Composition of transiron protein and biological reducing agent and its preparation method

A transferrin and conjugate technology, applied in the directions of drug combination, peptide/protein components, nitro compound active ingredients, etc., can solve problems such as poor targeting and allergic reactions, and achieve the effect of improving curative effect and reducing tolerance

Inactive Publication Date: 2005-10-26
NANJING UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example: Antibodies usually bind to normal tissues and can even cause severe allergic reactions, but microsomes are poorly targeted

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 51.3 mg of glutaric anhydride was added to 10 ml of tetrahydrofolic acid containing 50 mg, and the mixture was heated to 60° C. under nitrogen protection for 10 hours. Distilled after the reaction was complete. The residue was dissolved in 2 ml of methanol, and methanol was used as the mobile phase, and passed through a Sephadex L-20 (2.5×97 cm) chromatographic column for column chromatography. The fractions were evaporated to dryness to obtain G-MMC (glutaraldehyde esterified mitomycin). Dissolve 56.0 mg of G-MMC in 3.4 ml of acetonitrile, add 21.6 mg of N-hydroxysuccinimide (HOSu) and 155.6 mg of DDC, and the mixture is 2 Stir at 4°C for 48 hours under protected conditions. Then 7ml of ice water was added, filtered, the filtrate was extracted three times with 10ml of chloroform, and the chloroform was evaporated to dryness to obtain MMC-G-OSu, which was recrystallized from n-hexyl butyrate to obtain a purple powder.

[0019] Dissolve 30 mg of transferrin in 3 ml of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A compound of the active anticancer protein and bioreducer for preparing the medicine to treat cancer is prepared from the target protein chosen from transferrin, ceruloplasmin, somatostatin, vitamine bindin, etc and the bioreducer chosen from bifunctional heterocyclic nitro compound, quinone, heterocyclic NOx, topoisomerase B inhibitor and DNA-targeting medicine.

Description

1. Technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a combination of a protein and a biological reducing agent effective in treating cancer, especially a combination of a targeting protein and a biological reducing agent. 2. Background technology [0002] At present, the two biggest problems encountered in the treatment of tumors are drug resistance and drug toxicity. One way to solve drug resistance and drug toxicity is to target drugs to tumor cells. Many researchers have devoted themselves to the study of using antibodies and other means to achieve the goal of targeting. Although these have achieved some results, they have also attracted a lot of attention. series of new questions. For example, antibodies usually bind to normal tissues and can even cause severe allergic reactions, while microsomes are poorly targeted. [0003] Transferrin (Transferrin) is a β1 globulin with a molecular weight of abou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K38/31A61K38/40A61K47/42A61K47/48
CPCA61K38/40A61K47/483A61K38/1808A61K47/48246A61K38/31A61K31/04A61K38/1709A61K31/28A61K47/48107A61K31/122A61K47/551A61K47/64A61K47/644A61P35/00
Inventor 胡一桥吴锦慧罗玲英支枫
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap