72 results about "Schaftoside" patented technology

An anti-aging composition comprising: (i) at least one biomimetic oligopeptide having a sequence of 20 amino acids or less; (ii) at least one lipoaminoacid; (iii) at least one pentacyclic triterpenoid selected from the group consisting of asiaticoside, madecassic acid, asiatic acid and madecassoside; (iv) at least one antioxidant; and (v) tetrahydropiperine; a kit comprising such composition and a method of use.

The invention relates to a quality evaluation method for a dendrobe medicinal material. The method includes the steps that a schaftoside chromatographic peak is adopted as a reference substance, and a standard fingerprint spectrum is formulated through HPLC (high performance liquid chromatography); through comparison of fingerprint spectrums of samples for test and the standard fingerprint spectrum, the quality of the samples for test is determined. According to the method, the pretreatment method for all the samples for test is simple, characteristic components are reserved, and the solution stability of the samples for test is good. The high performance liquid chromatography is high in precision, good in reproducibility and short in analysis time, and has certain specificity, the separation effect of all fingerprint peaks in the obtained fingerprint spectrum is good, the quality of the dendrobe medicinal material can be accurately and comprehensively reflected, the quality standard of the dendrobe medicinal material is further perfected, and therefore the quality evaluation method can be applied to quality grading and authenticity of dendrobe tissue culture seedlings, fresh dendrobe and air-dried dendrobe of medicinal dendrobe.

The invention discloses a method for preparing schaftoside from desmodium styracifolium. The method comprises the following steps of: i, crushing the desmodium styracifolium, adding biological enzyme and uniformly stirring, performing enzymolysis at normal temperature for 4-7 days, soaking enzymolysis raw material with warm water and extracting for two to three times, filtering extract and ultra-filtering with an ultrafiltration membrane, concentrating permeate with a nanofiltration membrane, extracting the concentrate by using acetic ether and concentrating the extract to obtain a rough extract; and ii, separating the rough extract by using high-speed counter-current chromatography, performing on-line monitoring through an ultraviolet detector, collecting a target component and performing reduced pressure drying to obtain the schaftoside. The method has the advantages of simple operation, high efficiency, large preparation capacity and high purity of an obtained product.

The invention belongs to the technical field of traditional Chinese medicine sustained-release preparations, in particular to a water-soluble effective component containing centella asiatica for treating trauma, surgical trauma, burns, keloids and scleroderma, antidepressant and treating liver and kidney fibrosis. Centella glycosides) traditional Chinese medicine slow-release preparation and preparation method thereof. The invention adopts the modern preparation technology to compress the traditional Chinese medicine slow-release preparation with solid medicine, skeleton-type auxiliary materials and other excipients. The solid drug refers to the total glycosides of the water-soluble extract of the traditional Chinese medicine Centella asiatica. The total glycosides contain madecassoside and centella asiatica. The total glycosides content in the extract should not be less than 60%. Skeleton-type excipients mainly refer to hydroxypropyl methylcellulose. By adjusting the amount of excipients in the prescription, the release rate of asiaticoside can be controlled, so as to stabilize the blood concentration of the drug, reduce the frequency of medication, and facilitate the use of patients.

The invention provides a method for purifying schaftoside and in particular relates to a method for purifying schaftoside from snowbellleaf tickclover herb. The method comprises the following steps: grinding snowbellleaf tickclover herb, adding 5% of sodium bicarbonate solution for heating extraction, adding extract to be acidic, adding water saturated n-butanol for extraction, using extract to recover n-butanol to obtain dried matters and then separating the dried matters by high-speed counter-current chromatography to obtain high-content schaftoside. The method has the following advantages: the high-speed counter-current method is adopted to purify schaftoside; the process has strong specificity; and the product has high yield and purity.

