Chinese angelica traditional Chinese medicine decoction pieces and processing method thereof

Abstract

The invention discloses Chinese angelica traditional Chinese medicine decoction pieces and a processing method thereof, and belongs to the technical field of traditional Chinese medicine processing. According to the invention, a bio-based eutectic solvent system is adopted, L-proline and syringic acid form a stable complexing structure through pi-pi accumulation and hydrogen-bond interaction, and the dissolution rate of active ingredients is improved by cooperating with the conjugation effect of hydrophobic groups and benzene rings of gamma-nonanolactone; nano cellulose crystals pretreated by sulfuric acid are introduced, surface hydroxyl groups of the nano cellulose crystals and DES components form a hydrogen bond network, a three-dimensional thermal stable structure is constructed, active components are effectively wrapped, and high-temperature degradation is prevented; the low surface tension of the gamma-nonanolactone is utilized to quickly permeate into the cell walls of the medicinal materials, and the magnetic eddy shear force is combined to destroy the fiber structure and accelerate the component release; trehalose is adopted as a freeze-drying protective agent, oil phase agglomeration is inhibited by forming an amorphous glassy state, and the structural integrity of thermosensitive components is protected by hydroxyl hydrogen bonds. No organic solvent is left, medicine residues are subjected to DES secondary extraction and then cooperatively processed with fresh angelica sinensis, and efficient recycling of resources is achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to an Angelica sinensis traditional Chinese medicine slice and a processing method thereof. Background Art

[0002] Angelica sinensis is the dried root of the plant Angelica sinensis in the Umbelliferae family. It is a commonly used traditional Chinese medicine for enriching blood and promoting blood circulation in clinical practice. Its active ingredients include volatile oils (such as ligustilide, n-butylidenephthalide, etc.), organic acids (ferulic acid), polysaccharides, and amino acids, etc., and it is widely used in the fields of gynecological diseases, cardiovascular diseases, and immunomodulation. The traditional processing methods of Angelica sinensis slices mainly include processes such as stir-frying with wine and honey roasting, and the medicinal effects are enhanced through the synergistic effect of adjuvants.

[0003] The existing technologies have the following technical problems: The traditional high-temperature frying process is prone to cause the decomposition of heat-sensitive components such as volatile oils, resulting in a decrease in the content of active ingredients in the slices; the medicinal residues generated during the processing are not resourcefully utilized, causing waste of medicinal materials; the traditional solvent extraction method mostly uses organic solvents such as ethanol, and there is a risk of solvent residues; the dissolution rate of active ingredients is low, and it is difficult to meet the requirements of modern traditional Chinese medicine preparations for the content of active ingredients.

[0004] Deep eutectic solvents (DES) are a new type of green solvent system. They form deep eutectic mixtures through the combination of hydrogen bond donors and acceptors, and have advantages such as strong dissolution ability, high designability, and good biocompatibility. Existing research shows that DES has broad application prospects in the field of natural product extraction, but its application in the processing of Angelica sinensis has not been reported yet. Summary of the Invention

[0005] In view of the above situation, in order to overcome the defects of the existing technologies, the present invention optimizes the extraction process and solvent system to achieve the efficient extraction and retention of the active ingredients of Angelica sinensis. At the same time, the medicinal residues are resourcefully utilized, the utilization rate of medicinal materials is improved, the production cost is reduced, and the technical problems of loss of heat-sensitive components, solvent residues, and resource waste in the traditional processing technology are solved.

