Ceruloplasmin detection kit and preparation method thereof

Abstract

The invention discloses a ceruloplasmin detection kit which comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a PBS buffer solution, magnesiumchloride, trehalose, triton-100, hydroxylamine hydrochloride, sodium azide and PEG-8000; and the reagent R2 comprises the following components: a PBS buffer solution, a latex-coated copper cyanin antibody solution and bovine serum albumin. The preparation method comprises the following steps: (A) the preparation of the reagent R1: adding purified water into a blending tank A, sequentially adding the components of the reagent R1 into a blending tank, mixing and stirring, adjusting the pH value of the solution, and filtering by using a microporous filter membrane to obtain the reagent R1; and (B) the preparation of the reagent R2: adding purified water into a blending tank B, sequentially adding the components of the reagent R2 into a blending tank B, mixing and stirring, adjusting the pH value of the solution, and filtering by using a microfiltration membrane to obtain the reagent R2. By adopting the scheme disclosed by the invention, the detection efficiency is improved, and the clinical disease detection cost is reduced.

Description

Technical field [0001] The invention relates to a preparation method of a ceruloplasmin detection kit, in particular to a ceruloplasmin detection kit and a preparation method thereof. Background technique [0002] In urine testing, ceruloplasmin content testing is a routine item in urine testing, and some clinical diseases can be judged by detecting the ceruloplasmin content in urine. [0003] Specifically, existing clinical studies have shown that the content of ceruloplasmin in urine is affected by human liver function, kidney function, and biliary function. Specifically, the liver can synthesize ceruloplasmin, while the biliary tract and kidneys decompose and excrete. The main method of ceruloplasmin, therefore, detecting the content of ceruloplasmin in urine has important clinical diagnostic significance for the prevention and diagnosis of some clinical diseases. [0004] However, the main method of clinically detecting the binding capacity of ceruloplasmin still uses the more t...

AI Technical Summary

Problems solved by technology

[0004] However, the main way to detect the binding capacity of ceruloplasmin clinically is still using the more traditional enzyme-linked immunosorbent assay.

[0005] The above detection methods are not only backward, but also have a long detection cycle and high detection cost, which not only increases the cost of clinical detection but also reduces the detection efficiency

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