Method for aseptic seeding and tissue culture of anemone roxburghii
A tissue culture and aseptic sowing technology, applied in the fields of botany equipment and methods, horticultural methods, plant genetic improvement, etc., can solve the problems of low reproduction coefficient, difficulty in large-scale planting, and difficulty in germination, and achieve the effect of improving the proliferation rate.
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Embodiment 1
[0025] like figure 1 As shown, the present invention provides a kind of technical scheme: a kind of aseptic seeding and tissue culture method of Clematis clematis, comprising the following steps:
[0026] a), Pollination of Aureus taiwanensis: In October, the flowering individuals of A. climatis taiwanensis with good growth are selected for pollination. During pollination, the pollen blocks of the female flowers (female parent) are removed (emasculated), and then the pollen of the collected male parent is removed. The block is gently placed on the stigma of the female parent with small tweezers, and the label is hung;
[0027] b) Sowing of clematis taiwanensis: select 8 mature capsules of clematis taiwanensis without cracking and wormholes, wash them with running water, then wipe the capsules with cotton pads dipped in 75% alcohol, then soak and shake in 10 times bleach water 10 minutes, then rinse 4 times with sterile water, dry the water, cut a small hole in the capsule wit...
Embodiment 2
[0033] A method for aseptic seeding and tissue culture of Clematis clematis, comprising the following steps:
[0034] a), Pollination of Aureus taiwanensis: In October, the flowering individuals of A. climatis taiwanensis with good growth are selected for pollination. During pollination, the pollen blocks of the female flowers (female parent) are removed (emasculated), and then the pollen of the collected male parent is removed. The block is gently placed on the stigma of the female parent with small tweezers, and the label is hung;
[0035]b) Sowing of clematis taiwanensis: select 8 mature capsules of clematis taiwanensis without cracking and wormholes, wash them with running water, then wipe the capsules with cotton pads dipped in 75% alcohol, then soak and shake in 10 times bleach water 13 minutes, then rinse with sterile water 4 times, dry the water, cut a small hole in the capsule with a sterile scalpel, and sprinkle the seeds in the capsule evenly on the medium: American...
Embodiment 3
[0043] A method for aseptic seeding and tissue culture of Clematis clematis, comprising the following steps:
[0044] a), Pollination of Aureus taiwanensis: In October, the flowering individuals of A. climatis taiwanensis with good growth are selected for pollination. During pollination, the pollen blocks of the female flowers (female parent) are removed (emasculated), and then the pollen of the collected male parent is removed. The block is gently placed on the stigma of the female parent with small tweezers, and the label is hung;
[0045] b) Sowing of clematis taiwanensis: select 9 mature capsules of clematis taiwanensis without cracking and wormholes, wash them with running water, then wipe the capsules with cotton pads dipped in 75% alcohol, then soak and shake in 10 times bleaching water 15 minutes, then rinse 4 times with sterile water, dry the water, cut a small hole in the capsule with a sterile scalpel, and sprinkle the seeds in the capsule evenly on the medium: Amer...
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