34 results about "Isoliquiritigenin" patented technology

The present invention relates to a composition of paeoniflorin and isoliquiritigenin and its application. The composition according to the present invention has rich raw materials, has effects such as anti-inflammatory, antioxidant, and inhibition of collagen deposition, can be effectively used for the treatment of diffuse pulmonary interstitial fibrosis, and has high economic and social benefits.

The invention relates to a qualitative and quantitative method for detecting active ingredients of root-strengthening and cough-relieving granules through UPLC-MS / MS, and belongs to the technical field of medicine analysis and detection. The four active ingredients of liquiritigenin, isoliquiritigenin, luteolin and bakuchiol in a sample can be detected at the same time. The method comprises the following steps: pretreating a sample by a 70% methanol ultrasonic extraction method to obtain a target sample solution, and detecting by using an SCIEX ExionLC AE high-pressure liquid phase system and an AB SCIEX 4500 mass spectrometer in combination with an MRM mode. Carrying out gradient elution on a liquid phase by using an HSS T3 C18 chromatographic column and 0.1% formic acid water-acetonitrile as a mobile phase; the mass spectrum is electrospray negative ion scanning, and a specific ion source and MRM parameters are set. The method can rapidly separate the four components, is short in detection period, high in sensitivity and strong in matrix interference resistance, can be accurately qualitative and quantitative, and provides support for preparation quality evaluation, pharmacodynamic mechanism research and clinical reasonable medication.

The invention discloses an application of a composition of isoliquiritigenin (glycoside), licochalcone and ethnic groups thereof in personal care products, and relates to the technical field of daily chemicals, and the composition is an anti-allergy soothing composition prepared based on a natural plant licorice extract isoliquiritigenin or a glycoside compound isoliquiritigenin thereof, licochalcone A and ethnic groups of licochalcone B, C, D, E and G. The traditional Chinese medicine composition has multiple functions of resisting allergy, relieving, resisting bacteria, diminishing inflammation, improving skin itch and stabbing pain and the like, does not contain hormones and antibiotics, has no toxic or side effect, is mild in effect and non-irritant to skin, can be used for treating skin allergy, red and swollen skin, itch and stabbing pain caused by various reasons, is applied to cosmetics and skin care products, such as gel, cream, masks and facial cleansers, and can be used for treating skin allergy, red and swollen skin, pruritus and stabbing pain caused by various reasons. The skin care product is mild to human bodies, safe and non-irritant, is suitable for sensitive skin, is suitable for people of all ages to use, has strong market competitiveness, and has relatively high economic and social benefits and the like.

The invention relates to a Ustilaginoidea virens botanical fungicide based on isoliquiritigenin. The invention develops a novel efficient, environment-friendly, safe and cost-controllable biopesticide mainly aiming at false smut by deeply researching the biological activity and action mechanism of plant source secondary metabolites, remarkably improves the field application effect and stability of the biopesticide by scientific proportioning and formula optimization, reduces the production cost and environmental pollution risk, and has a wide application prospect. Powerful support is provided for agricultural sustainable development and grain safety guarantee.

The invention provides a cough-relieving and phlegm-eliminating composition and a preparation method thereof. The cough-relieving and phlegm-eliminating composition is prepared from the following raw materials: anhydrous morphine, anhydrous codeine phosphate, apigenin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, trans-anethole, camphor and sodium benzoate. The composition further comprises a luffa stem extract, an atractylodes lancea rhizome extract and a radix ranunculi ternati bark extract. The cough-relieving and phlegm-eliminating composition provided by the invention has the advantages of few impurities, clear and remarkable drug effect and small side effect. Under the action of the core antitussive drug, three plant extracts of luffa stem, Atractylodes lancea and Ranunculus ternatus Thunb. Are creatively added, so that the phlegm eliminating capacity and the overall antitussive effect of the whole formula are remarkably enhanced. The cough-relieving and phlegm-eliminating composition disclosed by the invention realizes the synergistic interaction of '1 + 1 + 1gt, 3' on the core indexes of inhibiting the cough frequency, prolonging the cough latency period, promoting sputum excretion and the like, and realizes the cough-relieving and phlegm-eliminating effects of treating both symptoms and root causes through the multi-target-point and multi-mechanism synergistic effect.