The invention provides a method for measuring the content of vicenin-1, vicenin-3 and schaftoside in desmodium styracifolium medicinal materials, a total flavonoid extract from desmodium styracifolium and a preparation thereof at the same time by using a single reference substance, namely the schaftoside. The method comprises the steps that the schaftoside in total flavonoid extract from desmodium styracifolium or the preparation is taken as an internal standard substance, wherein the content of effective components of the schaftoside is high and easy to be obtained; the relative correction factor of the schaftoside and the vicenin-1 and the vicenin-3 is calculated, and the relative correction factor is taken as a constant, the content of the vicenin-1, the vicenin-3 and the schaftoside in the total flavonoid extract from desmodium styracifolium can be measured at the same time, the quality of the total flavonoid extract from desmodium styracifolium or the preparation in industrial manufacture can be scientifically, sensitively and quickly monitored, meanwhile the problem of the shortage of reference substances is overcome.

The invention discloses an Apio americana medikus leaf flavone extract, a preparation method and applications thereof, wherein the Apio americana medikus leaf flavone extract contains 78-87% by mass of total flavonoids, the total flavonoids contain vitexin and schaftoside, and by using the Apio americana medikus leaf flavone extract as the reference, the mass fraction of the vitexin is 45-50%, and the mass fraction of the schaftoside is 19-24%. According to the present invention, by using the Apio americana medikus leaf as the object to extract the flavone substances, the obtained Apio americana medikus leaf flavone extract is rich in flavones, especially vitexin and schaftoside, can effectively inhibit the activity of alpha-glucosidase, can promote the carbohydrate metabolism of cells, and has blood glucose lowering activity.

The invention provides a method for extracting and separating novel Schaftoside. The method includes the steps of subjecting Desmodium styracifolium to reflux extraction via ethyl alcohol to obtain Desmodium styracifolium extract; subjecting the extract to multistage chromatographic separation via alcohol systems, to be specific, subjecting the extract to first chromatographic separation via the first alcohol system to obtain a first flow, subjecting the first flow to second chromatographic separation via the second alcohol system to obtain a second flow, and subjecting the second flow to third chromatographic separation via the third alcohol system to so as to obtain the novel Schaftoside. The process of separating and extracting the novel Schaftoside from Desmodium styracifolium is simple, production time is short, the yield is high, the purity is high, and the novel Schaftoside is up to above 95% in purity.

The invention relates to a quality detecting method for a traditional Chinese medicine extract, in particular to a quality detecting method for a desmodium styracifolium general flavone extract. The desmodium styracifolium general flavone extract is prepared through desmodium styracifolium smashing and alcohol or water extraction, ethyl alcohol recovery, concentration, macroporous adsorption resincolumn chromatography elution (ethyl alcohol-water system), concentration and vacuum drying. The preparation method of the desmodium styracifolium general flavone extract is reasonable, stable, shortin production period and suitable for industrial production. An ultraviolet-visible spectrophotometry method, a high efficiency liquid chromatography method and an area normalization method are adopted to determine the content of general flavone and schaftoside in the desmodium styracifolium general flavone extract, and the method is accurate, stable and simple in operation.

The invention discloses a quality detection method of Dendrobium loddigesii Rolfe. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium loddigesii Rolfe; and 4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the fingerprint model related to the chemical small molecule component. According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium loddigesii Rolfe is precisely identified and controlled.

The invention discloses a quality detection method for Dendrobium devonianum Paxton. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium devonianum Paxton herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / ornaringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium devonianum Paxton; and 4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium devonianum Paxton is precisely identified and controlled.

The invention discloses a quality detection method of Dendrobium denneanum Kerr. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the varietyof a to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium denneanum Kerr; and 4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. Accordingto the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium denneanum Kerr is precisely identified and controlled.

The invention discloses a quality detecting method of Dendrobium aphyllum. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of ato-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium aphyllum; and 4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint asthe independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium aphyllum is precisely identified and controlled.

The invention discloses a quality detection method of Dendrobium nobile Lindley. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the varietyof a to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium nobile Lindley; and 4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. Accordingto the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium nobile Lindley is precisely identified and controlled.

