A kind of tissue culture method of linden seedlings

A technology for tissue culture and seedlings, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of no tissue culture methods for Tilia montina seedlings, and achieve the purpose of avoiding the spread of diseases and insect pests, shortening the breeding cycle, and improving the breeding quality. The effect of the coefficient

Active Publication Date: 2021-01-05
INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no corresponding tissue culture method for Tilia mongolica seedlings

Method used

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  • A kind of tissue culture method of linden seedlings
  • A kind of tissue culture method of linden seedlings
  • A kind of tissue culture method of linden seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0056] (1) Mix the linden seeds with fine sand in a weight ratio of 1:3, and rub gently until the surface of the seeds is rough. Separate the seeds and fine sand, wash the seeds with clean water, put them in a cup, and soak them in distilled water for 3 days.

[0057] (2) Filter and dry the seeds soaked in step (1), add concentrated sulfuric acid at a volume ratio of 1:1 between seeds and concentrated sulfuric acid, and soak for 3 minutes, stirring continuously during this period. After pouring out the sulfuric acid, carefully rinse with clean water until there is no residue, and then soak in sterile water overnight. Repeat the above steps 1 time.

[0058] (3) In the ultra-clean workbench, first soak the seeds treated in step (2) with an alcohol solution with a volume fraction of 75% for 40 seconds, then wash with sterile water for 3 to 5 times, and then use a solution with a mass concentration of 2 % sodium hypochlorite solution for 10 minutes, and then rinsed with sterile ...

Embodiment 1-2 to Embodiment 1-5

[0064] Change the type and amount of reagents in the subculture medium in step (3), and observe the effects of different hormone combinations on the reproductive coefficient of adventitious buds of Tilia montana. The dosage of sucrose and agar, and the cultivation method are the same as those in Example 1-1, and the results are shown in Table 1. Wherein, the multiplication factor=the total number of proliferation of explant growth points / the total number of differentiated explants, which is calculated by the method of calculating the average value.

[0065] Table 1

[0066]

[0067]

[0068] As can be seen from Table 1, compared with embodiment 1-1, change 6-BA, IBA and GA 3 The average plant height and multiplication factor of Tilia mongolica seedlings decreased. Therefore, for the subculture step, the most suitable subculture medium formula is WPM+3.0mg / L of 6-BA+1.0mg / L of IBA+3.0mg / L of GA 3 .

Embodiment 1-6 to Embodiment 1-8

[0070] Change the light conditions during the induction culture in step (2), and other culture methods are the same as in Example 1-1. See Table 2 for the effects of observing the germination rate, average plant height and growth of Tilia mongolica.

[0071] Table 2

[0072]

[0073] As can be seen from Table 2, dark culture and weak light culture are carried out successively in the induction process, and the germination rate of Tilia mongolica seeds is high, and the growth is robust; simple dark culture can easily cause seedlings to grow excessively and yellow, and are easy to die in the later stage; only low light is used The germination rate of cultured seeds is reduced; and strong light culture is not conducive to the growth of seedlings, making the seedlings short and deformed.

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Abstract

The invention provides a tissue culture method of tilia mongolica seedlings. The method comprises the following steps of (1) taking tilia mongolica seeds for sterilization treatment; (2) removing seedcoats of the sterilized tilia mongolica seeds and inoculating the seeds into an induction culture medium for induction culture to obtain adventitious buds; (3) inoculating the adventitious buds intoa subculture medium for subculture to obtain subculture seedlings; (4) inoculating the subculture seedlings into a rooting culture medium for rooting culture to obtain rooting seedlings. According tothe method, proliferation is carried out in the proliferation period at the speed rate of 3.8 times, and the rooting rate is 86%. The method can achieve culture of massive regenerated tilia mongolicaseedlings at a time, solve the problems of seed dormancy and slow growth of seedlings, and avoid the spread of plant diseases and insect pests caused by a cutting mode. The method can obviously increase the breeding coefficient of tilia mongolica and shorten the breeding cycle.

Description

technical field [0001] The invention relates to the technical field of tree breeding, in particular to a method for tissue culture of linden seedlings. Background technique [0002] Tilia mongolica is a positive tree that grows in mountain areas. It is the main companion tree species in birch poplar forests or mountain miscellaneous forests in summer green broad-leaved forests. It can become a dominant species in some areas and form small linden forests. There are also scattered growths in the mountains of the steppe belt. This tree species is widely distributed in Mongolia and Shanxi, Hebei, Liaoning, Inner Mongolia, Henan and other places in mainland China, and has been introduced and cultivated artificially. Tilia wood is light and soft, and can be used for furniture and cooking utensils; the flowers can be used as medicine, the seeds can be pressed for oil for industrial use, and the bark fibers can be used to make ropes. Tilia mongolica is an ideal ecological and econ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵泉胜铁英白玉娥彭鹏周晓炜冯建芳
Owner INNER MONGOLIA HESHENG ECOLOGICAL SILVICULTURE CO LTD
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