A small molecule composition, comprising licochalcone A, licochalcone C, licochalcone D, licochalcone E, echinatin, isoliquiritigenin, formononetin, liquiritigenin, licoflavone C, glabrol, licoisoflavone A and licoisoflavone B, and the use thereof in preparing anticancer or antitumor drugs, anti-ferroptosis drugs, or drugs for sensitization of anticancer or antitumor drugs. The small molecule composition can also be used in combination with other antitumor drugs.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for simultaneously extracting and purifying glabridin, glycyrrhizic acid and isoliquiritigenin. The method comprises the following steps: (1) adding ethanol into glycyrrhiza glabra, and extracting to obtain an extracting solution; (2) extracting the extracting solution with ethyl acetate to obtain an organic phase and a water phase; and (3) purifying the organic phase by using a rapid preparative liquid chromatograph, carrying out gradient elution by using 30%-70% of ethyl acetate-cyclohexane as a mobile phase, and collecting the eluent, thereby obtaining the glabridin. (4) carrying out acidification and centrifugal precipitation on the water phase to obtain a glycyrrhizic acid crude product and a supernatant, adding absolute ethyl alcohol into the glycyrrhizic acid crude product, and carrying out ultrasonic extraction and centrifugation to obtain a glycyrrhizic acid ethanol solution; and (5) extracting and purifying the supernate to obtain the isoliquiritigenin.

The invention belongs to the technical field of biology, and particularly relates to application of astragaloside in regulation of parthenogenetic activation embryo development. According to the method disclosed by the invention, the maturation rate of the oocytes can be obviously improved by adding substances such as astragaloside into the sheep oocyte in-vitro maturation liquid. The preparation method comprises the following steps: firstly, respectively adding lycopene, astragaloside, isoliquiritigenin, forsythin and baicalein into an oocyte maturation solution, and screening out the optimal dosage of each additive according to the first polar body discharge rate, the cleavage rate and the blastocyst rate; and finally, adding an additive combination into the oocyte maturation liquid according to the screened optimal dosage, detecting the first polar body discharge rate, the cleavage rate and the blastocyst rate to determine an optimal composition, and detecting the levels of ROS, GSH, mitochondrial membrane potential, oxidative stress and apoptosis related genes of the optimal composition to further determine the application effect of the composition.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for simultaneously extracting and purifying glabridin, glycyrrhizic acid and isoliquiritigenin by a column chromatography extraction method. The method comprises the following steps: adding an ethanol solution into glycyrrhiza glabra, extracting by adopting a column chromatography extraction method, extracting an extracting solution by using dichloroethane, enabling an organic phase to pass through a silica gel column, and crystallizing an eluent to obtain glabridin. Acidifying and centrifuging the extracted water phase, and dissolving the precipitate with acetone to obtain glycyrrhizic acid; and extracting and purifying the filtrate to obtain the isoliquiritigenin. The invention provides a simple and efficient new extraction technology which is low in energy consumption, small in solvent dosage and high in extraction rate compared with traditional ultrasonic extraction and the like, and aims to extract glycyrrhizic acid and glabridin in glycyrrhiza glabra at the same time, separate glycyrrhizic acid and glabridin, separate isoliquiritigenin from the remaining part through simple conversion, and improve the purity of glycyrrhizic acid and glabridin in glycyrrhiza glabra. The comprehensive development and utilization of the glycyrrhiza glabra are achieved.

The invention provides an isoliquiritigenin-loaded drug-loaded liposome as well as a preparation method and application thereof, and relates to the technical field of drugs. The drug-loaded liposome is prepared from the following raw materials in parts by weight: 20-50 parts of lecithin, 6-15 parts of glycyrrhetinic acid and 2-10 parts of isoliquiritigenin, and can be used as a novel drug for treating androgenetic alopecia.

