Method for tissue culture of hoya kerrii
A technique for tissue culture and Corydalis chinensis, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inability to alleviate Cortica vulgaris, cannot meet industrial production, low reproduction coefficient, etc., and achieves improved reproduction. Coefficient and reproduction speed, ease of transplanting and planting management, cost reduction effect
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Embodiment 1
[0063] (1) Disinfection and sterilization
[0064] The wild Orchid cordifolia was collected from Laos, and the plants with better growth potential and higher ornamental value of leaves were selected as female parents. Pick the leaves from the mother plant, wash them in running water with washing powder for 15 minutes; soak the cleaned leaves in 70% alcohol for 30 seconds in an ultra-clean workbench, and rinse them with sterile water; then immerse the leaves in 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water 4 times.
[0065] (2) Callus induction culture
[0066] Put the surface-sterilized material on the sterilized filter paper and cut it into 0.5cm with a scalpel 2 The small pieces were inoculated on the induction medium, and the medium components were: MS basic medium, 3% glucose, 0.7% agar, 6-benzylpurine (6-BA) 1.0mg / L, naphthaleneacetic acid (NAA ) 0.8mg / L, 2,4 dichlorophenoxyacetic acid (2-4D) 0.8mg / L.
[0067] The material was cultivated at...
Embodiment 2
[0081] (1) Disinfection and sterilization
[0082] The wild Orchid cordifolia was collected from Laos, and the plants with better growth potential and higher ornamental value of leaves were selected as female parents. Pick the leaves from the mother plant, wash them in running water with washing powder for 25 minutes; soak the cleaned leaves in 75% alcohol for 30 seconds in an ultra-clean workbench, and rinse them with sterile water; then immerse the leaves in 0.1% mercuric chloride solution for 15 minutes, rinsed with sterile water 5 times.
[0083] (2) Callus induction culture
[0084] The surface sterilized material was cut into 0.8 cm square pieces with a scalpel on the sterilized filter paper, and inoculated on the induction medium. The medium components were: MS basic medium, 3% glucose, 0.7% agar, 6-benzylpurine (6-BA) 1.5mg / L, naphthaleneacetic acid (NAA) 1.0mg / L, 2,4 dichlorophenoxyacetic acid (2-4D) 1.0mg / L.
[0085] The material was cultivated at a temperature of...
Embodiment 3
[0099] (1) Disinfection and sterilization
[0100]The wild Orchid cordifolia was collected from Laos, and the plants with better growth potential and higher ornamental value of leaves were selected as female parents. Pick the leaves from the mother plant, wash them in running water with washing powder for 30 minutes; soak the cleaned leaves in 75% alcohol for 30 seconds in an ultra-clean workbench, and rinse them with sterile water; then immerse the leaves in 0.1% mercuric chloride solution for 20 minutes, rinsed with sterile water 5 times.
[0101] (2) Callus induction culture
[0102] Cut the surface-sterilized material on sterilized filter paper into small pieces of 1 cm square with a scalpel, and inoculate it on the induction medium. The composition of the medium is: MS basic medium, 3% glucose, 0.7 % agar, 6-benzylpurine (6-BA) 2.0mg / L, naphthaleneacetic acid (NAA) 1.2mg / L, 2,4 dichlorophenoxyacetic acid (2-4D) 1.2mg / L.
[0103] The material was cultivated at a tempera...
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