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Preparation method of ceruloplasmin detection kit

A detection kit, ceruloplasmin technology, applied in the field of medical testing, can solve the problems of tedious enzyme-linked immunosorbent assay, poor quantitative detection effect, etc., and achieves improved sensitivity and linear range, simple operation and good stability. Effect

Inactive Publication Date: 2018-02-27
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method for detecting ceruloplasmin is mainly the enzyme-linked immunosorbent assay, but the specific operation of the enzyme-linked immunosorbent assay is relatively cumbersome, and the quantitative detection effect is not good, and the results are greatly affected by human factors

Method used

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  • Preparation method of ceruloplasmin detection kit
  • Preparation method of ceruloplasmin detection kit
  • Preparation method of ceruloplasmin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method of a ceruloplasmin detection kit of the present embodiment comprises the following steps:

[0038] (1) Prepare reagent R1 according to the following component contents:

[0039] Reagent R1:

[0040]

[0041] The solvent is purified water;

[0042] ① According to the component content of the above reagent R1, prepare PBS buffer solution, adjust the pH, and use it as R1 buffer solution;

[0043] ②According to the component content of the above reagent R1, NaCl, NaN 3 , trehalose, triton, and PEG-8000 were dissolved in the R1 buffer, stirred evenly, and the reagent R1 was prepared after all the raw materials were fully dissolved;

[0044] (2) Prepare reagent R2 according to the following component contents:

[0045] Reagent R2:

[0046]

[0047] The solvent is purified water;

[0048] ① According to the component content of the above reagent R2, prepare PBS buffer solution, adjust the pH, and use it as R2 buffer solution;

[0049] ②Accordi...

Embodiment 2

[0056] A preparation method of a ceruloplasmin detection kit of the present embodiment comprises the following steps:

[0057] (1) Prepare reagent R1 according to the following component contents:

[0058] Reagent R1:

[0059]

[0060]

[0061] The solvent is purified water;

[0062] ① According to the component content of the above reagent R1, prepare PBS buffer solution, adjust the pH, and use it as R1 buffer solution;

[0063] ②According to the component content of the above reagent R1, NaCl, NaN 3 , trehalose, triton, and PEG-8000 were dissolved in the R1 buffer, stirred evenly, and the reagent R1 was prepared after all the raw materials were fully dissolved;

[0064] (2) Prepare reagent R2 according to the following component contents:

[0065] Reagent R2:

[0066]

[0067] The solvent is purified water;

[0068] ① According to the component content of the above reagent R2, prepare PBS buffer solution, adjust the pH, and use it as R2 buffer solution;

[0069...

Embodiment 3

[0076] A preparation method of a ceruloplasmin detection kit of the present embodiment comprises the following steps:

[0077] (1) Prepare reagent R1 according to the following component contents:

[0078] Reagent R1:

[0079]

[0080] The solvent is purified water;

[0081] ① According to the component content of the above reagent R1, prepare PBS buffer solution, adjust the pH, and use it as R1 buffer solution;

[0082] ②According to the component content of the above reagent R1, NaCl, NaN 3 , trehalose, triton, and PEG-8000 were dissolved in the R1 buffer, stirred evenly, and the reagent R1 was prepared after all the raw materials were fully dissolved;

[0083] (2) Prepare reagent R2 according to the following component contents:

[0084] Reagent R2:

[0085]

[0086] The solvent is purified water;

[0087] ① According to the component content of the above reagent R2, prepare PBS buffer solution, adjust the pH, and use it as R2 buffer solution;

[0088] ②Accordi...

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Abstract

The invention discloses a preparation method of a ceruloplasmin detection kit, which comprises the following steps: (1) preparing a PBS buffer solution according to component contents of a reagent R1,adjusting the pH value to obtain an R1 buffer solution, and dissolving NaCl, NaN3, trehalose, triton and PEG-8000 in the R1 buffer solution to obtain the reagent R1; and (2) preparing a PBS buffer solution according to component contents of a reagent R2, adjusting the pH value to obtain an R2 buffer solution, dissolving NaCl, NaN3, BSA and Arabic gum in the R2 buffer solution to obtain an R2 dispersion liquid, preparing a latex-coated ceruloplasmin antibody, and dissolving the latex coated ceruloplasmin antibody by the R2 dispersion liquid to obtain a reagent R2. The preparation method provided by the invention has the following advantages: (1) the operation is simple and rapid; (2) the kit has high sensitivity and accuracy; (3) latex microspheres with different particle sizes are adoptedto improve the sensitivity and the linear range of the kit; (4) the kit has good stability and strong specificity; and (5) the kit is suitable for fully-automatic testing.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a preparation method of a ceruloplasmin detection kit. Background technique [0002] Ceruloplasmin (CER), also known as copper oxidase, is a copper-containing α2 glycoprotein with a molecular weight of about 120,000-160,000 and is not easy to purify. As far known, ceruloplasmin is a single-chain polypeptide with 6-7 copper atoms per molecule. It is blue due to the copper content and contains about 10% sugar. The terminal sialic acid is connected to the polypeptide chain and has a genetic Polymorphism. Its role is to regulate the distribution of copper in various parts of the body and to synthesize copper-containing enzyme proteins. It has the function of antioxidant and oxidase activity, and has the ability to catalyze the oxidation of polyphenols and polyamines. It is generally believed that ceruloplasmin is synthesized by the liver, a part is excreted by the biliary t...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54313
Inventor 吴铮朱雨丁先骏庄庆华吴泽东朱卫中
Owner ANHUI IPROCOM BIOTECH CO LTD
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