The invention belongs to the technical field of biology, and particularly relates to application of lycopene in sheep oocyte in-vitro culture. It is determined that the maturation rate of the oocytes can be remarkably increased by adding the lycopene and astragaloside composition into the sheep oocyte in-vitro maturation liquid. The preparation method comprises the following steps: firstly, respectively adding lycopene, astragaloside, isoliquiritigenin, forsythin and baicalein into an oocyte maturation solution, and screening out the optimal dosage of each additive according to the first polar body discharge rate, the cleavage rate and the blastocyst rate; and finally, adding an additive combination into the oocyte maturation liquid according to the screened optimal dosage, detecting the first polar body discharge rate, the cleavage rate and the blastocyst rate to determine an optimal composition, and detecting the levels of ROS, GSH, mitochondrial membrane potential, oxidative stress and apoptosis related genes of the optimal composition to further determine the application effect of the composition.

The invention belongs to the technical field of tobaccos, and particularly relates to a method for blocking generation of ash hanging tobaccos through a PPO activity inhibitor. According to the method, a PPO activity inhibitor is adopted for spraying or fumigating treatment before tobacco leaves are cured, so that the activity of PPO is inhibited, and ash-coated tobacco is prevented from being generated; the PPO active inhibitor is any one of Prothionamide (isopropylthioamide), Ruxolitinib (rucotinib) and isoliquiritigenin (isoliquiritigenin), and the PPO active inhibitor is any one of the following raw materials: isopropylthioicotinamide, ruxolitinib (rucotinib) and isoliquiritigenin (isoliquiritigenin). The method provided by the invention is applied to the production link of tobacco processing, the generation of ash-coated tobacco is reduced, and the product appearance and combustion quality are improved.

The invention belongs to the technical field of biological medicine, and discloses application of isoliquiritigenin as a polymyxin synergist. Through combination of isoliquiritigenin and polymyxin, it is found that isoliquiritigenin and polymyxin have a good effect on in-vivo and in-vitro salmonella inhibition. The isoliquiritigenin is safe in component, can achieve a good synergistic effect with the polymyxin, can significantly reduce the dosage of the polymyxin, is not easy to induce the generation of bacterial drug resistance, and has the characteristics of low price and good curative effect.

The invention discloses application of isoliquiritigenin in preparation of a medicine for treating 5-FU induced cardiotoxicity, and belongs to the technical field of biomedicine.The medicine comprises the isoliquiritigenin, and the isoliquiritigenin improves 5-FU induced oxidative stress and apoptosis by regulating an AhR-CYP1A1 pathway, so that the 5-FU induced cardiotoxicity is improved; the invention systematically reveals the action mechanism of the isoliquiritigenin for improving the 5-FU-induced cardiotoxicity by inhibiting an AhR-CYP1A1 signal channel for the first time from the animal overall level and the cell molecule level to mechanism analysis, and proves the application value of the isoliquiritigenin in preparation of the medicine for treating the 5-FU-induced cardiotoxicity; based on complete analysis of the mechanism, the invention provides a brand new active ingredient and a technical scheme for preparing the medicine for resisting 5-FU cardiotoxicity, lays a theoretical and experimental foundation for research and development of the medicine for relieving the side effect of 5-FU chemotherapy, and has an important medicine development value.

The invention discloses a method for screening anti-inflammatory heart protection active components of Siji decoction. The method comprises the following steps: 1, finding eicosanoic acid related to the drug effect of the Siji decoction and measuring the content of eicosanoic acid; 2, identification of components absorbed into blood by the Siji decoction and determination of relative peak intensity; and 3, analyzing the correlation between the content of the eicosanoic acid metabolite related to the drug effect of the Siji decoction and the relative peak intensity of the component absorbed into blood by the Siji decoction, and screening the anti-inflammatory heart protection active component of the Siji decoction. The method comprises the following steps: by taking an eicosanoic acid metabolic profile as a drug effect target, constructing a correlation model of quantitative information of eicosanoic acid metabolites in a rat with heart failure at different time points and blood-entering component intensities of Siji decoction at different time points; through screening, it is determined that the four components of songorine, neoline, talasamine and isoliquiritigenin have the definite anti-inflammatory heart protection effect, and the method is accurate and efficient.

