Application of lactobacillus rhamnosus LRa05 in preparation of preparation for improving irritable bowel syndrome
A technology of Lactobacillus rhamnosus and irritable bowel syndrome, applied in the application field of preparations, can solve the problems of limited ability to relieve irritable bowel syndrome and the inability of prebiotic ingredients to promote Lactobacillus rhamnosus, etc. Achieve the effect of increasing the level of anti-inflammatory factors, no toxic side effects, and high practicability
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preparation example
[0062] This preparation example provides a freeze-dried powder containing Lactobacillus rhamnosus LRa05, and the freeze-dried powder containing Lactobacillus rhamnosus LRa05 is prepared by the following preparation method:
[0063] (a) Lactobacillus rhamnosus LRa05 stored in glycerol tube was inoculated into MRS liquid medium at a proportion of 2%, cultured at 37°C for 18 hours, and activated for 2 generations; the activated seeds were inoculated at 2% Into the fermenter, incubate at 37°C for 12h, pH control to 5.5, centrifuge the bacterial liquid at 7000r / min for 12min, discard the supernatant, collect the bacterial slurry,
[0064] (b) adding a mixed protective agent containing trehalose, maltodextrin, and sucrose to the obtained bacterial slurry, and the protective agent contains 25% trehalose, 10% maltodextrin, 2% sucrose, and 63% pure water. The mass ratio of the agent to the bacterial slurry is 1:1, and then the freeze-dried powder is prepared after vacuum freeze-drying....
Embodiment 1
[0066] To study the improvement effect of Lactobacillus rhamnosus LRa05 lyophilized powder on IBS
[0067] (1) Construction of animal models:
[0068] Select 72 6-8 week old SD rats, female, weighing 200-220 g, adaptively reared for 1 week after purchase, with a light-dark cycle of 12 h, room temperature controlled at 20-25 ℃, free feeding and water, rat diet containing protein , corn oil, carbohydrates, etc. Seventy-two SD rats were evenly divided into normal group, IBS group and LRa05 group according to their body weight status, with 24 rats in each group, and the IBS group and LRa05 group were used to construct animal models by chemical stimulation. The specific method is as follows: dilute the purchased mustard oil with corn oil to a concentration of 5%, suck 0.5 mL with a 5cm long trocar, and perform an enema every morning after the rat defecates, and inject it when the length of the rat rectum is 5cm, once a day , continuous enema for 2 weeks, at the same time, LRa05 g...
Embodiment 2-6
[0084] Embodiment 2-6 provides the solid beverage containing Lactobacillus rhamnosus LRa05 of five different content formulas, and the formula of described solid beverage is specifically as shown in Table 2 below:
[0085] Table 2
[0086]
[0087] The preparation method of the solid beverage comprises the following steps:
[0088] The freeze-dried powder containing Lactobacillus rhamnosus LRa05, fructooligosaccharides, xylo-oligosaccharides, galactooligosaccharides, isomaltose and inulin were mixed at 25 ℃ for 30 min at the speed of 20 r / min according to the above ratio. After mixing, the solid beverage containing Lactobacillus rhamnosus LRa05 is obtained.
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