Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus paracasei capable of relieving gastrointestinal injury caused by capsaicin and application of lactobacillus paracasei

A Lactobacillus and paracheese technology, applied in the field of microorganisms, can solve the problems of unclear relationship between gastrointestinal injury and lack of anti-spicy, and achieve the effects of improving gastric mucosal cell vacuolization, wide application prospects, and reducing serum neuropeptide levels.

Active Publication Date: 2021-07-16
JIANGNAN UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, it has been found that the probiotics that relieve gastrointestinal damage only interact with the capsaicin receptor TRPV1, and the relationship with capsaicin-induced gastrointestinal damage is unclear
Therefore, there is also a lack of probiotic preparations or fermented products on the market that relieve spicy heat and protect the stomach. It is urgent to find a probiotic that can relieve gastrointestinal damage caused by capsaicin, so as to expand the application of probiotics in daily diet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus paracasei capable of relieving gastrointestinal injury caused by capsaicin and application of lactobacillus paracasei
  • Lactobacillus paracasei capable of relieving gastrointestinal injury caused by capsaicin and application of lactobacillus paracasei
  • Lactobacillus paracasei capable of relieving gastrointestinal injury caused by capsaicin and application of lactobacillus paracasei

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0051] The preparation method of the lactobacillus suspension involved in the following examples is as follows:

[0052] Streak the Lactobacillus on the MRS solid medium and culture it at 37°C for 48 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, culture it at 37°C for 18 hours for activation, and activate two generations continuously to obtain Activation solution; the activation solution was inoculated into MRS liquid medium at an inoculum size of 2% (v / v), and cultured at 37°C for 18 hours to obtain a bacterial liquid; the bacterial liquid was centrifuged at 4°C and 6000×g After 10 minutes, the Lactobacillus cells were obtained; the Lactobacillus cells were washed 3 times with normal saline, and then resuspended in a sucrose solution with a concentration of 300g / L to a bacterial concentration of 1×10 9 CFU / mL to obtain a bacterial suspension, and store the bacterial suspension at 80°C until use.

Embodiment 1

[0053] Embodiment 1: Screening and identification of Lactobacillus paracasei CCFM1176

[0054] 1. Screening

[0055] Taking dairy products from Hulunbeier City, Inner Mongolia Province as samples, the samples were pretreated and stored in 30% glycerin in a -80°C refrigerator. After taking out and thawing, mix the samples and add 0.5mL samples to 4.5mL 0.9% physiological Perform gradient dilution with saline, select the appropriate gradient dilution and spread it on the MRS solid medium, incubate at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS plate, pick a single colony and transfer it to the liquid MRS liquid medium Bacteria enrichment, 30% glycerol preservation, to obtain strain CCFM1176.

[0056] 2. Identification

[0057] The whole genome DNA of bacterial strain CCFM1176 was extracted for the amplification of 16S rDNA, and the amplified DNA fragments were collected and sequenced (completed by Suzhou Jinweizhi Biotechnology Co., Ltd.). After se...

Embodiment 2

[0058] Embodiment 2: the cultivation of Lactobacillus paracasei CCFM1176

[0059] After inserting Lactobacillus paracasei CCFM1176 into the MRS solid medium and culturing at 37°C for 48 hours, the colonies were observed and observed under a microscope. It was found that the colonies were milky white semicircular protrusions with smooth and moist surfaces. Neat edges.

[0060] Lactobacillus paracasei CCFM1176 was inserted into MRS liquid medium and cultured at 37°C for 48 hours. During the cultivation process, the pH of the culture solution was measured by a pH meter at intervals, and it was found that Lactobacillus casei CCFM1176 produced acid during the cultivation process.

[0061] Insert Lactobacillus paracasei CCFM1176 into MRS liquid medium and culture them at 10-50°C for 48 hours. During the cultivation process, measure the OD of the culture solution with a microplate reader at intervals 600 It was found that Lactobacillus casei CCFM1176 grew best at 30-37°C, and reache...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses lactobacillus paracasei capable of relieving gastrointestinal injury caused by capsaicin and an application of the lactobacillus paracasei, and belongs to the technical field of microorganisms. The lactobacillus paracasei CCFM1176 can relieve oxidative stress of the mouse liver caused by capsaicin and reduce the level of serum neuropeptide of the mouse liver. The damages such as gastric mucosa cell vacuolation, intestinal villus shedding, colon goblet cell reduction and inflammatory cell infiltration caused by capsaicin can be improved; and the transcriptional level of mouse gastrointestinal tissue inflammation marker protein can be reduced. The lactobacillus paracasei CCFM1176 is used for preparing a pharmaceutical composition and fermented food for relieving pungency and protecting intestines and stomach, and has a very wide application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus paracasei capable of alleviating gastrointestinal damage caused by capsaicin and its application, belonging to the technical field of microbes. Background technique [0002] Chili pepper is a kind of flavor spice, which is widely processed and eaten because of its unique spicy taste. The main source of pungency in pepper fruit is capsaicinoids. The molecular formula of capsaicin is C 18 h 27 NO 3 , whose chemical formula is trans-8-methyl-N-vanillyl-6-nonenamide, is an extremely pungent vanillyl amide alkaloid. [0003] Due to the strong irritant of capsaicin, adverse sensations such as burning, heartburn, and pain appear in the digestive tract after ingestion. At the same time, it has a certain negative impact on digestion and metabolism, gastrointestinal mucosa, intestinal flora, and specific functional gastrointestinal diseases. The specific manifestations are as follows: the level of oxidative...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23C9/123A23C13/16A23C19/032A23C19/06A23L11/65A23L11/50A23L19/00A23L33/135A61K35/747A61P1/00C12R1/225
CPCC12N1/20A23C9/1234A23C9/1238A23C13/16A23C19/0323A23C19/062A23C11/106A23L19/00A23L33/135A61K35/747A61P1/00A23V2002/00A23V2400/165A23V2400/123A23V2400/113A23V2400/249A23V2200/30A23V2200/32
Inventor 毛丙永项群然崔树茂唐鑫陆文伟刘小鸣赵建新陈卫
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com