25 results about "Liquiritigenin" patented technology

The invention discloses a method and system for predicting the effect of repairing lung injury by isoliquiritigenin based on machine learning, and relates to the field of drug effect prediction.The method comprises the steps that biomarker data and historical experiment repairing effect data are obtained; on the basis of molecular structure data and biomarker data, calculating a molecular interaction force index and a cell permeability index of the isoliquiritigenin, and analyzing through a multiple regression analyzer in combination with historical experiment repair effect data to obtain a repair effect coefficient; according to the repair effect coefficient, configuring an effect confidence interval, and performing range optimization on the molecular interaction force index and the cell permeability index to obtain an optimized biological activity index; and constructing an effect prediction model based on a neural network, inputting the optimized biological activity indexes into the effect prediction model, and outputting to obtain a lung injury repair effect prediction value. The problems that the prediction precision is insufficient and the prediction is too one-sided due to the lack of comprehensive analysis on a molecular mechanism and a biomarker are solved.

The invention discloses a platelet increasing capsule characteristic chromatogram and a construction method and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine compound preparations. According to the method, 13 control components including gallic acid, catechin, paeoniflorin, forsythiaside B, benzoic acid, astragalus smicus glycoside, forsythin, arctiin, liquiritigenin, benzoyl paeoniflorin, paeonol, arctigenin and indirubin are selected, a high performance liquid chromatography-mass spectrometry technology is applied, multiple batches of preparation samples are analyzed, and the content of the components in the preparation samples is determined. And establishing a characteristic spectrum of the chemical components. Compared with a traditional method, the technology has good precision and reproducibility, the data result is accurate and reliable, meanwhile, the technology can identify the 13 components at the same time under the same chromatographic condition, the analysis efficiency is remarkably improved, synchronous monitoring of multiple effective components is achieved, and the consistency and stability of the preparation quality can be guaranteed.

The invention provides a method for simultaneously separating three flavone monomeric compounds from glycyrrhiza glabra, and belongs to the technical field of compound separation. The method comprises the following steps: mixing glycyrrhiza glabra powder with ethanol, carrying out supercritical CO2 extraction, carrying out spin-drying, mixing an extract and ethanol, carrying out ultrasonic dissolution and standing in sequence, adding a supernatant fraction into a macroporous resin chromatographic column from the upper part, eluting the macroporous resin chromatographic column with ethanol, and carrying out vacuum concentration to obtain the glycyrrhiza glabra extract. Desolventizing the eluted fraction to obtain a main fraction extract; mixing the main fraction extract with a recrystallization solvent, and standing the mixed solution to obtain a solid 1 and a filtrate 1; standing the filtrate 1 to obtain a solid 2 and filtrate 2; standing the filtrate 2 to obtain a solid 3; according to the method, the extraction efficiency is high, the supercritical CO2 has low viscosity, high diffusivity, high mass transfer rate, strong permeability and relatively short extraction time; according to the method disclosed by the invention, three kinds of glycyrrhizic flavonoid compounds, namely glabridin, glabridin and isopentenyl coumestrol, are successfully obtained.

The invention discloses a Sorosil decoction specific chromatogram and a construction method and application thereof, and belongs to the technical field of traditional Chinese medicine analysis and detection. The method comprises the following steps: respectively dissolving reference substances of bergenin, arbutin, glycyrrhizic acid, liquiritin, liquiritigenin and gallic acid in a solvent to obtain reference substance solutions; adding soroxil decoction into a solvent, carrying out ultrasonic extraction, and filtering to obtain a test solution; and injecting the reference solution and the test solution into a high performance liquid chromatograph for detection, taking the specific chromatogram of the reference solution as a reference chromatogram, selecting common peaks from the specific chromatogram of the test solution, and constructing the specific chromatogram of the soroxil decoction. The invention also discloses a Sorosil decoction characteristic spectrum and application thereof. According to the specific chromatogram of the soroxi decoction, rapid identification of the formula of the soroxi decoction can be realized, the method is simple, the research blank of the specific chromatogram in quality control of the classic famous-prescription soroxi decoction and the preparation thereof is filled up, and a powerful guarantee is provided for quality improvement of the classic famous-prescription soroxi decoction.