The invention belongs to the technical field of chemistry and medicine, and discloses an efficient total synthesis method and application of a natural product schaftoside. The method comprises the steps of subjecting naringenin, tert-butyldimethylsilyl chloride and acetyl chloride to reaction to obtain an intermediate compound 2, and conducting deoxidation reaction to obtain an intermediate compound 3; carrying out rearrangement reaction on the intermediate compound 3 to obtain an intermediate compound 5, and removing a silicon-based protecting group to obtain an intermediate compound 6; carrying out rearrangement reaction on the intermediate compound 6 to obtain an intermediate compound 8; reacting the intermediate compound 8 with benzyl bromide in potassium carbonate to obtain an intermediate compound 9; oxidizing the intermediate compound 9 by ceric ammonium nitrate and pyridinium dichromate to obtain an intermediate compound 10; removing benzyl from the intermediate compound 10 to obtain an intermediate compound 11; and catalyzing the intermediate compound 11 to obtain an intermediate compound 12, and removing acetyl to obtain a target product compound. The method is mild in reaction condition, convenient to operate, economical in raw materials and capable of being used for industrial preparation.

The invention discloses an infrared spectroscopy on-line quality monitoring and controlling system applied to anaesthetic preparations. The infrared spectroscopy on-line quality monitoring and controlling system comprises the following steps: A, a collector on a multi-functional extracting pot is used for collecting the infrared spectroscopy of a solution on line, sampling is carried out at the same time, and the content of chlorogenic acid and schaftoside is measured off line; B, a collector on a concentration pot is used for collecting the infrared spectroscopy of the solution on line, sampling is carried out at the same time, and the content of index components is measured off line; C, the infrared spectroscopy and content data obtained from the step A and the step B, of a sample is inputted into a computer to obtain the content of the index components so as to realize real-time monitoring of the index components; D, the content of the index components obtained from an on-line analysis mathematic model is inputted into a distributed control system through an OPC communication module, and an self-controlling valve field actuator in anaesthetic preparation equipment is controlled to realize the feedback control of the anaesthetic preparations in the preparing process. According to the invention, the content of the index components can be monitored in real time, and the feedback of anaesthetic composition in the preparing process can be realized.

The invention discloses a quality detection method of Dendrobium chrysotoxum Lindley. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as areference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium chrysotoxum Lindley; and4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other componentsin the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component.According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium chrysotoxum Lindley is precisely identified and controlled.

The invention provides a method for extracting and separating schaftoside from desmodium styracifolium. The method comprises the following steps that reflux extraction is performed on the desmodium styracifolium by utilizing ethyl alcohol to obtain a desmodium styracifolium extractive; multi-level chromatographic separation is performed on the extractive by utilizing alcohol systems, wherein first-level chromatographic separation is performed on the extractive by utilizing the first alcohol system to obtain a first separation part, second-level chromatographic separation is performed on the first separation part by utilizing the second alcohol system to obtain the schaftoside. The invention further provides a system for implementing the method. The schaftoside is separated and extracted from the desmodium styracifolium by utilizing the method and / or the system, the technology is easy to operate, the production time is short, the production yield is high, the purity is high, and the schaftoside with the purity more than 98 percent can be obtained.

The invention provides a flavonoid extract containing schaftoside, which is prepared from raw traditional Chinese medicinal herbal materials medicine raw materials. The invention also relates to the application of the flavonoid extract in treating cerebral ischemia. The invention particularly provides a flavonoid extract prepared from the raw medicinal material of rhizoma arisaematisarisaema tuber raw material medicine and an extraction method thereof. The invention also provides a medicinal composition adopting the extract. The extraction method is simple and practical, and the extraction effect is favorable. The medicinal composition has an obvious determined curative effect, which and has a brand new application in the aspect for treating cerebrovascular diseases.

The invention relates to the field of medicines, in particular to composition and an application of the composition to protection of HK-2 cells damaged by calcium oxalate monohydrate. The compositionis mainly prepared from following components in percentage by mass: 5%-11% of Vicenin-1, 8%-15% of Vicenin-2, 8%-15% of Vicenin-3, 15%-60% of Schaftoside and 23%-45% of Schaftoside. By means of cooperation of five compounds, the composition in the embodiment of the invention protects the HK-2 cells damaged by calcium oxalate monohydrate and further can be used for preparing medicines for treatingkidney stone. The composition can be combined with specific sites on the HK-2 cells, and is an effective component for playing the pharmaceutical effect.

