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Stem cells and devices for bone regeneration

A bone regeneration and stem cell technology, applied in the field of scaffolds, bone regeneration, and 3D printing scaffolds, can solve the problem that the processing resolution cannot meet the requirements of micro-scale feature manufacturing

Active Publication Date: 2020-08-07
ALFRED E MANN INST FOR BIOMEDICAL ENG AT THE UNIV OF SOUTHERN CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the processing resolution of macroscale MIP-SL cannot meet the requirements of microscale feature fabrication.

Method used

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  • Stem cells and devices for bone regeneration
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  • Stem cells and devices for bone regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0288] Embodiment 1. mouse CNCC and BMMSC culture

[0289] Four-week-old mice were euthanized according to USC IACUC-approved procedures and their mandibles were isolated, minced and digested with 2 mg / ml type I collagenase and 4 mg / ml dispase II in PBS for 1 hour at 37°C. The long bone marrow was flushed from the femur and tibia, centrifuged quickly and resuspended. Single-cell suspensions were obtained by passing cells through a 70 μm filter and inoculated in a medium supplemented with 20% FBS, 2 mM L-glutamine, 55 μM 2-mercaptoethanol, 100 U / ml penicillin, and 100 μg / ml streptavidin. Prime α-MEM in a 10 cm plate dish. The medium was changed after the initial incubation for 48 hours.

Embodiment 2

[0290] Example 2. Subcutaneous cell implantation

[0291] CNCC and BMMSC were mixed in ratios of 9:1, 5:5 and 1:9. Then add a total of 2×10 6 cells implanted under the skin of immunocompromised mice. Mice were euthanized and cells were harvested three months after implantation, fixed in 4% PFA, decalcified and sectioned for histological examination.

Embodiment 3

[0292] Example 3. Scaffold degradation and cytotoxicity

[0293] Polycaprolactone (PCL)-based 3D-printed scaffolds were implanted subcutaneously in immunocompromised mice and collected at various time points to test their degradation profile by measuring their dry weight.

[0294] To test the potential cytotoxicity of PCL scaffolds, NIH3T3 cells were incubated at 1 × 10 4 Cells / well were seeded in 96-well plates. After 24 hours of incubation to allow cell attachment, the PCL scaffolds were co-cultured in DMEM medium with 10% FBS for 72 hours. MTT cell assay to test cell viability was performed following the manufacturer's instructions (Abeam).

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Abstract

This invention relates to a bone regeneration product comprising at least one stem cell, at least one scaffold, and at least one stem cell. The stem cells suitable for this invention may comprise stemcells suitable for a dense bone regeneration, stem cells suitable for a spongy bone regeneration, or a combination thereof. The bone regeneration product may further comprise a growth factor. This invention also relates to a bone regeneration method and treatment of any bone that has a critical size defect. This invention also relates to a scaffold. This invention further relates to a 3D printedscaffold comprising hydroxyapatite (HA) and tricalcium phosphate (TCP). This invention also relates to a scaffold comprising a polymer. The polymer of this invention may be prepared by using photocurable polymers and / or monomers. The scaffold of this invention may comprise a growth factor and a small molecule. The small molecule may be a Smurf1 inhibitor.

Description

[0001] Cross References to Related Applications [0002] This application claims U.S. Provisional Patent Application 62 / 584,052, entitled "Stem Cells and Devices For Bone Regeneration," Attorney Docket No. 064693-0420, filed November 9, 2017 rights and interests, the entire content of said provisional patent application is incorporated herein by reference. technical field [0003] The present invention relates to a bone regeneration product comprising at least one stem cell and at least one scaffold. The present invention also relates to a bone regeneration product comprising at least one stem cell and at least one scaffold; wherein said scaffold comprises any scaffold suitable for carrying said at least one stem cell. The present invention also relates to a bone regeneration product comprising at least one stem cell and at least one scaffold; wherein said scaffold comprises any 3D printed scaffold suitable for carrying said at least one stem cell. The present invention rela...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06
CPCA61L27/12A61L27/18A61L27/3834A61L27/3847A61L27/46A61L27/54A61F2/28A61L2300/414A61L2430/02A61F2002/2817A61F2002/285A61F2002/2835C08L67/04A61L27/56A61L2300/204
Inventor 柴洋陈勇袁渊郭玉兴李向佳佐耶·约翰逊
Owner ALFRED E MANN INST FOR BIOMEDICAL ENG AT THE UNIV OF SOUTHERN CALIFORNIA
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