The invention provides a cough-relieving and phlegm-eliminating composition and a preparation method thereof. The cough-relieving and phlegm-eliminating composition is prepared from the following raw materials: anhydrous morphine, anhydrous codeine phosphate, apigenin, liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, trans-anethole, camphor and sodium benzoate. The composition further comprises a luffa stem extract, an atractylodes lancea rhizome extract and a radix ranunculi ternati bark extract. The cough-relieving and phlegm-eliminating composition provided by the invention has the advantages of few impurities, clear and remarkable drug effect and small side effect. Under the action of the core antitussive drug, three plant extracts of luffa stem, Atractylodes lancea and Ranunculus ternatus Thunb. Are creatively added, so that the phlegm eliminating capacity and the overall antitussive effect of the whole formula are remarkably enhanced. The cough-relieving and phlegm-eliminating composition disclosed by the invention realizes the synergistic interaction of '1 + 1 + 1gt, 3' on the core indexes of inhibiting the cough frequency, prolonging the cough latency period, promoting sputum excretion and the like, and realizes the cough-relieving and phlegm-eliminating effects of treating both symptoms and root causes through the multi-target-point and multi-mechanism synergistic effect.

A small molecule composition, comprising licochalcone A, licochalcone C, licochalcone D, licochalcone E, echinatin, isoliquiritigenin, formononetin, liquiritigenin, licoflavone C, glabrol, licoisoflavone A and licoisoflavone B, and the use thereof in preparing anticancer or antitumor drugs, anti-ferroptosis drugs, or drugs for sensitization of anticancer or antitumor drugs. The small molecule composition can also be used in combination with other antitumor drugs.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for simultaneously extracting and purifying glabridin, glycyrrhizic acid and isoliquiritigenin. The method comprises the following steps: (1) adding ethanol into glycyrrhiza glabra, and extracting to obtain an extracting solution; (2) extracting the extracting solution with ethyl acetate to obtain an organic phase and a water phase; and (3) purifying the organic phase by using a rapid preparative liquid chromatograph, carrying out gradient elution by using 30%-70% of ethyl acetate-cyclohexane as a mobile phase, and collecting the eluent, thereby obtaining the glabridin. (4) carrying out acidification and centrifugal precipitation on the water phase to obtain a glycyrrhizic acid crude product and a supernatant, adding absolute ethyl alcohol into the glycyrrhizic acid crude product, and carrying out ultrasonic extraction and centrifugation to obtain a glycyrrhizic acid ethanol solution; and (5) extracting and purifying the supernate to obtain the isoliquiritigenin.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a method for simultaneously extracting and purifying glabridin, glycyrrhizic acid and isoliquiritigenin by a column chromatography extraction method. The method comprises the following steps: adding an ethanol solution into glycyrrhiza glabra, extracting by adopting a column chromatography extraction method, extracting an extracting solution by using dichloroethane, enabling an organic phase to pass through a silica gel column, and crystallizing an eluent to obtain glabridin. Acidifying and centrifuging the extracted water phase, and dissolving the precipitate with acetone to obtain glycyrrhizic acid; and extracting and purifying the filtrate to obtain the isoliquiritigenin. The invention provides a simple and efficient new extraction technology which is low in energy consumption, small in solvent dosage and high in extraction rate compared with traditional ultrasonic extraction and the like, and aims to extract glycyrrhizic acid and glabridin in glycyrrhiza glabra at the same time, separate glycyrrhizic acid and glabridin, separate isoliquiritigenin from the remaining part through simple conversion, and improve the purity of glycyrrhizic acid and glabridin in glycyrrhiza glabra. The comprehensive development and utilization of the glycyrrhiza glabra are achieved.

The invention belongs to the field of analysis of traditional Chinese medicine preparations, and particularly discloses a method for determining the content of 15 components in a Xiaoyao preparation through quantitative analysis of multi-components by single marker and application of the method. According to the invention, pentagalloylglucose is used as an internal reference substance for quantitative analysis of multi-components by single marker; relative correction factors between the pentagalloylglucose and gallic acid, chlorogenic acid, albiflorin, paeoniflorin, schaftoside, ferulic acid, apiose liquiritin, liquiritin, senkyunolide I, paeoniflorin, senkyunolide H, formononin, liquiritigenin and glycyrrhizic acid in the Xiaoyao preparation are established, and the contents of the 15 components in the Xiaoyao preparation are calculated through the correction factors. The method disclosed by the invention is high in practicability, simple to operate, cost-saving and accurate and reliable in detection result, overcomes the defects of the quality detection method of the Xiaoyao preparation, especially the Xiaoyao mixture, and provides a new way for quality control of the Xiaoyao preparation and internal quality evaluation of medicines.

