Disclosed is a method for perparation of biological structure HAP / beta-TCP tissue engineering bones, comprising 1.obtaining HAP by using spongy bones of cattle femoral bone lower part as raw material, treating same by removing bone marrow, lipid, and prorein, then rinsing, drying, and high-temperature processing, reacting HAP with NH4H2PO4, high-temperature processing again, and obtaining a biological structure HAP / beta-TCP material support frame; 2.isolating bone marrow stroma stem cells in vitro and seeding same in culture bottles, adding DMEM culture medium, cuturing at 37 DEG C with 5% CO2, changing the culture medium every 2-3 days, digesting cells with parenzyme as cells grow up to 90%, subculturing same, reserving same when the number of bone marrow stroma reaches to 106-107 / ml; 3. rinsing biolofical structure HAP / beta-TCP material support frame with ultrasonic, dropping the subcultured cells on the support frame homogeneously, induced culturing same, obtaining said biological structure HAP / beta-TCP tissue engineering bone when cells fill internal void of support material. The microstructure of disclosed biological structure HAP / beta-TCP tissue engineering bone is completely analogical with spongy bone, facilitates degradation of osteogenesis and support fame materials in vivo which can be controlled, and so accelerating bone recovery.