Method for aseptic seeding and tissue culture of anemone roxburghii

A tissue culture and aseptic sowing technology, applied in the fields of botany equipment and methods, horticultural methods, plant genetic improvement, etc., can solve the problems of low reproduction coefficient, difficulty in large-scale planting, and difficulty in germination, and achieve the effect of improving the proliferation rate.

Inactive Publication Date: 2019-01-04
翁源县天下泽雨农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for aseptic sowing and tissue culture of A. clematis, which solves the problem that the seeds of A. clematis are tiny and consist of immature embryos and several layers of seed coat cells, which are generally not easy to germinate. Propagation by split root method or cutting method, the reproduction coefficient is low, and it is difficult to plant on a large scale

Method used

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  • Method for aseptic seeding and tissue culture of anemone roxburghii
  • Method for aseptic seeding and tissue culture of anemone roxburghii
  • Method for aseptic seeding and tissue culture of anemone roxburghii

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Embodiment 1

[0025] like figure 1 As shown, the present invention provides a kind of technical scheme: a kind of aseptic seeding and tissue culture method of Clematis clematis, comprising the following steps:

[0026] a), Pollination of Aureus taiwanensis: In October, the flowering individuals of A. climatis taiwanensis with good growth are selected for pollination. During pollination, the pollen blocks of the female flowers (female parent) are removed (emasculated), and then the pollen of the collected male parent is removed. The block is gently placed on the stigma of the female parent with small tweezers, and the label is hung;

[0027] b) Sowing of clematis taiwanensis: select 8 mature capsules of clematis taiwanensis without cracking and wormholes, wash them with running water, then wipe the capsules with cotton pads dipped in 75% alcohol, then soak and shake in 10 times bleach water 10 minutes, then rinse 4 times with sterile water, dry the water, cut a small hole in the capsule wit...

Embodiment 2

[0033] A method for aseptic seeding and tissue culture of Clematis clematis, comprising the following steps:

[0034] a), Pollination of Aureus taiwanensis: In October, the flowering individuals of A. climatis taiwanensis with good growth are selected for pollination. During pollination, the pollen blocks of the female flowers (female parent) are removed (emasculated), and then the pollen of the collected male parent is removed. The block is gently placed on the stigma of the female parent with small tweezers, and the label is hung;

[0035]b) Sowing of clematis taiwanensis: select 8 mature capsules of clematis taiwanensis without cracking and wormholes, wash them with running water, then wipe the capsules with cotton pads dipped in 75% alcohol, then soak and shake in 10 times bleach water 13 minutes, then rinse with sterile water 4 times, dry the water, cut a small hole in the capsule with a sterile scalpel, and sprinkle the seeds in the capsule evenly on the medium: American...

Embodiment 3

[0043] A method for aseptic seeding and tissue culture of Clematis clematis, comprising the following steps:

[0044] a), Pollination of Aureus taiwanensis: In October, the flowering individuals of A. climatis taiwanensis with good growth are selected for pollination. During pollination, the pollen blocks of the female flowers (female parent) are removed (emasculated), and then the pollen of the collected male parent is removed. The block is gently placed on the stigma of the female parent with small tweezers, and the label is hung;

[0045] b) Sowing of clematis taiwanensis: select 9 mature capsules of clematis taiwanensis without cracking and wormholes, wash them with running water, then wipe the capsules with cotton pads dipped in 75% alcohol, then soak and shake in 10 times bleaching water 15 minutes, then rinse 4 times with sterile water, dry the water, cut a small hole in the capsule with a sterile scalpel, and sprinkle the seeds in the capsule evenly on the medium: Amer...

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Abstract

The invention discloses a method for aseptic seeding and tissue culture of anemone roxburghii, which comprises the following steps: a) pollination of Taiwan anemone roxburghii; b) seeding of the Taiwan anemone roxburghii: capsules are selected and rinsed with running water, absorbent cottons soaked in alcohol with concentration of 75% are used for wiping the capsules, the capsules are then soakedin bleaching water with concentration of 10% f and shaken or 15 minutes, aseptic water is used for washing the capsules for 3-4 times, the capsules are aired under the sun for drying the water thereon, an aseptic scalpel is used for cutting a small opening on each capsule, and seeds picked from the capsules are evenly spread in a culture medium; c) stem proliferation of the Taiwan anemone roxburghii: stocky Taiwan anemone roxburghii seedlings with reddish leaves are treated by removing the leaves, stems with only two stem nodes are laid and inoculated in the culture medium with light pressure;and d) rooting culture of the Taiwan anemone roxburghii: the seedlings shorter than 2 cm and taller than 3 cm are separately inoculated in the culture medium. The method utilizes the sexual multiplication of the seeds, thereby facilitating the gene exchange, enabling the offspring individuals to be rich in vitality and more adaptive to new environments, and providing strong support for preservation of germplasm resources and continuation of species.

Description

technical field [0001] The invention relates to the technical field of clematis cultivation, in particular to a method for aseptic sowing and tissue culture of clematis. Background technique [0002] Known as "golden grass", "miracle medicine" and "bird ginseng", Taiwan golden clematis is a plant of the genus Orchidaceae. It has the functions of viewing leaves and flowers, and is a treasure of indoor flowers with high ornamental value. Its whole herbal medicine is sweet, light, flat, and cool in nature and flavor. It has the effects of clearing heat, cooling blood, dispelling wind and dampness, strengthening the heart and diuresis, strengthening the kidney and calming the liver, and lowering blood pressure. Snake bites all have significant curative effects, and are known as the "king of medicines". The remarkable efficacy and health care functions of Taiwan clematis have been recognized by the world, and its products have broad market prospects. [0003] The germination rat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02
CPCA01H1/02A01H4/008
Inventor 陈绪盛杜正丽毛明辉
Owner 翁源县天下泽雨农业科技有限公司
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