The invention discloses an application of liquiritigenin in preparation of a bactericide or an antibiotic synergist. The invention discloses the synergistic effect, the antibacterial property and the synergistic mechanism of liquiritigenin on colistin and the potential value of in-vivo combined application of liquiritigenin. On the basis, a feasible treatment scheme is provided to cope with the increasingly aggravated drug resistance problem, the use curative effect of the colistin is effectively enhanced, and the clinical service life of the colistin is prolonged. The possibility that liquiritigenin is used as a colistin synergist to resist infection of multi-drug-resistant pathogenic bacteria is reduced. The liquiritigenin can reduce the minimum inhibitory concentration of the colistin to drug-resistant gram-negative bacteria by 16 times, and the antibacterial activity of the colistin is greatly improved.

The invention relates to the technical field of traditional Chinese medicine, in particular to a composition with a qi-blood tonifying effect and a pharmaceutical preparation and application thereof. The composition is prepared from the following raw materials in parts by weight: 0.2 to 1 part of tangeretin, 0.2 to 5 parts of nobiletin, 0.05 to 0.2 part of lobetyolin, 0.1 to 1 part of allantoin, 0.1 to 1 part of liquiritigenin, 0.1 to 1 part of alnus ketone, 0.02 to 0.2 part of rutin, 0.2 to 2 parts of apiose liquiritin and 0.1 to 1 part of ammonium glycyrrhizinate. The traditional Chinese medicine composition has the remarkable effects of replenishing blood and qi, treating anemia and resisting fatigue.

The invention discloses application of isoliquiritigenin in preparation of a medicine for treating 5-FU induced cardiotoxicity, and belongs to the technical field of biomedicine.The medicine comprises the isoliquiritigenin, and the isoliquiritigenin improves 5-FU induced oxidative stress and apoptosis by regulating an AhR-CYP1A1 pathway, so that the 5-FU induced cardiotoxicity is improved; the invention systematically reveals the action mechanism of the isoliquiritigenin for improving the 5-FU-induced cardiotoxicity by inhibiting an AhR-CYP1A1 signal channel for the first time from the animal overall level and the cell molecule level to mechanism analysis, and proves the application value of the isoliquiritigenin in preparation of the medicine for treating the 5-FU-induced cardiotoxicity; based on complete analysis of the mechanism, the invention provides a brand new active ingredient and a technical scheme for preparing the medicine for resisting 5-FU cardiotoxicity, lays a theoretical and experimental foundation for research and development of the medicine for relieving the side effect of 5-FU chemotherapy, and has an important medicine development value.

The invention discloses a method for screening anti-inflammatory heart protection active components of Siji decoction. The method comprises the following steps: 1, finding eicosanoic acid related to the drug effect of the Siji decoction and measuring the content of eicosanoic acid; 2, identification of components absorbed into blood by the Siji decoction and determination of relative peak intensity; and 3, analyzing the correlation between the content of the eicosanoic acid metabolite related to the drug effect of the Siji decoction and the relative peak intensity of the component absorbed into blood by the Siji decoction, and screening the anti-inflammatory heart protection active component of the Siji decoction. The method comprises the following steps: by taking an eicosanoic acid metabolic profile as a drug effect target, constructing a correlation model of quantitative information of eicosanoic acid metabolites in a rat with heart failure at different time points and blood-entering component intensities of Siji decoction at different time points; through screening, it is determined that the four components of songorine, neoline, talasamine and isoliquiritigenin have the definite anti-inflammatory heart protection effect, and the method is accurate and efficient.

This invention belongs to the field of biomedical technology, specifically relating to an isoliquiritigenin composition for improving depression and its preparation method. The isoliquiritigenin composition for improving depression comprises the following raw materials in parts by weight: 22-40 parts isoliquiritigenin, 10-17 parts N-acetyl-D-glucosamine, and 15-20 parts β-lapaquinone. Isoliquiritigenin, N-acetyl-D-glucosamine, and β-lapaquinone constitute a multi-target synergistic isoliquiritigenin composition for improving depression. Experimental results show that by taking the isoliquiritigenin composition prepared according to this invention, it can systematically exert its effects and comprehensively improve the pathological state of depression, showing broad application prospects.