[0006] In order to achieve the above object, the following technical solutions are adopted: The present invention provides an Angelica sinensis traditional Chinese medicine slice and a processing method thereof, including the following steps: S1. Mix L-proline and syringic acid in a molar ratio of 1:4 - 10, stir at 60 °C for 30 min to form a basic DES solution, add 5% by volume of γ-nonalactone and 0.3% by mass of nanocellulose crystals, and obtain a multi-component DES solution through ultrasonic-assisted dispersion; S2. Mix the Angelica sinensis medicinal residues and the multi-component DES solution in a mass ratio of 1:10 - 15, and implement the following programmed temperature extraction: Raise the temperature at a heating rate of 2 °C / min to 55 °C, and maintain it at 55 °C for 15 min, Then heat up to 65°C and maintain for 50 min, while starting magnetic eddy stirring at a rotation speed of 300 - 500 rpm; Cool to 25°C at a rate of 5°C / min; Centrifuge at 4000 - 6000 rpm for 10 min and then perform cross-flow filtration through a 0.22 μm ceramic membrane; S3. Add trehalose accounting for 1 - 5% of the filtrate volume to the filtrate obtained in step S2, then perform ultrasonic degassing treatment, centrifugally concentrate the filtrate, then stir magnetically at 300 rpm for 10 min and perform ultrasonic degassing for 5 min, and collect the extract by freeze-drying; S4. Wash fresh Angelica sinensis, soak it in brine for 15 - 20 min, then wash it with purified water and dry it, slice it, add honey accounting for 15 - 25% of the mass of fresh Angelica sinensis, 10 - 20% of yellow rice wine, and add the freeze-dried extract according to the mass ratio of freeze-dried extract to fresh Angelica sinensis of 1:12 - 15, soak for 15 - 20 h, then take out the Angelica sinensis slices and perform supercritical CO2 flash drying to obtain the Angelica sinensis Chinese medicinal slices.

[0007] Furthermore, in step S1, the nanocrystalline cellulose is pretreated with sulfuric acid. The pretreatment method is: mix the nanocrystalline cellulose with a 2 mol / L sulfuric acid solution at a mass ratio of 1:20 - 30, hydrolyze at 50°C for 30 - 50 min. After hydrolysis, centrifuge at 8000 rpm for 10 min, discard the supernatant, wash repeatedly with deionized water until neutral, and reserve after freeze-drying.

[0008] Furthermore, in step S1, the power of ultrasonic-assisted dispersion is 200 - 500 W and the time is 30 - 50 min.

[0009] Furthermore, the conditions for centrifugal concentration of the filtrate in step S3 are: rotation speed 10000 - 15000 rpm, time 20 - 40 min, temperature 4 - 10°C, and concentrate to a solid content of 15 - 20%.

[0010] Furthermore, the power of ultrasonic degassing treatment in step S3 is 200 - 500 W and the time is 5 - 10 min.

[0011] Furthermore, the pre-freezing conditions for freeze-drying in step S3 are: program-cool to -50°C, maintain for 4 h, and the liquid height in the vial ≤ 2 cm.

[0012] Furthermore, the vacuum freeze-drying parameters in step S3 are: Sublimation stage: temperature -30°C, vacuum degree 10 Pa, time 12 - 16 h; Analysis stage: temperature 20°C, vacuum degree 10 Pa, time 8 - 12 h.

[0013] Further, the post-treatment of freeze-drying in step S3 includes: cryogenic freezing and grinding at a frequency of 20 Hz for 3 - 10 min, passing through an 80-mesh sieve, and storing at -20°C after nitrogen filling and sealing.

[0014] Further, the supercritical CO2 drying parameters in step S4 are: pressure 20 - 50 MPa, temperature 30 - 40°C, CO2 flow rate 20 L / h, and drying time 30 - 50 min.

[0015] The beneficial effects of the present invention are as follows: (1) The contents of ferulic acid, ligustilide, and volatile oil in the processed Angelica sinensis Chinese herbal pieces prepared by the present invention are all higher than those of the traditional process. The present invention further processes the produced Angelica sinensis medicinal residues, extracts the active ingredients from the Angelica sinensis medicinal residues through DES solution, and cooperates with fresh Angelica sinensis for processing, realizing the resource utilization of medicinal materials. The components of DES are all bio-based raw materials, and the used γ-nonalactone and nanocellulose crystals are natural extracts, and there is no organic solvent residue in the whole extraction process.

[0016] (2) In the present invention, the DES solvent formed by L-proline and syringic acid forms stable complexes with active ingredients such as ligustilide and ferulic acid in Angelica sinensis through π-π stacking and hydrogen bond interactions, improving the dissolution rate of the active ingredients. The hydrophobic group of γ-nonalactone forms hydrophobic interactions with the phthalide structure of ligustilide, and at the same time its lactone ring forms a conjugated system with the benzene ring of syringic acid, further enhancing the dissolution ability.