The invention belongs to the technical field of phytochemistry. The invention discloses a method for simultaneously separating schaftoside, vichnine-2 and ecdysone and application. Elsholtzia rugulosais used as an extraction raw material, 90% ethanol is used as an extraction solvent, the extracting solution is concentrated under reduced pressure, and the extracting solution is extracted with petroleum ether, ethyl acetate and n-butyl alcohol in sequence; the concentrated n-butyl alcohol extraction part is coarsely separated by using AB-8 macroporous resin to obtain a 50% ethanol elution part;and ODS column chromatography separation is performed on the eluted part to obtain an ecdysone monomer, impurity-containing schaftoside and impurity-containing vichnine-2, and silica gel column chromatography separation and purification are respectively performed on the impurity-containing schaftoside and impurity-containing vichnine-2 to respectively obtain a schaftoside monomer and a vichnine-2monomer. The method is short in separation and purification process, simple to operate and easy to implement, and plant resources and extraction and separation cost are greatly saved; and great economic benefits can be generated, and the development of industries in related fields can be promoted.

The invention provides a calculus eliminating tablet fingerprint establishing method. The method comprises the following steps: preparing a reference substance, preparing a test sample, and using high performance liquid chromatography (HPLC) to obtain the fingerprint of a calculus eliminating tablet; wherein in the chromatography and system applicability tests, octadecyl silane bonded silica gel is taken as the filling agent, acetonitrile is taken as the mobile phase (A), a phosphoric acid water solution (0.2%) is taken as the mobile phase (B), methanol is taken as the mobile phase (C), the detection wavelength is 272, and the theoretical plate number calculated by schaftoside is not less than 3000.

The invention discloses a method for extracting schaftoside from herba desmodii styracifolii. The method comprises the following steps: (1) cleaning and air drying the herba desmodii styracifolii, pulverizing the herba desmodii styracifolii by using a pulverizing machine, and sieving; (2) subcritical water extracting: placing the processed raw material in an extraction kettle, adding hydroxyethylcellulose equivalent to 0.001 parts by weight of the raw material, injecting deoxygenated deionized water into the kettle, wherein the extraction temperature is 150 to 160 DEG C, the pressure is 8 to10Mpa, the extraction time is 0.5 to 1.0h, extracting the herba desmodii styracifolii, after the extraction is ended, releasing the pressure, enabling extract liquid to flow into a collection tank, cooling to room temperature, and obtaining the extract liquid; and (3) purifying: concentrating the extract liquid obtained in step (2), performing the column chromatography by virtue of macroporous resin, eluting by using 50 percent by volume of ethanol, obtaining eluent, concentrating, and drying, thus obtaining a finished product.

The invention discloses a quality detection method of Dendrobium fimbriatum Hook. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety of a to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium fimbriatum Hook; and 4) establishing an analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in thefingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. According to the present invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium fimbriatum Hook is precisely identified and controlled.

The invention discloses a method for establishing fingerprints of related characteristics of chemical small molecule components of noble dendrobium. The method comprises: 1) respectively performing chromatographic detection on samples having a sample size of n, and obtaining test data with chemical small molecule components schaftoside and / or naringenin as reference components; 2) respectively performing fingerprint analysis on the samples to obtain a peak area value of the fingerprints of all chemical components of noble dendrobium; 3) taking the component content value of schaftoside and / ornaringenin in chromatographic data as a response variable, taking a peak area value of other components in the fingerprint as an independent variable to establish an analytical model, and establishinga linear regression model of the small molecular components and the fingerprints; 4) characterizing the contribution degree of the independent variable to regression modeling through a variable correlation coefficient index, and establishing the fingerprints of the related characteristics of the chemical small molecule components. According to the method, correlation analysis of the chemical small molecule component content is achieved, and the quality of the noble dendrobium medicinal material can be effectively identified, controlled and evaluated.

