Antioxidant peptide and preparation method and application thereof

A technology of antioxidant peptides and antioxidant activity, applied in the biological field, can solve problems such as few studies on functional characteristics, and achieve high-value utilization, high activity, and simple preparation methods

Active Publication Date: 2020-09-11
TIANJIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peptides with good antioxidant activity have been obtained from plant protein (such as soybean protein and oilseed protein), milk protein, and marine organisms, but studies based on antioxidant peptides derived from meat protein and their functional properties are relatively limited. few

Method used

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  • Antioxidant peptide and preparation method and application thereof
  • Antioxidant peptide and preparation method and application thereof
  • Antioxidant peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Preparation of antioxidant peptides:

[0033] 1-1. Extraction of chicken breast myofibrillar protein:

[0034] Remove visible adipose connective tissue from the chicken breast, chop it into minced meat with a knife, add four times the volume of protein extract to homogenize for 25 seconds, centrifuge at 5000r / min for 15 minutes at 4°C, discard the supernatant and save the precipitate. Add protein extract and repeat the above operation twice to obtain crude protein. The composition of protein extraction solution used is: the concentration of NaCl is 0.1mol / L, MgCl 2 The concentration of 0.002mol / L, the concentration of EDTA·2Na is 0.001mol / L, NaH 2 PO 4 / Na 2 HPO 4 The concentration is 0.1mol / L, and the pH value is 7.0.

[0035] The obtained crude protein was mixed with 4 times the volume concentration of 0.1 mol / L NaCl solution, homogenized for 25 s, centrifuged at 5000 r / min for 15 min at 4 °C, and the supernatant was discarded to save the precipitate. Then a...

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Abstract

The invention discloses an antioxidant peptide and a preparation method and application thereof, and aims to provide an antioxidant peptide which is derived from chicken breast myofibrillar protein and has high-efficiency antioxidant activity, and a preparation method and application thereof. The amino acid sequence of the antioxidant peptide is ITTNPYDY or IGWSPLGSL. The preparation method of theantioxidant peptide comprises the following steps of: extracting the myofibrillar protein from fresh chicken breast serving as a raw material; performing enzymolysis on the obtained myofibrillar protein by using pepsin; and performing separation, purification and synthesis to obtain the antioxidant peptide. The antioxidant peptide is a novel antioxidant peptide, and has high antioxidant activitythrough detection; under the measuring condition, the iron ion reducing capacity and the oxygen radical absorbing capacity of the antioxidant peptide are obviously higher than those of glutathione; and meanwhile, the antioxidant peptide has an obvious effect of inhibiting the oxidative damage of NIH-3T3 cells. The antioxidant peptide belongs to a natural antioxidant, is safer to eat, and can be applied to food, health-care products and medicaments with antioxidant functions.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to an antioxidant peptide derived from chicken breast myofibrillar protein and its preparation method and application. Background technique [0002] Excessive reactive oxygen species (ROS) free radicals will destroy the normal redox balance of the body, causing oxidative damage to intracellular biomacromolecules such as proteins, lipids, DNA, etc., thereby accelerating the aging of the body, causing neurodegenerative diseases and atherosclerosis , chronic inflammation, cancer and many other diseases. Studies have found that dietary intake of antioxidants to remove excessive free radicals in the body and reduce the level of oxidative stress in the body is an effective method, which can prevent and even treat certain diseases. At present, some synthetic antioxidants, such as butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), tert-butylhydroquinone (TBHQ), etc., are wid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K14/465C07K1/36C07K1/34C07K1/16C07K1/04C12P21/06A23L33/18A61P39/06
CPCC07K14/78C07K14/465C12P21/06A23L33/18A61P39/06A23V2002/00A23V2200/30
Inventor 陈金玉曲金萍张坤生闫怡君张蕾蕾郑嘉玉
Owner TIANJIN UNIV OF COMMERCE
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