[0017] (3) The surface of the nanocellulose crystals pretreated with sulfuric acid is rich in hydroxyl groups, which can form hydrogen bond crosslinks with L-proline and syringic acid in DES, increasing the thermal decomposition temperature of DES and significantly improving the system stability. The three-dimensional network structure formed by the nanocellulose crystals can effectively encapsulate the active ingredients of Angelica sinensis and prevent their decomposition at high temperatures.

[0018] (4) Due to the low surface tension of γ-nonalactone, it can quickly penetrate the cell wall of the medicinal materials. Combining with the shear force generated by magnetic vortex stirring, it can effectively destroy the fiber structure of the Angelica sinensis medicinal materials and improve the release rate of the active ingredients.

[0019] (5) The present invention adds trehalose as a freeze-drying protectant, which can form an amorphous glass state during the freeze-drying process, not only inhibiting the aggregation of oily components but also forming hydrogen bonds with the active ingredients through hydroxyl groups to protect the structural integrity of thermosensitive components such as ligustilide. Description of the Drawings

[0020] Figure 1 It is the determination result of the ferulic acid extraction rate of Examples 1 - 5 and Comparative Examples 1 - 2 of an Angelica sinensis Chinese herbal piece and its processing method of the present invention; Figure 2Determination results of the extraction rate of ligustilide in Examples 1-5 and Comparative Examples 1-2 of an Angelica sinensis traditional Chinese medicine decoction piece and its processing method according to the present invention; Figure 3 Determination results of the ferulic acid content in the decoction pieces processed in Examples 1-8 and Comparative Examples 1-3 of an Angelica sinensis traditional Chinese medicine decoction piece and its processing method according to the present invention; Figure 4 Determination results of the ligustilide content in the decoction pieces processed in Examples 1-8 and Comparative Examples 1-3 of an Angelica sinensis traditional Chinese medicine decoction piece and its processing method according to the present invention; Figure 5 Determination results of the volatile oil content in the decoction pieces processed in Examples 1-8 and Comparative Examples 1-3 of an Angelica sinensis traditional Chinese medicine decoction piece and its processing method according to the present invention.

[0021] The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention, and do not constitute a limitation to the present invention. Detailed implementation manners

[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0023] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the present invention. The preferred implementation methods and materials described herein are only for illustrative purposes and do not limit the content of this application.

[0024] The experimental methods in the following embodiments are all conventional methods unless otherwise specified. The test materials used in the following embodiments are all obtained from commercial channels unless otherwise specified. Embodiment

[0025] An Angelica sinensis traditional Chinese medicine decoction piece and its processing method include the following steps: S1. Mix L-proline and syringic acid in a molar ratio of 1:10, stir at 60°C for 30 min to form a basic DES solution, add 5% by volume of γ-nonalactone and 0.3% by mass of nanocellulose crystals, and perform ultrasonic-assisted dispersion (power 500 W, time 50 min) to obtain a multi-component DES solution; S2. Mix the Angelica sinensis residue and the multi-component DES solution in a mass ratio of 1:15, and perform the following programmed temperature extraction: Heat at a rate of 2°C / min to 55°C and hold at 55°C for 15 min. Then heat to 65°C and maintain for 50 min while starting magnetic eddy stirring (rotation speed 500 rpm). Cool to 25°C at a rate of 5°C / min. Centrifuge at 6000 rpm for 10 min and then perform cross-flow filtration through a 0.22-μm ceramic membrane. S3. Add trehalose at 5% of the filtrate volume to the filtrate obtained in step S2, then perform ultrasonic degassing treatment (power 500 W, time 10 min), subject the filtrate to centrifugal concentration (rotation speed 15000 rpm, time 40 min, temperature 10°C, concentrate to a solid content of 20%), then stir magnetically at 300 rpm for 10 min and then perform ultrasonic degassing for 5 min, and collect the extract by freeze-drying. Freeze-drying parameters: Program the temperature to drop to -50°C, maintain for 4 h, and the filling height of the vials in the ampoule ≤ 2 cm. Sublimation stage: Temperature -30°C, vacuum degree 10 Pa, time 16 h. Analysis stage: Temperature 20°C, vacuum degree 10 Pa, time 12 h. Post-treatment after freeze-drying: Grind by liquid nitrogen freezing (frequency 20 Hz, time 10 min), pass through an 80-mesh sieve, seal with nitrogen filling and store at -20°C. S4. Wash fresh Angelica sinensis, soak in brine for 20 min, then wash with pure water and dry, cut into slices, add honey at 25% of the mass of fresh Angelica sinensis, yellow rice wine at 20%, and add the freeze-dried extract in a mass ratio of freeze-dried extract to fresh Angelica sinensis of 1:15, soak for 20 h, then take out the Angelica sinensis slices and perform supercritical CO2 flash drying (pressure 50 MPa, temperature 40°C, CO2 flow rate 20 L / h, drying time 50 min) to obtain the Angelica sinensis Chinese herbal pieces. Example

