimental tests.
[0046] 1 Detection of the number of infiltrating cells in the lungs
[0047] Extract the lung tissue cells of the above three groups of mice, use flow cytometry to classify the immune cells in the lung tissue, and calculate the proportions of different immune cell subsets.
[0048] LPS (blank control group):
[0049] Neutrophils: up to 30%.
[0050] Macrophages: 15%.
[0051] CD4+ T cells: 20%.
[0052] CD8+ T cells: 12%.
[0053] B cells: 5%.
[0054] Drug treatment group 1: LPS + fumigation with Example 1
[0055] Neutrophils: Reduced to 23% (significantly decreased, indicating that the drug has an anti-inflammatory effect).
[0056] Macrophages: Reduced to 11%.
[0057] CD4+ T cells: 15% (slightly lower than the blank control group, indicating the immunomodulatory effect of the drug).
[0058] CD8+ T cells: 8% (decreased, indicating that the drug reduces the T cell-mediated immune response).
[0059] B cells: 5% (no significant change).
[0060] LPS + Example 1 + β-glucan fumigation
[0061] Neutrophils: Reduced to 26% (significantly decreased, indicating that the drug has an anti-inflammatory effect).
[0062] Macrophages: Reduced to 13%.
[0063] CD4+ T cells: 15% (slightly lower than the blank control group, indicating the immunomodulatory effect of the drug).
[0064] CD8+ T cells: 8% (decreased, indicating that the drug reduces the T cell-mediated immune response).
[0065] B cells: 5% (no significant change).
[0066] 2 Detection of cytokine levels
[0067] ELISA was used to detect the concentration changes of major inflammatory factors (such as TNF-α, IL-6, IL-1β, etc.) in lung tissue or serum.
[0068] LPS model group:
[0069] TNF-α: 350 pg / mL, reflecting a strong inflammatory response.
[0070] IL-6: 252 pg / mL.
[0071] IL-1β: 207 pg / mL.
[0072] Drug treatment group 1: LPS + fumigation with Example 1:
[0073] TNF-α: Reduced to 120 pg / mL, showing the anti-inflammatory effect of Drug A.
[0074] IL-6: Reduced to 102 pg / mL.
[0075] IL-1β: Reduced to 80 pg / mL.
[0076] Drug treatment group 2: LPS + Example 1 + β-glucan fumigation:
[0077] TNF-α: Reduced to 98 pg / mL, showing the anti-inflammatory effect of Drug A.
[0078] IL-6: Reduced to 81 pg / mL.
[0079] IL-1β: Reduced to 76 pg / mL.
[0080] 3 Pulmonary edema assessment
[0081] Measure the wet / dry weight ratio of the lungs to evaluate the degree of pulmonary edema.
[0082] Blank control group: Obvious pulmonary edema, wet / dry weight ratio is 5.2.
[0083] Drug treatment group 1: LPS + fumigation with Example 1: Pulmonary edema alleviated, wet / dry weight ratio decreased to 2.8.
[0084] Drug treatment group 2: LPS + Example 1 + β-glucan fumigation: Pulmonary edema alleviated, wet / dry weight ratio decreased to 2.1.
[0085] From the above experimental data, it can be seen that the fumigant of the present application can achieve good therapeutic effects.
[0086] Among them, polysaccharide substances (such as β-glucan, arabinose, mannose, etc.) can enhance the antioxidant capacity of herbal components through several mechanisms, mainly through the following principles:
[0087] Polysaccharide substances have abundant hydrophilic functional groups such as hydroxyl (-OH) and alcohol (-OH). These functional groups can form complexes with the active ingredients in herbs (such as polysaccharides, flavonoids, terpene compounds, etc.). This complex can stabilize the active ingredients in herbs, reduce their oxidative degradation, and thus maintain their antioxidant activity. Especially for water-soluble active ingredients, polysaccharides can help them remain stable and reduce oxidation reactions. Enhance the efficacy of drugs, especially in fumigation drugs.
[0088] β-glucan has very strong hydrophilicity and can form complexes with some water-soluble drugs (such as Ganoderma lucidum polysaccharides, the active ingredients in Fritillaria cirrhosa, etc.), preventing their degradation or oxidation in the body and enhancing their antioxidant effects.
[0089] β-glucan can indirectly increase the activity of antioxidant enzymes by activating immune cells (such as macrophages, T cells, B cells, etc.) in the immune system. This is because when the immune system responds to oxidative stress and inflammatory responses in the body, it will activate a series of antioxidant enzyme systems, such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase.
[0090] The specific principles include: β-glucan binds to receptors in the immune system (such as Dectin-1 receptor, Toll-like receptor, etc. in white blood cells), activating immune cells such as macrophages and dendritic cells. After being activated, these immune cells will secrete cytokines and immunomodulatory substances, promoting the expression of antioxidant enzymes, thereby enhancing the antioxidant defense in the body.
[0091] Studies have found that the immunomodulatory effect of β-glucan is closely related to the antioxidant response and can help scavenge free radicals in the body and reduce oxidative damage.
[0092] β-glucan can enhance the ability of macrophages to scavenge free radicals, indirectly increase the activity of antioxidant enzymes in the body, and thus enhance the antioxidant effects of the active ingredients in herbs (such as Ganoderma lucidum, Fritillaria cirrhosa, etc.).