The invention belongs to the technical field of traditional Chinese medicine preparation component detection, and particularly relates to a method for detecting the content of main active components in ginger and liquorice decoction. According to the content detection method disclosed by the invention, flavonoid, triterpenoid saponin and phenol components in the ginger and liquorice decoction are detected by adopting an HPLC (High Performance Liquid Chromatography). Through specific sample pretreatment and chromatographic conditions, main active ingredients such as liquiritin, apiose isoliquiritin, isoliquiritin, liquiritigenin, mono-ammonium glycyrrhizinate and 6-gingerol in the main medicines ginger and liquorice in the ginger and liquorice decoction can be quantitatively detected at the same time, so that the clinical efficacy of the ginger and liquorice decoction preparation is guaranteed; and a more comprehensive method is provided for quality control of the ginger and licorice root soup.

The invention discloses an application of isoliquiritigenin and / or glabridin in preparation of a medicine for treating acanthamoeba infection. The research finds that the isoliquiritigenin and the glabridin are adopted to treat the acanthamoeba trophoblast cells, proliferation of the acanthamoeba trophoblast cells can be remarkably inhibited, toxicity to host cells is small, and the inhibition effect of the isoliquiritigenin and the glabridin on the acanthamoeba is more remarkable when the isoliquiritigenin and the glabridin are matched. Therefore, the isoliquiritigenin and / or glabridin can be used for preparing medicines or preparations for preventing and / or treating diseases caused by acanthamoeba infection, removing acanthamoeba cells or inhibiting acanthamoeba cell proliferation.

To develop materials that effectively produce anti-inflammatory effects. [Solution] A composition containing glycyrrhizic acid, liquiritin, and isoliquiritin, The mass ratio of glycyrrhizic acid to liquiritin (glycyrrhizic acid:liquiritin) is 1:0.3 to 2. The mass ratio of glycyrrhizic acid to isoliquiritin (glycyrrhizic acid:isoliquiritin) is 1:0.5 to 3. composition.

The invention relates to the technical field of traditional Chinese medicine, and particularly discloses a traditional Chinese medicine monomer composition for chronic renal failure and application thereof. The traditional Chinese medicine monomer composition is prepared from the following raw materials in molar ratio: 9.5 to 40 mol of liquiritigenin, 3.5 to 9.5 mol of ginsenoside Rg2, 1 to 4 mol of quercetin, 0.5 to 3.5 mol of tanshinone IIA and 1 to 5 mol of baicalin. The invention further discloses application of the traditional Chinese medicine monomer composition in preparation of a preparation for chronic renal failure and application of the traditional Chinese medicine monomer composition in preparation of a medicine for chronic renal failure. The traditional Chinese medicine monomer composition has good in-vivo anti-inflammatory and anti-renal fibrosis activity, can significantly improve the renal function of chronic renal failure animals, and has a good application prospect.

The invention relates to the technical field of microbial mutagenesis, and particularly provides a method for ultraviolet-induced synergism of isoliquiritigenin transformed bacteria, which comprises the following steps: a bacterial suspension of the isoliquiritigenin transformed bacteria is irradiated by ultraviolet light, the height of the ultraviolet light irradiation is 5-10cm, and the intensity is 40-60%. According to the method, mutation of microbial genomes is induced through ultraviolet irradiation, then the metabolic pathway of the microbial genomes is changed, the transformation efficiency of the strains can be effectively improved through targeted limitation on irradiation height and intensity, and the method is convenient to popularize.

The invention provides a preparation method of magnesium isoglycyrrhizinate, and belongs to the technical field of raw material medicine preparation, the preparation method comprises the following steps: taking glycyrrhetinic acid as an initial raw material, carrying out isomerization reaction under an alkaline condition, then recrystallizing to obtain isoglycyrrhetinic acid, and recovering glycyrrhetinic acid; carrying out glucuronization reaction on the isoglycyrrhetinic acid under the action of enzyme to generate isoliquiritigenin, and carrying out salt forming reaction on the isoglycyrrhetinic acid to obtain the magnesium isoglycyrrhizinate. The recovered glycyrrhetinic acid is used as an initial raw material again to prepare the magnesium isoglycyrrhizinate. A brand new magnesium isoglycyrrhizinate preparation route is designed and implemented, no literature report exists at present, glycyrrhetinic acid is adopted as a raw material, the influence of glucuronic acid groups on the refining process is eliminated, the refining efficiency is high, and the molar yield is high.

