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A kind of Bacillus paralicheniformis and the method for preparing bio-nano-selenium-enriched probiotics

A Bacillus licheniformis and bio-nano technology, which is applied in the field of microbial fermentation, can solve problems such as potential toxicity of products, and achieve the effects of improving antioxidant activity, repairing oxidative damage and improving economic benefits.

Active Publication Date: 2018-07-17
ZHEJIANG ESIGMA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the method disclosed by CN105420280A using Bacillus licheniformis to biosynthesize nano-selenium, the reduction rate of sodium selenite can only reach about 80%, and the product has potential toxicity

Method used

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  • A kind of Bacillus paralicheniformis and the method for preparing bio-nano-selenium-enriched probiotics
  • A kind of Bacillus paralicheniformis and the method for preparing bio-nano-selenium-enriched probiotics
  • A kind of Bacillus paralicheniformis and the method for preparing bio-nano-selenium-enriched probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1B

[0027] Example 1 takes B. paralicheniformis Preparation of bionano selenium-enriched probiotics with ZJUSE-1 and sodium selenite

[0028] 1. Culture medium preparation

[0029] LB plate medium: NaCl 10g, tryptone 10g, yeast extract 5g, agar 20g, deionized water 1L, high temperature sterilization at 121°C for 21min.

[0030] Seed medium: glucose 15g, tryptone 4g, yeast extract 6g, NaCl 10g, deionized water 1L, high temperature sterilization at 121°C for 21min.

[0031] Fermentation medium: glucose 20g, tryptone 10g, yeast extract 10g, K 2 HPO 4 1g, NaCl 5g, MgSO 4 15g, 1L deionized water, initial pH 7.0, high temperature sterilization at 115℃ for 30min.

[0032] 2. Strain activation

[0033] frozen at -80°C B. paralicheniformis Thaw the ZJUSE-1 strain at room temperature, take a ring and streak it on the LB plate medium, and incubate at 37°C for 12 hours.

[0034] Pick a single colony from the plate medium and inoculate it in the seed medium, and cultivate it at 37°...

Embodiment 2

[0041] Example 2 B. paralicheniformis Analysis of the Tolerance Ability of ZJUSE-1 to Sodium Selenite

[0042] 1. Medium configuration

[0043] Prepare LB plate medium containing sodium selenite at gradient concentrations (0, 5, 10, 15, 20mM).

[0044] 2. Strain activation

[0045] frozen at -80°C B. paralicheniformis ZJUSE-1 strains were thawed at room temperature, and one ring was streaked on each selenium-containing LB plate medium, and cultured at 37°C for 24 hours.

[0046] 3. Analysis of results

[0047] After the incubation, the results showed that B. paralicheniformis ZJUSE-1 can grow in LB plate medium containing up to 15mM sodium selenite.

[0048] Sodium selenite belongs to the 6.1 class of highly toxic chemicals. In the production of nano-selenium, if there is sodium selenite residue in the product, it is very easy to cause harm to subsequent production and application.

[0049] In the preparation method disclosed by CN1706459A using selenium-enriched la...

Embodiment 3

[0050] Example 3 Characteristic analysis of bio-nano-selenium-enriched probiotics

[0051] 1. Field SEM results

[0052] Get the bionano selenium-enriched probiotic product in Example 1 and fix it with 2.5% glutaraldehyde for 12 hours. Washed with PBS, fixed with 1% osmic acid, dehydrated with alcohol gradient, treated with isoamyl acetate, dried at the critical point, coated, and observed under a field emission scanning electron microscope.

[0053] The result is as figure 1 Shown: B. paralicheniformisZJUSE-1 reduces sodium selenite to generate bionano-selenium, and secretes organic matter to wrap and release bionano-selenium to the extracellular space. The arrow points to the biological nano-selenium.

[0054] 2. Fourier Transform Infrared Transform Spectroscopy Results

[0055] Take the biological nano-selenium-enriched probiotic product in Example 1 and dry it in an oven at 60° C. for 12 hours. Take the dried product and detect it by Fourier transform infrared tran...

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Abstract

The invention discloses a bacillus paralicheniformis and a method for preparing bio-nano-selenium-enriched probiotics. Bacillus paralicheniformis, named Bacillus paralicheniformis ZJUSE‑1, was preserved in the China General Microorganism Culture Collection and Management Center on March 21, 2017, with a preservation number: CGMCC No.13908. After fermentation, bio-nano selenium-rich probiotics were obtained. Bio-nanometer selenium is coated with secretions containing polysaccharides and protein characteristic groups, has antioxidant activity, and can scavenge DPPH free radicals and superoxide anions. Bio-nanometer selenium and probiotics play a synergistic effect, which can effectively repair the oxidative damage of the body, relieve the oxidative stress caused by weaning of the young, and improve the economic benefits of breeding production.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to the preparation of bio-enriched nano selenium probiotics and their anti-oxidation application. Background technique [0002] Selenium is an essential trace element for animals and humans. It participates in multiple major metabolic pathways in the body and is essential to health. Studies have shown that selenium has multiple functions such as anti-cancer and anti-oxidation, improving immunity, preventing and treating Keshan disease, Kaschin-Beck disease, and regulating body metabolism. [0003] In recent years, nano-selenium, as a new form of selenium addition with high biological activity and high safety, has attracted widespread attention. However, the synthesis of nano-selenium is often prepared by chemical methods, that is, using reducing agents such as ascorbic acid and sodium thiosulfate to reduce selenate or selenite under the condition of additionally addi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P9/00A61P39/06C12R1/07
CPCC12N1/20C12P9/00C12N1/205C12R2001/07
Inventor 汪以真程远之肖肖宋德广李笑笑路则庆王凤芹
Owner ZHEJIANG ESIGMA BIOTECH CO LTD
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