[0093] Polysaccharide substances reduce oxidative damage by promoting the cell self-repair process. For example, β-glucan not only increases the activity of antioxidant enzymes through the immune system but also helps damaged cells in the body recover their functions by promoting cell repair and regeneration, indirectly increasing the antioxidant capacity.
[0094] In the description of this specification, the descriptions referring to terms such as "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.
[0095] The foregoing has shown and described the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and what is described in the above embodiments and the specification is only to illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have various changes and improvements, and these changes and improvements fall within the scope of the present invention claimed.
Claims
1. A Chinese medicinal fumigant for treating burning lung diseases, characterized in that: It includes the following raw materials in parts by weight: 5 parts of Gastrodia elata, 5 parts of Ganoderma lucidum, 3 parts of Fritillaria cirrhosa, 10 parts of Herba Cistanches deserticola, 5 parts of Allium tuberosum seeds, 10 parts of Cynomorium songaricum, 10 parts of Lycium barbarum, 5 parts of Dendrobium officinale, 10 parts of Platycodon grandiflorum, 10 parts of Tangerine peel, 10 parts of Citrus aurantium, 10 parts of Poria cocos, 20 parts of Taraxacum herb, 10 parts of Cucumis sativus seeds, 5 parts of Sargassum, 5 parts of Angelica sinensis, 5 parts of Notoginseng prepared, 10 parts of Radix Isatidis, 10 parts of Morus alba, 5 parts of Licorice root, 10 parts of Pleurotus ostreatus leaves and 10 parts of Perilla leaves; and 150 parts of fermented liquid.
2. The Chinese medicinal fumigant for burning lung diseases according to claim 1, characterized in that: Also includes: 0.1 parts of agarwood, 0.5 parts of dalbergia wood, 5 parts of green wood box, 0.1 parts of sandalwood and 10 parts of patchouli.
3. The Chinese medicinal fumigant for burning lung diseases according to claim 1, characterized in that: Also includes: 10 parts of seabuckthorn leaves, 5 parts of osmanthus flowers, 3 parts of mints, 2 parts of wormwood leaves, 5 parts of angelica sinensis, 5 parts of prepared panax notoginseng, 10 parts of isatis indigotica roots, 10 parts of mulberry leaves, 5 parts of magnolia flowers, 10 parts of white peony flowers, 10 parts of rose flowers, and 10 parts of peony flowers.
4. The Chinese medicinal fumigant for burning lung diseases according to claim 1, characterized in that: The fermentation liquid comprises the following components by mass fraction: 0.5% yeast, 0.2% white sugar, 0.4% brown sugar, 0.15% sauce-flavor liquor, 0.4% koji and 46% water.
5. The Chinese medicinal fumigant for burning lung diseases according to claim 1, characterized in that: Also included are beta-glucan and / or arabinose.
6. A method for preparing a Chinese medicine fumigant, characterized in that: The following steps are involved: Weigh the following raw materials in parts by weight: 5 parts of Gastrodia elata, 5 parts of Ganoderma lucidum, 3 parts of Fritillaria cirrhosa, 10 parts of Herba Cistanches deserticola, 5 parts of Allium tuberosum seeds, 10 parts of Cynomorium songaricum, 10 parts of Lycium barbarum, 5 parts of Dendrobium officinale, 10 parts of Platycodon grandiflorum, 10 parts of Tangerine peel, 10 parts of Citrus aurantium, 10 parts of Poria cocos, 20 parts of Taraxacum herb, 10 parts of Cucumis sativus seeds, 5 parts of Sargassum, 5 parts of Angelica sinensis, 5 parts of Notoginseng prepared, 10 parts of Radix Isatidis, 10 parts of Folium Mori, 5 parts of Licorice, 10 parts of Folium Orientalis and 10 parts of Folium Perillae are crushed; Steam it in water, dry it and fry it at 85 degrees until it turns yellow; 150 parts by weight of fermentation liquid is added and fermented in a closed container to obtain the traditional Chinese medicine fumigant.
7. The method for preparing the Chinese medicinal fumigant according to claim 1, characterized in that: The raw materials also include: 0.1 parts of agarwood, 0.5 parts of dalbergia wood, 5 parts of green wood box, 0.1 parts of sandalwood and 10 parts of patchouli.
8. The method for preparing the Chinese medicinal fumigant according to claim 1, characterized in that: The raw materials also include: 10 parts of seabuckthorn leaves, 5 parts of osmanthus, 3 parts of mint, 2 parts of wormwood, 5 parts of angelica, 5 parts of cooked Panax notoginseng, 10 parts of isatis root, 10 parts of mulberry leaves, 5 parts of magnolia flowers, 10 parts of white peony flowers, 10 parts of rose flowers, and 10 parts of peony flowers.
9. The method for preparing the Chinese medicinal fumigant according to claim 1, characterized in that: The fermentation liquid comprises the following components by mass fraction: 0.5% yeast, 0.2% white sugar, 0.4% brown sugar, 0.15% sauce-flavor liquor, 0.4% koji and 46% water.
10. The method for preparing the Chinese medicinal fumigant according to claim 1, characterized in that: The raw material also includes β-glucan and / or arabinose.