The invention discloses a Ganhai Weikang capsule specific chromatogram based on a multi-component material basis as well as a construction method and application thereof, and belongs to the technical field of quality control and evaluation of Chinese patent medicines. A high performance liquid chromatography-high resolution mass spectrometry method is adopted for detection, and glycyrrhizic acid, liquiritin, liquiritigenin, apiose liquiritin, naringenin, chlorogenic acid, syringic acid, protocatechuic acid, hesperidin, narirutin, p-coumaric acid and kaempferide are taken as reference substances; the specific chromatogram is successfully constructed by detecting different batches of Ganhai Weikang capsules. The method for detecting the specific chromatogram of the Ganhai Weikang capsule, established by the invention, is simple and convenient to operate, high in precision and rapid in analysis, can realize efficient and synchronous detection of 12 characteristic components, and has good stability, accuracy and reproducibility. According to the method, synchronous and rapid analysis of multiple components is realized, the detection efficiency is remarkably improved, and reliable scientific basis and technical support are provided for quality control and clinical curative effect guarantee of the Ganhai Weikang capsules.

The invention relates to an application of isoliquiritigenin in preparation of chronic wound medicines or medical instruments. The invention also provides a nano drug delivery system which is covalent organic framework nano particles (ISL (at) bCOF) loaded with isoliquiritigenin. The COF carrier is connected with an ISL molecule through a boron-oxygen bond, and a'gating 'system sensitive to a wound surface high-glucose microenvironment is constructed. In a normal physiological environment, the boron-oxygen bond is stable, and the drug release is slow; when in a high-glucose environment of a chronic wound surface, the boric acid ester bond is broken, so that the ISL is quickly and specifically released and acts on macrophages at the wound surface in a targeting manner. Meanwhile, by matching with a high-strength hydrogel microneedle substrate, the prepared microneedle has excellent mechanical strength, can be ensured to be enough to pierce a skin cuticle, has good biocompatibility and high permeability, and is convenient to package and deliver the nano drug delivery system.

The invention provides a quality control method of a traditional Chinese medicine compound for gastric ulcer. The method comprises the following steps: using a calycosin reference substance, an isoliquiritigenin reference substance, a ceratin reference substance, a formononetin reference substance, a parthenolide reference substance, an atractylenolide I reference substance, an atractylenolide II reference substance, an isohesperidin reference substance, a tyrosine reference substance, an atractylenolide III reference substance and a cryptochlorogenic acid reference substance; and preparing a mixed reference substance solution from the honeysuckle glycoside reference substance, and determining the contents of the 12 components in the traditional Chinese medicine solution to be tested. The invention establishes a traditional Chinese medicine compound quality control method for gastric ulcer, and the method can be applied to quality control of a Chinese patent medicine monkshood middle-jiao-regulating pill.

The invention belongs to the technical field of biology, and discloses application of isoliquiritigenin in preparation of a product for preventing and / or treating fish infected with a siniperca chuatsi and frog iridovirus (MRV), the EC50 of the isoliquiritigenin is 3.08 mu M, and the isoliquiritigenin has a remarkable in-vivo and in-vitro anti-MRV effect, so that the treatment administration effect is the best, the premixing administration is the second time, and the effect of preventing and / or treating the fish infected with the siniperca chuatsi and frog iridovirus is achieved. The result shows that the isoliquiritigenin possibly has a direct damage effect on MRV virus particles and inhibits MRV virus proliferation; besides, excessive inflammation and cell apoptosis caused by MRV infection can cause fish tissue damage and can promote virus proliferation and escape, and isoliquiritigenin can relieve tissue damage caused by MRV virus, inhibit virus proliferation and play a comprehensive antiviral role through anti-inflammatory and anti-apoptosis effects; meanwhile, the invention also provides a medicine containing isoliquiritigenin, a fish feed and a fish feed additive.