[0026] A kind of Angelica sinensis Chinese herbal pieces and its processing method, comprising the following steps: S1. Mix L-proline and syringic acid in a molar ratio of 1:4, stir at 60°C for 30 min to form a basic DES solution, add γ-nonalactone with a volume fraction of 5% and nanocellulose crystals with a mass fraction of 0.3%, and perform ultrasonic-assisted dispersion (power 200 W, time 30 min) to obtain a multi-component DES solution. S2. Mix Angelica sinensis residue and the multi-component DES solution in a mass ratio of 1:10, and perform the following programmed temperature extraction: Heat at a rate of 2°C / min to 55°C and hold at 55°C for 15 min. Then, heat it up to 65 °C and maintain for 50 min, while starting magnetic eddy stirring (rotation speed 300 rpm); Cool it to 25 °C at a rate of 5 °C / min; Centrifuge at 4000 rpm for 10 min and then perform cross-flow filtration through a 0.22 μm ceramic membrane; S3. Add trehalose accounting for 1% of the filtrate volume to the filtrate obtained in step S2, then perform ultrasonic degassing treatment (power 200 W, time 5 min), centrifuge and concentrate the filtrate (rotation speed 10000 rpm, time 20 min, temperature 4 °C, concentrate to a solid content of 15%), then stir magnetically at 300 rpm for 10 min and then perform ultrasonic degassing for 5 min, and collect the extract by freeze-drying; Freeze-drying parameters: Program the temperature to drop to -50 °C, maintain for 4 h, and the liquid filling height in the vial ≤ 2 cm; Sublimation stage: Temperature -30 °C, vacuum degree 10 Pa, time 12 h; Analysis stage: Temperature 20 °C, vacuum degree 10 Pa, time 8 h; Post-treatment after freeze-drying: Grind with liquid nitrogen (frequency 20 Hz, time 3 min), pass through an 80-mesh sieve, seal with nitrogen and store at -20 °C; S4. Wash the fresh Angelica sinensis, soak it in salt water for 15 min, then wash it with purified water and dry it, slice it, add honey accounting for 15% of the mass of the fresh Angelica sinensis, 10% yellow rice wine to the Angelica sinensis slices, and add the freeze-dried extract according to the mass ratio of freeze-dried extract to fresh Angelica sinensis of 1:12, soak for 15 h, then take out the Angelica sinensis slices and perform supercritical CO2 flash drying (pressure 20 MPa, temperature 30 °C, CO2 flow rate 20 L / h, drying time 30 min) to obtain the Angelica sinensis traditional Chinese medicine decoction pieces. Example

