Phalaenopsis sterile root propagation method
A technology of Phalaenopsis and roots, which is applied in the field of red flower Phalaenopsis mycoroots to induce clustered buds to proliferate and cultivate into seedlings. It can solve the problems of poor ornamental performance of native species, shorten the breeding cycle, stabilize growth traits, and increase the breeding coefficient. Effect
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[0013] The present invention comprises the following technical steps:
[0014] (1) Select the plants of the Phalaenopsis safflower variety without diseases and insect pests, and cut the 2-3cm pedicel stems with axillary buds as explant materials; remove the outer bracts of the bud points with tweezers, and add 2 drops of Tween 20 Rinse under tap water for half an hour, transfer to the ultra-clean workbench and sterilize with 75% alcohol for 30 seconds. Finally, pour out the alcohol, treat it twice with sterile water, add a 2% NacloO solution for 5-6 minutes, pour out the NacloO, rinse it with sterile water for 4-5 times, and dry the surface with sterile filter paper; Remove the surface of the upper and lower ends of the pedicel that is in contact with the disinfectant, and inoculate it into the primary medium for culture. The primary culture of pedicels is: 1 / 2MS + 5mg / L 6-BA + 0.5mg / L NAA + 30g / L, sucrose + 6 g / L agar + 400mg / L, citric acid + 40g / L banana puree, the pH is 5...
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