A cosmetic composition is disclosed comprising i) a scalp health agent; and ii) a natural active selected from cotoin, isoliquiritigenin, xanthohumol, pinostrobin chalcone, and a mixture thereof; wherein the scalp health agent is selected from a group consisting of polyvalent metal salts of pyrithione, hydroxamic acid, hinokitiol, azole compound and selenium sulfide; wherein the weight ratio of the amount of said active to that of said scalp health agent is from 1:100 to 100:1.

The application discloses a kind of based on isoliquiritigenin biological base temperature-resistant antibacterial foam cotton material and preparation method and application thereof, the foam cotton material is obtained by foaming based on the main chain type benzoxazine resin of isoliquiritigenin biological base, with maleic anhydride, foaming agent master batch, the main chain type benzoxazine resin of based on isoliquiritigenin biological base molecular formula as shown below.The application is prepared with the main chain type benzoxazine resin of isoliquiritigenin biological base as organism to obtain the benzoxazine foam with temperature-resistant antibacterial property of body.The foam cotton material of the application has the following advantages: based on the molecular structure of isoliquiritigenin, product has excellent antibacterial property;High crosslinking density benzoxazine gives product excellent temperature resistance;The product thermal conductivity is as low as 0.033W / (m·K), and the product has wide application in medical equipment, electronic equipment, new energy automobile and other fields.

The invention relates to the technical field of strain screening, in particular to a method for rapidly screening isoliquiritigenin transformed strains on a large scale on the basis of MTHM, a microplate reader is adopted for rapid screening, a transformed product is verified through thin-layer chromatography, high performance liquid chromatography and liquid mass spectrometry, the detection precision is amplified step by step, and the isoliquiritigenin transformed strains can be obtained. The screening efficiency of the isoliquiritigenin transforming bacteria is obviously improved. The method not only is efficient and reliable, but also provides a new technical thought and theoretical basis for development of natural products and application of microbial conversion.

The invention provides application of isoliquiritigenin in relieving toxicity of pinellia ternate and a pinellia ternate detoxification medicine, and belongs to the technical field of biology. The invention proves that the ISL can inhibit the expression increase of TNF-alpha and IL-1beta mRNA caused by pinellia ternate poison needle crystals and PTL, inhibit the increase of ROS in macrophages caused by pinellia ternate poison needle crystals, and weaken inflammatory response caused by pinellia ternate poison needle crystals. According to the present invention, the ISL can inhibit the oxidative stress reaction caused by PTL by inhibiting the combination of PTL and the macrophage membrane, and inhibit the excessive generation of ROS in the macrophage so as to block the MAPK inflammation pathway activation, such that the activation of the JAK-STAT signal pathway is inhibited, the secretion of inflammatory factors TNF-alpha, IL-1beta and IL-6 is reduced, the inflammatory cascade reaction process is blocked, and the inflammatory reaction degree is significantly weakened;

The present invention relates to a composition of paeoniflorin and isoliquiritigenin and application thereof. The composition according to the present invention is rich in raw materials, and has the effects of anti-inflammation, antioxidant, inhibiting collagen deposition, etc., and can be effectively used to treat diffuse interstitial pulmonary fibrosis with high economic and social benefits.

This invention belongs to the field of biomedical technology, specifically relating to an isoliquiritigenin composition for improving depression and its preparation method. The isoliquiritigenin composition for improving depression comprises the following raw materials in parts by weight: 22-40 parts isoliquiritigenin, 10-17 parts N-acetyl-D-glucosamine, and 15-20 parts β-lapaquinone. Isoliquiritigenin, N-acetyl-D-glucosamine, and β-lapaquinone constitute a multi-target synergistic isoliquiritigenin composition for improving depression. Experimental results show that by taking the isoliquiritigenin composition prepared according to this invention, it can systematically exert its effects and comprehensively improve the pathological state of depression, showing broad application prospects.

