Tissue culture regeneration method of Denmark hibiscus

A technique of tissue culture and hibiscus, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., to achieve high regeneration efficiency, avoid domestication and transplanting, avoid rooting in bottles and later domestication and transplanting effects

Active Publication Date: 2021-06-11
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on the breeding of Danish hibiscus using plant tissue culture technology is still blank.

Method used

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  • Tissue culture regeneration method of Denmark hibiscus
  • Tissue culture regeneration method of Denmark hibiscus
  • Tissue culture regeneration method of Denmark hibiscus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of tissue culture regeneration method of Danish hibiscus, concrete operation is as follows:

[0042](1) Select an excellent strain of Danish hibiscus that grows well, and cut the unlignified branches of the current year as explants; after removing the leaves, cut them into 7-10cm-sized sections, pack them in 1000ml beakers, and put them in running water Rinse for 5 minutes, add 2ml of hand sanitizer once to rinse; then wipe the stem with 75% absolute ethanol; then place the stem in an aseptic operating table, rinse with sterile water for 4 times, and then successively wash with 75% anhydrous ethanol Disinfect the surface with water and ethanol for 30 seconds, disinfect with 0.1% (w / v) mercuric acid solution for 2 minutes, and finally rinse with sterile water for 6 times; blot the surface moisture of the stem segment, and cut it into a stem segment with an axillary bud for later use.

[0043] (2) Plant the cut stems in step (1) vertically in the adventitious bud i...

Embodiment 2

[0047] A kind of tissue culture regeneration method of Danish hibiscus, concrete operation is as follows:

[0048] (1) Select an excellent strain of Danish hibiscus that grows well, cut out the semi-lignified branches of the year as explants; cut into 8cm-sized sections after removing leaves, pack them in 1000ml beakers, and rinse under running water 10min, add 0.5-1ml hand sanitizer twice to rinse; then wipe the stem with 75% absolute ethanol; Disinfect the surface with water and ethanol for 40 seconds, disinfect with 0.1% (w / v) mercuric acid solution for 3 minutes, and finally rinse with sterile water 8 times; blot the surface moisture of the stem segment, and cut it into a stem segment with an axillary bud for later use.

[0049] (2) if figure 1 As shown, the stem segments in step (1) are vertically planted in the adventitious bud induction medium, and the temperature is 25±2°C, the light intensity is 3000lx, and the light time is 14h in a constant temperature culture room...

Embodiment 3

[0053] A kind of tissue culture regeneration method of Danish hibiscus, concrete operation is as follows:

[0054] (1) Select an excellent strain of Danish hibiscus that grows well, cut out the lignified branches of the year as explants; cut into 10cm-sized sections after removing the leaves, put them in 1000ml beakers, and rinse them under running water for 10min , adding 3 times of 0.5ml hand sanitizer to rinse; then wipe the stems with 75% absolute ethanol; then place the stems in an aseptic operating table, rinse them with sterile water for 6 times, and then successively wash them with 75% absolute ethanol Disinfect the surface for 45 seconds, disinfect with 0.1% (w / v) mercuric chloride solution for 5 minutes, and finally rinse 8 times with sterile water; blot the surface moisture of the stem section, and cut it into a stem section with an axillary bud for later use.

[0055] (2) Plant the cut stems in step (1) vertically in the adventitious bud induction medium, and carry...

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PUM

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Abstract

The invention discloses a tissue culture regeneration method of Denmark hibiscus, and relates to the technical field of the Denmark hibiscus. The tissue culture regeneration method comprises the following steps of taking a current-year tender stem segment with axillary buds of an excellent strain of the Denmark hibiscus as an explant, carrying out surface disinfection, then carrying out adventitious bud induction and multiplication culture, and when adventitious buds extend to 2-3cm and have 2-4 complete leaves, carrying out in-bottle or out-bottle adventitious root induction to finally obtain a complete Denmark hibiscus regenerated plant. The tissue culture regeneration method disclosed by the invention has the beneficial effects that the tissue culture regeneration method is of great significance to large-scale propagation and variety improvement research of the excellent strain of the Denmark hibiscus.

Description

technical field [0001] The invention relates to the technical field of Danish hibiscus, in particular to a tissue culture regeneration method of Danish hibiscus. Background technique [0002] Danish hibiscus is a perennial plant of the Malvaceae Hibiscus genus. As a new improved variety of hibiscus, it not only has dark green leaves, rich flower colors, and a long flowering period (annual flowering can be achieved as long as sufficient nutritional conditions are ensured after flowering); it also has strong adaptability , can be cultivated indoors and outdoors, and has high ornamental value and economic value. [0003] In recent years, with the improvement of people's living standards and the increasing market demand for Danish hibiscus, the industrialized cultivation of Danish hibiscus is facing unprecedented opportunities. However, the lack of efficient and high-quality seedling breeding technology has become a bottleneck restricting its industrial development. [0004] T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 侯金艳吴丽芳王萍王大成苏鹏飞丁双双
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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