Phalaenopsis sterile root propagation method

A technology of Phalaenopsis and roots, which is applied in the field of red flower Phalaenopsis mycoroots to induce clustered buds to proliferate and cultivate into seedlings. It can solve the problems of poor ornamental performance of native species, shorten the breeding cycle, stabilize growth traits, and increase the breeding coefficient. Effect

Inactive Publication Date: 2014-10-01
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are about 70 kinds of native species in the world, and there are more than 530 varieties through hybridization, but the ornamental value of native species is poor, and most of the varieties used in commercial mass production are hybrids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] The present invention comprises the following technical steps:

[0014] (1) Select the plants of the Phalaenopsis safflower variety without diseases and insect pests, and cut the 2-3cm pedicel stems with axillary buds as explant materials; remove the outer bracts of the bud points with tweezers, and add 2 drops of Tween 20 Rinse under tap water for half an hour, transfer to the ultra-clean workbench and sterilize with 75% alcohol for 30 seconds. Finally, pour out the alcohol, treat it twice with sterile water, add a 2% NacloO solution for 5-6 minutes, pour out the NacloO, rinse it with sterile water for 4-5 times, and dry the surface with sterile filter paper; Remove the surface of the upper and lower ends of the pedicel that is in contact with the disinfectant, and inoculate it into the primary medium for culture. The primary culture of pedicels is: 1 / 2MS + 5mg / L 6-BA + 0.5mg / L NAA + 30g / L, sucrose + 6 g / L agar + 400mg / L, citric acid + 40g / L banana puree, the pH is 5...

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PUM

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Abstract

The invention provides a phalaenopsis sterile root propagation method, belongs to the technical field of seedlings of plant cultivation, and particularly relates to a method for cultivating seedlings of red phalaenopsis flower which is prepared by proliferating cluster buds induced by phalaenopsis sterile roots. According to the invention, plants of excellent pest-free phalaenopsis are selected; flower peduncle axillary bud stems in length of 2 cm are cut off from the robust mother plants to be used as an explant material; after being subjected to one month of material primary generation induction cultivation, grown-out axillary buds of the explant material are cut off and subjected to 40-day rooting cultivation; roots are cut off from the axillary buds, and are inoculated to a cluster bud induction culture medium; a plurality of buds can be grown out from the cutting points after 20 days, the cultivation is continued, and a large number of cluster buds can be grown out; the cluster buds can be cut off for rooting cultivation after being grown up, and a large number of cluster buds can be obtained again by continuously cultivating the sterile roots. The method has the advantages that a large number of bluster buds can be obtained by proliferation of the cluster buds from induced sterile roots to realize propagation of the phalaenopsis, so that the industrialized production of the phalaenopsis is realized; by the adoption of the method provided by the invention, the breeding coefficient is greatly improved, the breeding period is shortened, and the survival rate can reach more than 90%.

Description

technical field [0001] The invention belongs to the seedling technology in the field of plant cultivation, and specifically relates to a method for cultivating seedlings by inducing clustered buds to proliferate with red Phalaenopsis aseptic roots. Background technique [0002] Safflower Phalaenopsis, also known as Phalaenopsis, belongs to tropical aerial orchids, and is a perennial herbaceous plant in the Orchidaceae Phalaenopsis. The origin is in Assam, Myanmar, Philippines, Taiwan and other Asian tropical regions. Its reproduction is slow, the growth cycle is long, the plant type is beautiful, the flowers are large and colorful, the flower shape is unique, and the flowering period is long-lasting. It is deeply loved by people from all over the world. One of the four most valuable ornamental tropical orchids. There are about 70 kinds of native species in the world, and there are more than 530 varieties through hybridization, but the ornamental value of native species i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 白玉娥何炎红武欣慧
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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