Method for tissue culture of hoya kerrii

A technique for tissue culture and Corydalis chinensis, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inability to alleviate Cortica vulgaris, cannot meet industrial production, low reproduction coefficient, etc., and achieves improved reproduction. Coefficient and reproduction speed, ease of transplanting and planting management, cost reduction effect

Inactive Publication Date: 2012-07-18
云南山里红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some literatures have reported on the tissue culture of Cordella cordifolia, its reproduction coefficient is low and the cost is high, which is far from meeting the needs of industrial production, and can not alleviate the lack of situation in the market.

Method used

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  • Method for tissue culture of hoya kerrii
  • Method for tissue culture of hoya kerrii
  • Method for tissue culture of hoya kerrii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] (1) Disinfection and sterilization

[0064] The wild Orchid cordifolia was collected from Laos, and the plants with better growth potential and higher ornamental value of leaves were selected as female parents. Pick the leaves from the mother plant, wash them in running water with washing powder for 15 minutes; soak the cleaned leaves in 70% alcohol for 30 seconds in an ultra-clean workbench, and rinse them with sterile water; then immerse the leaves in 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water 4 times.

[0065] (2) Callus induction culture

[0066] Put the surface-sterilized material on the sterilized filter paper and cut it into 0.5cm with a scalpel 2 The small pieces were inoculated on the induction medium, and the medium components were: MS basic medium, 3% glucose, 0.7% agar, 6-benzylpurine (6-BA) 1.0mg / L, naphthaleneacetic acid (NAA ) 0.8mg / L, 2,4 dichlorophenoxyacetic acid (2-4D) 0.8mg / L.

[0067] The material was cultivated at...

Embodiment 2

[0081] (1) Disinfection and sterilization

[0082] The wild Orchid cordifolia was collected from Laos, and the plants with better growth potential and higher ornamental value of leaves were selected as female parents. Pick the leaves from the mother plant, wash them in running water with washing powder for 25 minutes; soak the cleaned leaves in 75% alcohol for 30 seconds in an ultra-clean workbench, and rinse them with sterile water; then immerse the leaves in 0.1% mercuric chloride solution for 15 minutes, rinsed with sterile water 5 times.

[0083] (2) Callus induction culture

[0084] The surface sterilized material was cut into 0.8 cm square pieces with a scalpel on the sterilized filter paper, and inoculated on the induction medium. The medium components were: MS basic medium, 3% glucose, 0.7% agar, 6-benzylpurine (6-BA) 1.5mg / L, naphthaleneacetic acid (NAA) 1.0mg / L, 2,4 dichlorophenoxyacetic acid (2-4D) 1.0mg / L.

[0085] The material was cultivated at a temperature of...

Embodiment 3

[0099] (1) Disinfection and sterilization

[0100]The wild Orchid cordifolia was collected from Laos, and the plants with better growth potential and higher ornamental value of leaves were selected as female parents. Pick the leaves from the mother plant, wash them in running water with washing powder for 30 minutes; soak the cleaned leaves in 75% alcohol for 30 seconds in an ultra-clean workbench, and rinse them with sterile water; then immerse the leaves in 0.1% mercuric chloride solution for 20 minutes, rinsed with sterile water 5 times.

[0101] (2) Callus induction culture

[0102] Cut the surface-sterilized material on sterilized filter paper into small pieces of 1 cm square with a scalpel, and inoculate it on the induction medium. The composition of the medium is: MS basic medium, 3% glucose, 0.7 % agar, 6-benzylpurine (6-BA) 2.0mg / L, naphthaleneacetic acid (NAA) 1.2mg / L, 2,4 dichlorophenoxyacetic acid (2-4D) 1.2mg / L.

[0103] The material was cultivated at a tempera...

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Abstract

The invention relates to a method for tissue culture of a plant, in particular to a method for tissue culture of hoya kerrii, which belongs to the technical field of agricultural cultivation and includes the steps of sterilization, induced culture of calluses, enrichment culture, bud differentiation culture, seedling strengthening and bud propagation culture and rooting culture. By the aid of the tissue culture technology, when the hoya kerrii is propagated by means of plant tissue culture, the rapid propagation advantage is exerted, and a lot of seedlings can be obtained in a short period; and the plant tissue culture serves as a vegetative propagation method, seedlings are uniform, so that later uniform transplantation and planting management are facilitated, and the consistencies of various characters of grown commercial adult plants and potted plants are also guaranteed. Compared with former propagation methods, according to ingredients of the culture medium and the matched culture methods involved in the method for tissue culture of hoya kerrii, cost can be reduced, the propagation coefficient and the propagation speed can be increased, and the method is applicable to large-scale industrial production.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Cordella cordifolia, belonging to the technical field of agricultural planting. Background technique [0002] Heart leaf ball orchid is a plant of the genus Ball orchid of the Milkweed subfamily of Romoaceae, also known as concave leaf ball orchid, wax orchid and wax flower, etc., native to Thailand, Laos and other places. It is an evergreen woody vine, which can grow by climbing on other things. The vines can be more than 5 meters long and have aerial roots; the leaves are opposite, thick, heart-shaped, green; umbels, corollas are light green and fragrant. [0003] As a new type of ornamental plant that has been introduced in recent years, Cordula radiata has the characteristics of slow growth, drought tolerance, shade tolerance, and is suitable for indoor placement. It is a plant with great market development prospects. Heart leaf ball orchid is very ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 岳健张志敏李江黎李建李昆华
Owner 云南山里红生物科技有限公司
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