Antioxidant peptide and preparation method and application thereof
A technology of antioxidant peptides and antioxidant activity, applied in the biological field, can solve problems such as few studies on functional characteristics, and achieve high-value utilization, high activity, and simple preparation methods
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[0032] 1. Preparation of antioxidant peptides:
[0033] 1-1. Extraction of chicken breast myofibrillar protein:
[0034] Remove visible adipose connective tissue from the chicken breast, chop it into minced meat with a knife, add four times the volume of protein extract to homogenize for 25 seconds, centrifuge at 5000r / min for 15 minutes at 4°C, discard the supernatant and save the precipitate. Add protein extract and repeat the above operation twice to obtain crude protein. The composition of protein extraction solution used is: the concentration of NaCl is 0.1mol / L, MgCl 2 The concentration of 0.002mol / L, the concentration of EDTA·2Na is 0.001mol / L, NaH 2 PO 4 / Na 2 HPO 4 The concentration is 0.1mol / L, and the pH value is 7.0.
[0035] The obtained crude protein was mixed with 4 times the volume concentration of 0.1 mol / L NaCl solution, homogenized for 25 s, centrifuged at 5000 r / min for 15 min at 4 °C, and the supernatant was discarded to save the precipitate. Then a...
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