Application of lactobacillus rhamnosus LRa05 in preparation of preparation for improving irritable bowel syndrome

A technology of Lactobacillus rhamnosus and irritable bowel syndrome, applied in the application field of preparations, can solve the problems of limited ability to relieve irritable bowel syndrome and the inability of prebiotic ingredients to promote Lactobacillus rhamnosus, etc. Achieve the effect of increasing the level of anti-inflammatory factors, no toxic side effects, and high practicability

Pending Publication Date: 2022-07-22
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN112654358A discloses a combination of Lactobacillus for alleviating irritable bowel syndrome and for alleviating other gastrointestinal diseases. The composition and method involve live Lactobacillus bacteria, especially live Lactobacillus acidophilus and live Lactobacillus casei Use of a combination with live Lactobacillus rhamnosus for alleviating undesirable gastrointestinal health problems such as irritable bowel syndrome (IBS), abdominal pain, abdominal discomfort, bloating, liquid stools and Constipation, but the Lactobacillus rhamnosus with the deposit number CNCM I-3990 disclosed in this application has limited ability to relieve irritable bowel syndrome, so it needs to meet the requirements of live Lactobacillus acidophilus and live Lactobacillus casei to relieve irritable bowel syndrome Syndrome and for the purpose of alleviating other gastrointestinal disorders
The prebiotic ingredients in the freeze-dried powder formula cannot achieve the purpose of promoting Lactobacillus rhamnosus LRa05 in the intestinal tract

Method used

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  • Application of lactobacillus rhamnosus LRa05 in preparation of preparation for improving irritable bowel syndrome
  • Application of lactobacillus rhamnosus LRa05 in preparation of preparation for improving irritable bowel syndrome
  • Application of lactobacillus rhamnosus LRa05 in preparation of preparation for improving irritable bowel syndrome

Examples

Experimental program
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preparation example

[0062] This preparation example provides a freeze-dried powder containing Lactobacillus rhamnosus LRa05, and the freeze-dried powder containing Lactobacillus rhamnosus LRa05 is prepared by the following preparation method:

[0063] (a) Lactobacillus rhamnosus LRa05 stored in glycerol tube was inoculated into MRS liquid medium at a proportion of 2%, cultured at 37°C for 18 hours, and activated for 2 generations; the activated seeds were inoculated at 2% Into the fermenter, incubate at 37°C for 12h, pH control to 5.5, centrifuge the bacterial liquid at 7000r / min for 12min, discard the supernatant, collect the bacterial slurry,

[0064] (b) adding a mixed protective agent containing trehalose, maltodextrin, and sucrose to the obtained bacterial slurry, and the protective agent contains 25% trehalose, 10% maltodextrin, 2% sucrose, and 63% pure water. The mass ratio of the agent to the bacterial slurry is 1:1, and then the freeze-dried powder is prepared after vacuum freeze-drying....

Embodiment 1

[0066] To study the improvement effect of Lactobacillus rhamnosus LRa05 lyophilized powder on IBS

[0067] (1) Construction of animal models:

[0068] Select 72 6-8 week old SD rats, female, weighing 200-220 g, adaptively reared for 1 week after purchase, with a light-dark cycle of 12 h, room temperature controlled at 20-25 ℃, free feeding and water, rat diet containing protein , corn oil, carbohydrates, etc. Seventy-two SD rats were evenly divided into normal group, IBS group and LRa05 group according to their body weight status, with 24 rats in each group, and the IBS group and LRa05 group were used to construct animal models by chemical stimulation. The specific method is as follows: dilute the purchased mustard oil with corn oil to a concentration of 5%, suck 0.5 mL with a 5cm long trocar, and perform an enema every morning after the rat defecates, and inject it when the length of the rat rectum is 5cm, once a day , continuous enema for 2 weeks, at the same time, LRa05 g...

Embodiment 2-6

[0084] Embodiment 2-6 provides the solid beverage containing Lactobacillus rhamnosus LRa05 of five different content formulas, and the formula of described solid beverage is specifically as shown in Table 2 below:

[0085] Table 2

[0086]

[0087] The preparation method of the solid beverage comprises the following steps:

[0088] The freeze-dried powder containing Lactobacillus rhamnosus LRa05, fructooligosaccharides, xylo-oligosaccharides, galactooligosaccharides, isomaltose and inulin were mixed at 25 ℃ for 30 min at the speed of 20 r / min according to the above ratio. After mixing, the solid beverage containing Lactobacillus rhamnosus LRa05 is obtained.

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Abstract

The invention provides an application of lactobacillus rhamnosus LRa05 in preparation of a preparation for improving irritable bowel syndrome. The lactobacillus rhamnosus LRa05 freeze-dried powder provided by the invention has a remarkable curative effect on the irritable bowel syndrome, and can be used for reducing serum endotoxin, reducing proinflammatory factors, increasing the level of anti-inflammatory factors, relieving oxidative stress and adjusting intestinal flora disorder, so that the intestinal flora is healthy. The lactobacillus rhamnosum LRa05 freeze-dried powder product provided by the invention is simple to prepare and suitable for large-scale production, has the characteristics of simple treatment, high remission rate, no toxic or side effect and the like as an auxiliary medicine of IBS, and is relatively high in practicability.

Description

technical field [0001] The invention belongs to the field of nutrition and health food, and particularly relates to the application of Lactobacillus rhamnosus LRa05 in the preparation of a preparation for improving irritable bowel syndrome. Background technique [0002] Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders. Epidemiological studies around the world have shown that IBS is a frequently-occurring disease worldwide. The prevalence of population in Western countries is 10%-20%. An epidemiological survey in my country according to Rome Ⅱ criteria showed that the prevalence of IBS in community population was 5.7%, of which 22% had visited a doctor because of IBS symptoms. IBS is a disease characterized by abdominal pain, bloating, or abdominal discomfort as the main symptom, which is associated with defecation or accompanied by changes in defecation habits such as frequency and / or stool quality. Although IBS is not life-thre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L2/39A23L33/21A61K35/747A61P1/00A61P1/12
CPCA23L33/135A23L2/39A23L33/21A61K35/747A61P1/00A61P1/12A23V2002/00A23V2400/175A23V2200/3202A23V2200/3204A23V2250/284A23V2250/5062A23V2250/636A23V2250/5114A23V2250/628
Inventor 方曙光武岩峰郭晓娟汪欣韩梅朱建国
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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