Tobacco pollen lyophilization and low-temperature mid-long-term preservation method
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A freeze-vacuum drying and preservation method technology, which is applied in the preservation of plants, botanical equipment and methods, applications, etc., can solve problems such as affecting pollen vitality, and achieve the effects of saving equipment investment, thorough dehydration, and strong vitality
Inactive Publication Date: 2013-07-31
TOBACCO RES INST OF HUBEI PROVINCE
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Problems solved by technology
To apply freeze-vacuum drying technology to the storage of tobacco pollen, the pre-freezing method and freeze-drying method of pollen must be solved first, followed by the pollen storage method after freezing and the pollen vitality recovery method after freezing and storage, and the average cost of each step It will affect the vitality of pollen. At present, there is no complete technical solution to realize the vacuum technology of freezing tobacco pollen.
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example 1
[0025] Example 1: On July 19, 2011, China Tobacco Enshi Breeding Base, took 20g of B37 pollen collected on that day, put it into four 125ml white wide-mouth bottles, each bottle was 5g, numbered I, II, III, IV respectively , to conduct a comparative test of different storage methods.
[0026] 1. The storage method is as follows:
[0027] The jar mouth that pollen numbering is I is housed is directly placed in the fresh-keeping storage (YC / T 367-2010 method) of refrigerator after adopting paraffin sealing;
[0028] Put the jar containing the pollen number II into a desiccator with a desiccant (silica gel), apply Vaseline on the mouth of the desiccator and seal it, then put the desiccator in a freezer at -18°C and store it in a low-temperature freezer method for storage;
[0029]The wide-mouth bottle with pollen No. III is preserved by freeze-drying and low-temperature medium- and long-term storage method described in the present invention. ), put the jar with fresh pollen ca...
example 2
[0061] Example 2: In 2012, at the China Tobacco Enshi Breeding Breeding Base, the pollen of B37, KY14, TN90, and KY16 collected in different periods were stored in the freeze-vacuum drying and low-temperature medium- and long-term preservation methods described in this method, and the pollen viability was respectively carried out. Detection and fruit setting rate survey, methods and results are as follows:
[0062] 1. Freeze vacuum drying, cryopreservation method, pollen viability detection method, pollen rehydration method, field pollination and fruit setting rate investigation method are the same as Example 1.
[0063] Table 6 Comparison of pollen moisture results after different collection periods of different varieties
[0064]
[0065] 2. Pollen viability test results
[0066] As of April 2013, the test results of pollen viability stored for 8 months are shown in Table 7. The pollen viability of different varieties is above 80%. Compared with before treatment, the via...
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Abstract
The invention provides a tobacco pollen lyophilization and low-temperature mid-long-term preservation method. The tobacco pollen preservation method comprises the specific steps that: fresh pollen is placed into a pollen storage container; the container is covered and is placed in a cold trap of a lyophilization machine; the pollen is pre-frozen for 2-4h under a temperature of -45 DEG C to -50 DEG C; the pollen is fetched and is paved on a drying plate or a material box of the lyophilization machine; the pollen is lyophilized for 8-12h in the lyophilization machine under a temperature of -40 DEG C to -50 DEG C; the pollen is sealed in a pollen storage bottle or pollen storage bag; the pollen is placed in a freezer under a temperature of -4 DEG C to -18 DEG C, and can be preserved for a long time in low temperature till a pollination period; the pollen is fetched, the sealing is removed, and pollen activity is recovered under room temperature. With the method provided by the invention, a problem of flowering period missing during a tobacco hybrid seed production process is solved. The male parent is planted in advance, and the plantation of female parent is arranged according to the male parent collection amount. Therefore, blind plantation of parents and soil resource waste are reduced, pollination rate is greatly improved, and cost is greatly reduced.
Description
technical field [0001] The invention relates to a method for storing agricultural crop pollen, in particular to a method for freeze-drying, low-temperature, medium- and long-term storage of pollen suitable for crops such as tobacco. Background technique [0002] In the process of tobacco male sterile hybrid first-generation seed production, effectively overcoming the failure of the parents to meet at the flowering stage and ensuring high vigor and sufficient pollen supply are important prerequisites and guarantees for obtaining high-quality tobacco seeds and increasing seed yield. The traditional tobacco male sterile hybrid first-generation seed production technology uses fresh male parent pollen to directly pollinate the female parent. The pollen has high vigor and good effect. However, if the female parent has a short growth period and early flowering, while the male parent has a long growth period and flowering If it is late, it is easy to cause the female parent to be fu...
Claims
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