The invention discloses a method for building a relevant characteristic fingerprint spectrum of dendrobium devonianum chemical micromolecular compositions, the method comprises the following steps that (1) samples with the sample sizes of n are subjected to chromatographic detection respectively, and the chemical micromolecular composition schaftoside and / or naringenin are / is taken as detection data of reference ingredients; (2) the samples are subjected to fingerprint spectrum detection, and the fingerprint spectrum peak area value of the whole chemical composition of dendrobium devonianum isobtained; (3) the composition content of the schaftoside and / or naringenin in the spectrum data are / is taken as response variables, the peak area values of other compositions in the fingerprint spectrum are taken as independent variables to build an analysis model, and a micromolecular composition and fingerprint spectrum linear regression model is built; (4) the relevant characteristic fingerprint spectrum of the micromolecular compositions is built for the contribution degree of the regression model through the variable correlation coefficient index representation independent variables. According to the method for building the relevant characteristic fingerprint spectrum of the dendrobium devonianum chemical micromolecular compositions, the correlation analysis of the chemical micromolecular composition contents is realized, and the quality of the dendrobium devonianum medical material can be effectively identified, controlled and evaluated.

The invention provides a method and a system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii. The compound monomers are schaftoside, luteolin-6-C-beta-D-glucopyranose-8-C-beta-xylopyranoside and Vicenin-2. The method comprises the following steps: carrying out reflux extraction on herba desmodii styracifolii by using ethanol to obtain herba desmodii styracifolii extract; and carrying out multi-stage chromatographic separation on the extract by using an alcohol system, wherein the step comprises: carrying out first chromatographic separation on the extract by using a first alcohol system to obtain a first separated part; carrying out second chromatographic separation on the first separated part by using a second alcohol system; and sequentially obtaining three compound monomers according to the sequence of detection peaks. The invention provides a system for implementing the method. According to the method and / or system for simultaneously extracting three c-glycosylflavone compound monomers from herba desmodii styracifolii provided by the invention, the process is simple to operate, the production time is short, and the product is high in yield and high in purity.

The invention provides rhizoma alismatis granules with the efficacies of removing and preventing urinary calculus used for preventing and treating urinary calculus. A preparation method of the rhizoma alismatis granules comprises the following steps: taking the five medicinal materials, including rhizoma alismatis, herba plantaginis, herba desmodii styracifolii, poria cocos and cassia twig as the raw materials, adding with 8-fold amount of water, decocting the medicinal materials for twice, wherein each time is 2h, mixing the decoctions, filtering the obtained mixed decoction, concentrating the filtrate to obtain extract, adding with 1.5-fold amount of dextrin and 0.004-fold amount of steviosin, carrying out uniform mixing by adopting 80% alcohol as a wetting agent (the use amount of alcohol is 10% of that of the extract), and preparing the granules by adopting a conventional method. A quality detection method is characterized in that a thin-layer chromatography of herba desmodii styracifolii and cassia twig in the medicinal materials is adopted as an identification method, specifically, the contents of two active ingredients, namely, plantamajoside and schaftoside in the medicinal materials are measured by adopting a high performance liquid chromatography. The product is used for preventing and treating urinary calculus, and an extraction process is simple, stable and feasible; a quality standard detection method of the rhizoma alismatis granules with the efficacies of removing and preventing urinary calculus is sensitive and reliable and is strong in specificity.

The invention discloses a construction method for an HPLC fingerprint of a bone-invigorating injection and a quality detection method of the bone-invigorating injection. The method takes schaftoside,vicenin-2 and vicenin-3 as reference substances, the chromatographic conditions are that acetonitrile is used as a mobile phase A, and a glacial acetic acid solution of which the concentration is 0.3%is used as a mobile phase B; the elution time is 0 to 7min, correspondingly, the mobile phase A is changed to 10-20% of A, and the mobile phase B is changed to 90 to 80%; the column temperature is 30DEG C; HPLC analysis is carried out under the circumstance that the detection wavelength is 340nm to construct the fingerprint. A common pattern fingerprint is constructed through ten batches or moreof bone-invigorating injection samples, and is compared with a sample to be tested to detect the quality of the bone-invigorating injection. The method disclosed by the invention can monitor the quality of a bone-invigorating injection finished or semi-finished product, and provide a scientific basis for standardization study of the bone-invigorating injection, and is further strong in repeatability, precision and specificity.