The invention discloses a preparation method of isoliquiritin and artificially synthesized liquiritin, which is characterized by comprising the following steps: carrying out aldol condensation reaction on 2, 4-dihydroxyacetophenone of which hydroxyl is protected by acyl and 4-hydroxybenzaldehyde to obtain acyl-protected isoliquiritigenin as a precursor; the preparation method comprises the following steps: carrying out glycosylation reaction on a precursor and an acyl-protected glucosyl donor in the presence of an accelerant to obtain acyl-protected isoliquiritin, then carrying out deprotection to obtain isoliquiritin and artificially synthesized liquiritin at the same time, and further carrying out alkali catalysis ring closing on the isoliquiritin to obtain the artificially synthesized liquiritin. Compared with a method for directly extracting liquiritin and isoliquiritin from licorice roots in the prior art, the method has very obvious technical and industrial advantages, raw materials are cheap and easy to obtain, reaction conditions are mild, product purity is high, the defects that an existing isoliquiritin synthesis method is low in yield, large in workload and the like are overcome, artificially synthesized liquiritin can be further obtained, and the method is suitable for industrial production. The method has a very good industrialization prospect and a great prospect of modernization of traditional Chinese medicines through chemical modification and transformation.

The invention provides a detection method for simultaneously determining the contents of 12 components in a dragon's blood medicinal material, which adopts a high performance liquid chromatography method for simultaneously determining the contents of 7, 8-dihydroxy-7, 8-dihydroxy-7, 8-dihydroxy-7, 8-dihydroxy-7 the content of 4, 4 '-dihydroxy flavone, resveratrol, loureirin A, loureirin B, loureirin C, loureirin D, pterostilbene, pinoresinol, licochalcone, 7, 4'-dihydroxy homoisoflavone, liquiritigenin and isoliquiritigenin is measured, and mobile phases comprise a mobile phase A glacial acetic acid aqueous solution and a mobile phase B acetonitrile; the method comprises the following steps: S1, preparing a test solution; s2, preparing a mixed standard reference solution; and S3, high performance liquid chromatography determination. The detection method established by the invention can simultaneously detect 12 medicinal components in the dragon's blood medicinal material, and can provide a basis for quality evaluation and control of the dragon's blood medicinal material. The detection method is stable, feasible, reasonable in design, high in operability, precise and reliable.

The invention discloses an iron-based-isoliquiritigenin nano-enzyme as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The preparation method comprises the following steps: dissolving polyvinylpyrrolidone and ferric salt in a solvent, and carrying out a mixed reaction to obtain a reaction solution; and dissolving isoliquiritigenin, adding the isoliquiritigenin into the reaction solution, continuously reacting, and purifying to obtain the iron-based-isoliquiritigenin nano-enzyme. In view of the fact that traumatic brain injury is often accompanied by severe oxidative stress and neuroinflammation reaction, the invention provides a new application of the iron-based-isoliquiritigenin nano-enzyme in preparation of drugs for treating traumatic brain injury. The iron-based-isoliquiritigenin nano-enzyme which is stable in structure and clear in function developed by the invention not only expands the application of the nano-enzyme in the field of biomedicine, but also provides a brand new path for developing a novel medicament for treating traumatic brain injury.

The invention discloses an application of isoliquiritigenin and / or glabridin in preparation of a medicine for treating acanthamoeba infection. The research finds that the isoliquiritigenin and the glabridin are adopted to treat the acanthamoeba trophoblast cells, proliferation of the acanthamoeba trophoblast cells can be remarkably inhibited, toxicity to host cells is small, and the inhibition effect of the isoliquiritigenin and the glabridin on the acanthamoeba is more remarkable when the isoliquiritigenin and the glabridin are matched. Therefore, the isoliquiritigenin and / or glabridin can be used for preparing medicines or preparations for preventing and / or treating diseases caused by acanthamoeba infection, removing acanthamoeba cells or inhibiting acanthamoeba cell proliferation.

