Ultralow temperature preservation method for capsicum pollens

A technology of ultra-low temperature preservation and pollen, which is applied in the direction of plant preservation, botanical equipment and methods, applications, etc. It can solve the problems of flowering failure and achieve the effect of protection loss

Inactive Publication Date: 2012-04-18
HUNAN VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome a series of problems such as parental flowering failure, excessive or lack of pollen, and easy loss of parents in the process of pepper hybrid seed production, the present invention provides a set of safe pepper flower preservation methods, and the preserved pollen can be used at any time , which can completely solve the problem of missing flowering in the production of pepper hybrids. The male parent can be planted in a more secluded place half a year in advance or earlier, so that the male parent of the new variety is not easy to lose, and the new variety can be protected.

Method used

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  • Ultralow temperature preservation method for capsicum pollens
  • Ultralow temperature preservation method for capsicum pollens
  • Ultralow temperature preservation method for capsicum pollens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Relationship between different pretreatment, thawing methods and pollen vigor

[0010] When the conditions are convenient, it is better to pretreat the pollen at -20°C before storage, or directly put the freezing tube containing pepper pollen into liquid nitrogen without pretreatment; Significant difference, so choose to thaw directly indoors.

[0011] Table 1: The relationship between different pretreatments, different thawing methods and pollen viability of pollen stored in liquid nitrogen for one month (experimental time: 2009.2.18)

[0012]

Embodiment 2

[0014] Table 2: The relationship between different pretreatments, different thawing methods and pollen viability of pollen stored in liquid nitrogen for two months (experimental time: 2009.3.23)

[0015]

[0016] Note: In Table 1 and Table 2, the TTC staining method was used to measure the pollen viability, and three repeated slices were made for each treatment, and 5 fields of view were observed for each slice, and the average result was calculated

Embodiment 3

[0018] Table 3 The relationship between pollen stored in liquid nitrogen for half a year in different pretreatments, different thawing methods and pollen viability (experimental time: 2009.6.)

[0019]

[0020] Explanation: A: Represents pre-cooling at 4°C; B: Represents pre-cooling at -20°C; C: Represents direct input into liquid nitrogen at -196°C; due to high temperature in 2009, the pollination rate was low; the above table 1 , 2, 3 The stored pollen was collected in Sanya, Hainan on January 15, 2009, and arrived in Changsha on January 17. It was put into a 2.0ml test tube on January 18, and put into liquid nitrogen after different treatments; 2009.6.16- On June 19, pollen was thawed at -20°C for 30 minutes, and on June 20-6.30, the pollen was thawed directly from liquid nitrogen and thawed naturally. Therefore, it is better to pre-cool at -20°C or put in directly when putting in. For instant noodles, choose to put in directly, and use natural thawing method when thawin...

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Abstract

The invention provides a preservation method for paternal pollens in the capsicum hybrid production. In the capsicum hybrid production, male parents and female parents are planted in a time staggering and allopatry mode, and the pollens of the male parents, which are concentratedly stored in liquid nitrogen, are transported to female parent planting sites for the usage during pollination. According to the invention, the flowering asynchronism of the male parents and the female parents in the capsicum hybrid production is thoroughly solved, so the simultaneous and sympatric appearance of capsicum at the seed production male parents and the seed production female parents is avoided, and the loss of the parent of the male parents can be effectively prevented; and superfluous pollens in the seed production can be stored, so the stored pollens can be used to cover the shortage when the amount of the pollens of the male parents is not enough.

Description

Technical field [0001] The invention relates to a method for preserving pepper pollen, which relates to commercial seed production of pepper, in particular to an ultra-low temperature preservation method for pepper pollen and its application in commercial seed production. Background technique [0002] During the seed production process of pepper hybrids, it often occurs that the parents do not meet at the flowering period, and the excessive pollen of the male parent causes unnecessary waste, and is easily affected by natural factors, which leads to the lack of pollen and affects the yield of seeds. The varieties carefully selected for many years are in the When producing seeds, the parents are planted together and are easy to be stolen. These problems have been plaguing breeders and seed companies all over the world. If the male parents are stored in the form of pollen for a long time and safely, they can be provided to the seed producers at any time, which can completely so...

Claims

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Application Information

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IPC IPC(8): A01N3/00
Inventor 白占兵李雪峰戴雄泽马艳青陈文超张竹青周书栋邹学校
Owner HUNAN VEGETABLE RES INST
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