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Penicillium, as well as preparation method and application

A Penicillium, applied technology, applied in the direction of biochemical equipment and methods, microorganism-based methods, fungi, etc.

Inactive Publication Date: 2009-05-20
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on leaching vanadium in stone coal by biometallurgy technology at home and abroad

Method used

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  • Penicillium, as well as preparation method and application
  • Penicillium, as well as preparation method and application
  • Penicillium, as well as preparation method and application

Examples

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Embodiment 1

[0034] A Penicillium fungus that decomposes insoluble phosphorus and metal ores, and isolates and screens leaching strains of phosphorus, nickel, cobalt, vanadium, etc. Specific steps are as follows:

[0035] a. Grind the sample collected from the vanadium mine in Guangxi, China, put about 5 grams of the sample in a sterile petri dish, pour it into a solid inorganic salt basic medium at 33 or 34 or 35 or 36 or 37 ° C, and put it at 30 ° C After culturing for 7 days, single colonies were isolated. The composition of this solid inorganic salt basal medium is (according to literature agricultural system science and comprehensive research, 2003,19 (4): 260-263): glucose 10 grams, sodium chloride 0.3 gram, potassium chloride 0.3 gram, heptahydrate Magnesium sulfate 0.3 gram, ferrous sulfate heptahydrate 0.03 gram, manganese sulfate tetrahydrate 0.03 gram ammonium sulfate 0.5 gram, 15g agar, add distilled water to volume 1000 milliliters, adjust pH7.0, then add 0.5% tricalcium phos...

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Abstract

The invention discloses a Penicillium, a preparation method and applications thereof, Penicillium fungus PSM11-5 is separated from a vanadium ore sample; insoluble tricalcium phosphate, sodium metavanadate, cobalt hydroxide and basic nickel carbonate are taken as indicating compounds; and a fungal strain is screened by testing the capability of decomposing the tricalcium phosphate, the sodium metavanadate, the cobalt hydroxide and the basic nickel carbonate. The Penicillium PSM11-5 is Penicillium sp.PSM11-5 CCTCCM208207. The strain is utilized for carrying out biological leaching of phosphorus and biological metallurgy, metals of phosphorus, vanadium, nickel, cobalt and the like are leached from lean ores, discarded ores, submarginal ores, difficult-to-mine ores, difficult dressing ores and refractory ores, thereby fully utilizing the mineral resources, reducing the metallurgical costs and protecting the ecological environment. The PSM11-5 is utilized for leaching the phosphorus from low-grade phosphate rock powder, a biological fertilizer is prepared to be applied to the soil, thereby leading the soil to contain higher content of soluble phosphorus which can be utilized by crops; the strain further leaches insoluble phosphorus which is deposited in the soil before, thereby reducing phosphorus fertilizer and reducing gas pollution caused by the phosphorus fertilizer and water pollution caused by the phosphorus fertilizer.

Description

technical field [0001] The present invention relates to the microbial leaching of phosphorus and metals in phosphorus and metal ores, and more specifically relates to a penicillium fungus (Penicillium) which has a decomposing effect on insoluble phosphorus and metal ores, and also relates to the preparation method of the bacterium and the bacterium the use of. The Penicillium strain is a fungus that can leach phosphorus and metals from insoluble phosphate rocks and metal ores such as alum ore, nickel, cobalt ore, etc. The leaching substances are mainly organic acids such as gluconic acid. The strain is cultured in a more suitable leaching medium containing phosphate rock powder or metal ore powder, which can leach phosphorus and metals from minerals into the culture solution, so the strain can be used for the preparation or combination of phosphorus bacteria fertilizer Conventional techniques of hydrometallurgy are used industrially for microbial metallurgy. Background tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C22B3/18C01B25/01C12R1/80
CPCY02P10/20
Inventor 高梅影柴波刘朋明吴艳
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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