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Ceruloplasmin detection kit and use method thereof

A detection kit and ceruloplasmin technology, applied in the field of medical testing, can solve the problems of poor quantitative detection effect and cumbersome operation of enzyme-linked immunosorbent assay, and achieve simple operation, high detection sensitivity, accuracy and stability good effect

Inactive Publication Date: 2018-02-27
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method for detecting ceruloplasmin is mainly the enzyme-linked immunosorbent assay, but the specific operation of the enzyme-linked immunosorbent assay is relatively cumbersome, and the quantitative detection effect is not good, and the results are greatly affected by human factors

Method used

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  • Ceruloplasmin detection kit and use method thereof
  • Ceruloplasmin detection kit and use method thereof
  • Ceruloplasmin detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A ceruloplasmin detection kit according to this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:

[0051] Reagent R1:

[0052]

[0053] The solvent is purified water;

[0054] Reagent R2:

[0055]

[0056] The solvent is purified water;

[0057] The raw material components of the latex-coated ceruloplasmin antibody are: latex microspheres, ceruloplasmin antibody and polyethylene-p-chloromethylstyrene copolymer, wherein the polyethylene-p-chloromethylstyrene copolymer is in the overall reagent The content in R2 is 9%.

Embodiment 2

[0059] A ceruloplasmin detection kit according to this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:

[0060] Reagent R1:

[0061]

[0062] The solvent is purified water;

[0063] Reagent R2:

[0064]

[0065] The solvent is purified water;

[0066] The raw material components of the latex-coated ceruloplasmin antibody are: latex microspheres, ceruloplasmin antibody and polyethylene-p-chloromethylstyrene copolymer, wherein the polyethylene-p-chloromethylstyrene copolymer is in the overall reagent The content in R2 was 11%.

Embodiment 3

[0068] A ceruloplasmin detection kit according to this embodiment includes two liquid components of reagent R1 and reagent R2 that are independent of each other, and the components and corresponding contents included are:

[0069] Reagent R1:

[0070]

[0071]

[0072] The solvent is purified water;

[0073] Reagent R2:

[0074]

[0075] The solvent is purified water;

[0076] The raw material components of the latex-coated ceruloplasmin antibody are: latex microspheres, ceruloplasmin antibody and polyethylene-p-chloromethylstyrene copolymer, wherein the polyethylene-p-chloromethylstyrene copolymer is in the overall reagent The content in R2 is 10%.

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Abstract

The invention discloses a ceruloplasmin detection kit which comprises a reagent R1 containing a PBS buffer solution, NaCl, NaN3, trehalose, triton and PEG-8000, with a solvent being purified water, areagent R2 containing a PBS buffer solution, NaCl, NaN3, BSA, Arabic gum and a latex-coated ceruloplasmin antibody, with a solvent being purified water, wherein the raw materials of the latex-coated ceruloplasmin antibody include latex microspheres, a ceruloplasmin antibody and a polyethylene p-chloromethyl styrene copolymer. The invention also discloses a use method of the ceruloplasmin detectionkit. The ceruloplasmin detection kit and the use method of the ceruloplasmin detection kit have the following advantages: (1) the kit provided by the invention has higher detection sensitivity and accuracy, and is simple and fast to use and operate, and detection can be completed within only 6 minutes; (2) an antigen-antibody complex formed according to the invention has high stability, certainabsorbance at a specific wavelength and strong specificity; and (3) the kit is used for a full-automatic biochemical analyzer, and can be developed and promoted on a large scale.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a ceruloplasmin detection kit and a use method thereof. Background technique [0002] Ceruloplasmin (CER), also known as copper oxidase, is a copper-containing α2 glycoprotein with a molecular weight of about 120,000-160,000 and is not easy to purify. As far known, ceruloplasmin is a single-chain polypeptide with 6-7 copper atoms per molecule. It is blue due to the copper content and contains about 10% sugar. The terminal sialic acid is connected to the polypeptide chain and has a genetic Polymorphism. Its role is to regulate the distribution of copper in various parts of the body and to synthesize copper-containing enzyme proteins. It has the function of antioxidant and oxidase activity, and has the ability to catalyze the oxidation of polyphenols and polyamines. It is generally believed that ceruloplasmin is synthesized by the liver, a part is excreted by the biliary ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 吴铮朱雨丁先骏庄庆华吴泽东朱卫中
Owner ANHUI IPROCOM BIOTECH CO LTD
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