[0027] An Angelica sinensis traditional Chinese medicine decoction piece and its processing method, comprising the following steps: S1. Mix L-proline and syringic acid according to a molar ratio of 1:7, stir at 60 °C for 30 min to form a basic DES solution, add 5% volume fraction of γ-nonalactone and 0.3% mass fraction of nanocrystalline cellulose, and obtain a multi-component DES solution through ultrasonic-assisted dispersion (power 350 W, time 40 min); S2. Mix the Angelica sinensis residue and the multi-component DES solution according to a mass ratio of 1:12, and perform the following program-controlled temperature extraction: Heat it up at a rate of 2 °C / min to 55 °C and maintain at 55 °C for 15 min, Then heat it up to 65 °C and maintain for 50 min, while starting magnetic eddy stirring (rotation speed 400 rpm); Cool it to 25 °C at a rate of 5 °C / min; Centrifuge at 5000 rpm for 10 min and then perform cross-flow filtration through a 0.22 μm ceramic membrane; S3. Add trehalose at 3% of the filtrate volume to the filtrate obtained in step S2, then perform ultrasonic degassing treatment (power 350 W, time 8 min), centrifuge and concentrate the filtrate (rotation speed 12500 rpm, time 30 min, temperature 7 °C, concentrate to a solid content of 17%), then stir magnetically at 300 rpm for 10 min and then perform ultrasonic degassing for 5 min, and collect the extract by freeze-drying; Freeze-drying parameters: Program the temperature to drop to -50 °C and maintain for 4 h, the liquid height in the vial ≤ 2 cm; Sublimation stage: Temperature -30 °C, vacuum degree 10 Pa, time 14 h; Analysis stage: Temperature 20 °C, vacuum degree 10 Pa, time 10 h; Post-treatment after freeze-drying: Grind by liquid nitrogen freezing (frequency 20 Hz, time 7 min), pass through an 80-mesh sieve, seal with nitrogen filling and store at -20 °C; S4. Wash fresh Angelica sinensis, soak it in brine for 17 min, then wash it with pure water, dry it, slice it, add honey at 20% of the mass of fresh Angelica sinensis, 15% of yellow rice wine, and add freeze-dried extract according to the mass ratio of freeze-dried extract to fresh Angelica sinensis of 1:13, soak for 17.5 h, then take out the Angelica sinensis slices and perform supercritical CO2 flash drying (pressure 35 MPa, temperature 35 °C, CO2 flow rate 20 L / h, drying time 40 min) to obtain the Angelica sinensis Chinese herbal pieces. Example

[0028] The difference between this example and Example 3 is that the molar ratio of L-proline to syringic acid in step S1 is 1:6, and the rest are the same as in Example 3. Example

[0029] The difference between this example and Example 3 is that the addition amount of trehalose in step S3 is 3%, and the rest are the same as in Example 3. Example

[0030] The difference between this example and Example 3 is that the proportions of honey and yellow rice wine in step S4 accounting for the mass of fresh Angelica sinensis are 15% and 20% respectively, and the rest are the same as in Example 3. Example

[0031] The difference between this example and Example 3 is that the proportions of honey and yellow rice wine in step S4 accounting for the mass of fresh Angelica sinensis are 25% and 10% respectively, and the rest are the same as in Example 3. Example

[0032] The difference between this example and Example 3 is that the supercritical CO2 drying parameters in S4 are a pressure of 35 MPa, a temperature of 35 °C, and a time of 40 min, and the rest are the same as in Example 3.

[0033] Comparative Example 1 In this comparative example, the traditional impregnation extraction method was used to replace the extraction method in steps S1 - S2 to extract the active ingredients from Angelica sinensis. The specific method was as follows: Angelica sinensis was added to a stoppered conical flask and added to 70% ethanol with a mass 10 times that of Angelica sinensis. After shaking well and standing still, after impregnation for 15 days, then freeze-drying and collecting were carried out according to step S3, and the rest of the steps and ratios were the same as in Example 3.

[0034] Comparative Example 2 In this comparative example, the traditional reflux extraction method was used to replace the extraction method in steps S1 - S2 to extract the active ingredients from Angelica sinensis. The specific method was as follows: Angelica sinensis was added to 70% ethanol with a mass 5 times that of Angelica sinensis and refluxed twice for 1.5 h each time. The two extraction solutions were collected and filtered, and then freeze-drying and collecting were carried out according to step S3, and the rest of the steps and ratios were the same as in Example 3.

[0035] Comparative Example 3 In this comparative example, trehalose with a volume of 1 - 5% of the filtrate volume was not added in step S3, and the rest were the same as in Example 3.

[0036] Test Example 1 Determination of the extraction rates of ferulic acid and ligustilide The solutions extracted in steps S3 of Examples 1 - 5 and Comparative Examples 1 - 2 were filtered through a 0.45 μm microporous filter membrane, and then 20 μL was precisely pipetted from each solution and injected into the chromatograph. After that, the contents of ferulic acid and ligustilide were calculated according to the standard curve, and the extraction rates of ferulic acid and ligustilide were calculated. Each group was repeated 3 times, and the measurement results are shown in Figure 1-2 .

[0037] The chromatographic conditions were as follows: the chromatographic column was an octadecylsilyl silica gel chromatographic column, the mobile phase was acetonitrile: 0.085% phosphoric acid solution = 17:83, the detection wavelength was 316 nm, the column temperature was 35 °C, and the flow rate was 1 mL / min.

[0038] From Figure 1 and Figure 2 the data, it can be seen that in the examples, the multi-component DES extraction system (L-proline / syringic acid + γ-nonalactone + nanocellulose crystals) was used, and the extraction rates of ferulic acid and ligustilide were significantly higher than those of traditional ethanol impregnation (Comparative Example 1) and reflux extraction (Comparative Example 2). This is mainly because of the following effects: Hydrogen bond network and hydrophobic interaction. An efficient solvent system was formed through π-π stacking and hydrogen bonding between L-proline and syringic acid, which combined with the phenolic hydroxyl group of ferulic acid and the phthalide structure of ligustilide, enhancing the dissolution ability; Permeability of γ-nonalactone: Its low surface tension promotes the rapid penetration of the solvent into the cell walls of medicinal residues, releasing lipophilic components; Protective effect of nanocrystalline cellulose: The surface hydroxyl groups of nanocrystalline cellulose pretreated with sulfuric acid crosslink with the components of DES to form a three-dimensional network structure, encapsulating thermosensitive components and reducing decomposition during the high-temperature stage.

[0039] However, ethanol impregnation (Comparative Example 1) has low efficiency and a high risk of organic solvent residues; ethanol reflux (Comparative Example 2) shortens the time, but high temperatures easily cause partial decomposition of ferulic acid and ligustilide, resulting in a decrease in the extraction rate.

[0040] Test Example 2 Quality evaluation of traditional Chinese medicine decoction pieces The contents of ferulic acid, ligustilide, and total volatile oil in the decoction pieces prepared in Examples 1-8 and Comparative Examples 1-3 were analyzed by chromatography, and the measurement results are shown in Figures 3-5 .

[0041] It can be seen from Figures 3-5 that the contents of ferulic acid, ligustilide, and total volatile oil in the decoction pieces of Examples 1-8 are all higher than those of Comparative Examples 1-3, and the contents of each substance in Comparative Example 3 are the lowest. This is because trehalose forms an amorphous glassy state, inhibits the oxidation and aggregation of oily components during the freeze-drying process, and its hydroxyl groups form hydrogen bonds with active components to maintain the integrity of the molecular structure; at the same time, in the present invention, supercritical CO2 flash drying is used to quickly remove moisture at low temperature, avoiding the loss of volatile oil caused by traditional high-temperature drying.

[0042] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

[0043] The above description of the present invention and its embodiments is not restrictive. What is shown in the drawings is only one of the embodiments of the present invention, and the actual application is not limited thereto. Generally speaking, if those of ordinary skill in the art are inspired by it and design similar methods and embodiments without creative efforts without departing from the purpose of the present invention, they should all fall within the protection scope of the present invention.