The invention discloses an isoliquiritigenin preparation method combining enzyme inhibition and peeling treatment, and relates to the technical field of natural product extraction. Enzyme deactivation treatment and peeling processes are innovatively introduced, so that enzymatic browning and pigment migration are inhibited from the source, the color depth of isoliquiritigenin is effectively reduced, the product is bright yellow, and the quality of isoliquiritigenin is improved. Meanwhile, due to the optimized decolorizing effect, the use of a decolorizing agent is reduced, the production efficiency is greatly improved, the cost is reduced, the environmental protection property and the industrial feasibility of the process are ensured, and compared with a traditional process, the process disclosed by the invention not only simplifies the flow, improves the product purity, but also remarkably enhances the market competitiveness; and a technical guarantee is provided for realizing green and efficient production and internationalized expansion of enterprises.

The invention relates to the technical field of medicines, in particular to application of isoliquiritin in preparation of medicines for preventing and treating mustard gas nerve injury. The invention also provides an application of isoliquiritin in preparation of neuroprotective drugs. The isoliquiritin exerts effective neuroprotective activity on mustard gas injury, can remarkably improve the situation that the survival rate of PC12 cells is reduced due to mustard gas, remarkably inhibits LDH outflow and ROS rising of the cells, and relieves cell DNA injury caused by mustard gas. At the animal level, mouse brain tissue neuronal degeneration and nissl body reduction caused by mustard gas are improved, and the spontaneous activity condition of mouse brain tissue neuronal degeneration and nissl body reduction are improved. The invention provides a new reference way for treating mustard gas nerve injury, and the isoliquiritin has a prospect of preparing a neuroprotective agent aiming at mustard gas.

The invention relates to an application of isoliquiritigenin in preparation of chronic wound medicines or medical instruments. The invention also provides a nano drug delivery system which is covalent organic framework nano particles (ISL (at) bCOF) loaded with isoliquiritigenin. The COF carrier is connected with an ISL molecule through a boron-oxygen bond, and a'gating 'system sensitive to a wound surface high-glucose microenvironment is constructed. In a normal physiological environment, the boron-oxygen bond is stable, and the drug release is slow; when in a high-glucose environment of a chronic wound surface, the boric acid ester bond is broken, so that the ISL is quickly and specifically released and acts on macrophages at the wound surface in a targeting manner. Meanwhile, by matching with a high-strength hydrogel microneedle substrate, the prepared microneedle has excellent mechanical strength, can be ensured to be enough to pierce a skin cuticle, has good biocompatibility and high permeability, and is convenient to package and deliver the nano drug delivery system.

The invention discloses a hematoxyloxymethyltransferase CsOMT80 mutant and an application of the hematoxyloxymethyltransferase CsOMT80 mutant in synthesis of chalcone compounds. Through structural biological modeling and functional site analysis, on the basis of hematoxylon multi-omics data and identification, an oxygen methyltransferase coding gene CsOMT80 closely related to methoxychalcone synthesis is obtained, structural prediction and molecular docking are performed on an amino acid sequence of the oxygen methyltransferase coding gene CsOMT80, and key sites influencing substrate binding and catalytic efficiency are screened out. On the basis, amino acid replacement is carried out on a target site by adopting a site-directed mutagenesis strategy, and a plurality of mutants are constructed and obtained. Functional verification finds that the mutant CsOMT80G112R shows remarkably enhanced catalytic efficiency when a catalytic reaction is carried out by taking isoliquiritigenin as a substrate, and the enzymatic activity of the mutant CsOMT80G112R is 1278 times that of a wild type and is superior to that of reported O-methyltransferase (ChOMT) from alfalfa.