Nontoxic fast ginger seedling growing method
A kind of ginger and fast technology is applied in the field of non-toxic and fast seedling raising of ginger, which can solve the problems of long time occupation of land in the field, weak seedlings, and large usage of ginger, so as to shorten the time of seedling raising, reduce the occurrence of diseases, and provide economic benefits. Effect
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example 1
[0026] 1. Ginger Germination
[0027] Choose ginger with a full water content of 25-35% and no pests and diseases. Under the condition of darkness and 25°C, bury it in sandy soil with a water content of 30-33%, and cover it with grass and seal it for 1-2 days. When it reaches 0.5-1cm;
[0028] 2. Obtaining test-tube seedlings
[0029] (1) Induction of callus
[0030] ① Optimum selection of culture medium for inducing callus. Sterilize the sprouts obtained by germination with 75% alcohol for 30 seconds, rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride for 5 minutes, rinse with sterile water for 3 times, and rinse with sterile water for 30 seconds. Blot dry on filter paper; under aseptic conditions, cut shoot apical meristem tissue of 0.2-0.3 mm, place in different concentrations of BA, NAA medium, measure the growth of callus, and determine the best medium components; results As shown in the table below:
[0031]
[0032] ② Induce callus f...
example 2
[0047] After determining the best induced callus culture medium, cluster buds to obtain culture medium and rooting culture medium according to example 1, cultivate virus-free shoots;
[0048] (1) Ginger germination
[0049] Choose ginger with a full water content of 25-35% and no pests and diseases. Under the condition of darkness and 25°C, bury it in sandy soil with a water content of 33%, cover it with grass and seal it for 2 days, and the ginger buds will grow to 0.5-1cm when;
[0050] (2) Acquisition of test-tube seedlings
[0051] ①Sterilize the buds obtained from accelerated germination with 75% alcohol for 30 seconds, rinse them with sterile water for 3 times, then disinfect them with 0.1% mercuric acid for 5 minutes, rinse them with sterile water for three times, and dry them with filter paper; Under the condition of 0.3mm shoot apical meristem, cultured at 25℃, light 1500-3000Lux, 12 hours / day light conditions, the composition of the medium is: MS+1.2mg / LNAA+4.0mg / L...
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