Claims

1. An Angelica sinensis traditional Chinese medicine cut slice and its processing method, characterized in that: It includes the following steps: S1. Mix L-proline and syringic acid at a molar ratio of 1:4 - 10, stir at 60 °C for 30 min to form a basic DES solution, add γ-nonalactone with a volume fraction of 5% and nanocellulose crystals with a mass fraction of 0.3%, and obtain a multi-component DES solution through ultrasonic-assisted dispersion; S2. Mix Angelica sinensis residue and the multi-component DES solution at a mass ratio of 1:10, and implement the following program-controlled temperature extraction: Heat up at a heating rate of 2 °C / min to 55 °C, and maintain at 55 °C for 15 min, then heat up to 65 °C and maintain for 50 min, while turning on magnetic vortex stirring with a rotation speed of 300 - 500 rpm; Cool to 25 °C at a rate of 5 °C / min; Centrifuge at 4000 - 6000 rpm for 10 min and then perform cross-flow filtration through a 0.22 μm ceramic membrane; S3. Add trehalose with a volume of 1 - 5% of the filtrate volume to the filtrate obtained in step S2, then perform ultrasonic degassing treatment, centrifuge and concentrate the filtrate, then stir magnetically at 300 rpm for 10 min and then perform ultrasonic degassing for 5 min, and collect the extract through freeze-drying; S4. Wash fresh Angelica sinensis, soak it in brine for 15 - 20 min, then wash it with pure water and dry it, slice it, add honey accounting for 15 - 25% of the mass of fresh Angelica sinensis, 10 - 20% of yellow rice wine, and add the freeze-dried extract according to a mass ratio of freeze-dried extract to fresh Angelica sinensis of 1:12 - 15, soak for 15 - 20 h, then take out the Angelica sinensis slices and perform supercritical CO2 instantaneous drying to obtain the Angelica sinensis traditional Chinese medicine slices.

2. The angelica traditional Chinese medicine cut pieces according to claim 1 and its processing method are characterized in that: In step S1, the nanocellulose crystals are pretreated with sulfuric acid, and the pretreatment method is: mix the nanocellulose crystals and 2 mol / L sulfuric acid solution at a mass ratio of 1:20 - 30, hydrolyze at 50 °C for 30 - 50 min, after hydrolysis, centrifuge at 8000 rpm for 10 min, discard the supernatant, wash repeatedly with deionized water until neutral, and freeze-dry for later use.

3. The angelica traditional Chinese medicine cut pieces according to claim 2 and its processing method are characterized in that: In step S1, the power of ultrasonic-assisted dispersion is 200 - 500 W, and the time is 30 - 50 min.

4. The Angelica sinensis traditional Chinese medicine cut crude drug and its processing method according to claim 3, characterized in that: In step S3, the conditions for centrifugal concentration of the filtrate are: rotation speed 10000 - 15000 rpm, time 20 - 40 min, temperature 4 - 10 °C, and concentrate to a solid content of 15 - 20%.

5. The Angelica sinensis traditional Chinese medicine cut crude drug according to claim 4 and its processing method, characterized in that: In step S3, the power of ultrasonic degassing treatment is 200 - 500 W, and the time is 5 - 10 min.

6. The Angelica sinensis traditional Chinese medicine cut crude drug according to claim 5 and its processing method, characterized in that: The pre-freezing conditions for freeze-drying in step S3 are: program-cool to -50 °C, maintain for 4 h, and the liquid height in the vial ≤ 2 cm.

7. The Angelica sinensis traditional Chinese medicine cut crude drug according to claim 6 and its processing method are characterized in that: The vacuum freeze-drying parameters in step S3 are: Sublimation stage: temperature -30 °C, vacuum degree 10 Pa, time 12 - 16 h; Analysis stage: temperature 20 °C, vacuum degree 10 Pa, time 8 - 12 h.

8. A Chinese angelica traditional Chinese medicine cut slice and its processing method according to claim 7, characterized in that: The post-treatment after freeze-drying in step S3 includes: liquid nitrogen freezing and grinding, frequency 20 Hz, time 3 - 10 min, pass through an 80-mesh sieve, seal with nitrogen filling and store at -20 °C.

9. The Angelica sinensis traditional Chinese medicine cut crude drug according to claim 8 and its processing method, characterized in that: The supercritical CO2 drying parameters in step S4 are as follows: pressure 20 - 50 MPa, temperature 30 - 40 °C, CO2 flow rate 20 L / h, and drying time 30 - 50 min.

10. A Chinese herbal medicine slice of Angelica sinensis, characterized in that: Prepared by the preparation method according to any one of claims 1 - 9.

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