The invention relates to a smearing agent for promoting poplar-willow-trunk wound healing. In every 1,000 parts by weight of the smearing agent, the smearing agent is prepared from, by weight, 0.002-5parts of a plant growth regulator, 11-125 parts of a high-molecular film forming agent, 0.1-10 parts of a tackifier, 1-50 parts of an inert carrier, 0.1-30 parts of an antimicrobial substance, 0.1-1part of a coloring indicator and the balance water; the high-molecular film forming agent is prepared from, by weight, 1-20 parts of polyvinyl alcohol, 5-50 parts of sodium carboxymethylcellulose, 5-50 parts of chitosan and 0-5 parts of polyethylene glycol. The smearing agent is nontoxic and harmless to poplar-willow cambial cells, and the wound healing speed of poplar-willow trunks can be increased. The invention also discloses a preparing method for the smearing agent for promoting poplar-willow-trunk wound healing, and the smearing agent prepared with the method is applied to poplar-willow-trunk wound healing treatment, and has the advantages of being wide in bactericidal spectrum, low in toxicity, free of residue, safe, reliable, capable of protect wounds and the like.