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Application of epothilone B in preparing medicine for preventing and treating inflammatory bone loss

An epothilone, inflammatory technology, applied in the field of application of epothilone B in the preparation of drugs for the prevention and treatment of inflammatory bone loss

Inactive Publication Date: 2020-04-10
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no related reports that epothilone B regulates the process of osteoclast differentiation and fusion, reduces the release of inflammatory factors in the process of osteoclast differentiation, and achieves the effect of protecting inflammatory bone loss

Method used

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  • Application of epothilone B in preparing medicine for preventing and treating inflammatory bone loss
  • Application of epothilone B in preparing medicine for preventing and treating inflammatory bone loss
  • Application of epothilone B in preparing medicine for preventing and treating inflammatory bone loss

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Embodiment Construction

[0026] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0027] RAW264.7 (mouse mononuclear macrophage cell line) and BMM (mouse primary mononuclear macrophage), in the mouse source RNAKL recombinant protein and M-CSF recombinant protein purchased from the US RD company or the US sigma The company's LPS stimulation can differentiate into osteoclasts, and it is a commonly used cell model for studying biological behaviors such as osteoclast differentiation, fusion, maturation, and osteophagy. At the same time, osteoclasts are closely related and coupled with osteoblasts, bone cells, and stromal cells, and are important regulatory elements for bone matrix dynamic balance and electrolyte homeostasis in vivo. Inflammatory bone loss is often caused by excessive bone resorption caused by excessive release of inflammatory factors, which is the result of excessive activation of osteoclasts. Therefore, RAW2...

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Abstract

The invention relates to an application of epothilone B in preparing a medicine for preventing and treating inflammatory bone loss. In effective dosages, epothilone B has no toxic effect on mononuclear macrophages; and the epothilone B promotes apoptosis of RANKL-induced mononuclear macrophages, strongly inhibits differentiation and maturation of RANKL and LPS induced RAW264.7 and mouse primary mononuclear macrophages to osteoclasts, strongly inhibits bone phagocytosis of osteoclasts obtained by differentiation and maturation of the RANKL and LPS induced RAW264.7 and mouse primary mononuclearmacrophages, inhibits nuclear transformation of NF-kappaB p65 generated by RANKL stimulation, obviously reduces expression of differentiation specific genes of osteoclasts such as NFATc1, c-Fos and the like, obviously inhibits release of nitric oxide in an osteoclasts differentiation process, and strongly inhibits signal paths of stat3, PI3K / Akt and NF-kappaB in the osteoclasts differentiation process.

Description

technical field [0001] The invention belongs to the technical field of orthopedic biological control, and relates to the application of epothilone B in the preparation of medicines for preventing and treating inflammatory bone loss. Background technique [0002] Bone destruction caused by trauma, infection and other reasons is a major problem in the field of orthopedic treatment. Autologous cancellous bone grafting has long been regarded as the gold standard for the treatment of bone defects. In recent years, with the continuous development of biomedical engineering technology, the application of combining tissue-engineered bone with osteogenic seed cells-mesenchymal stem cells and composite materials has gradually been promoted in scientific research and clinical practice. In addition, some inflammation-related bone destruction often manifests as excessive bone resorption and insufficient osteogenesis. Therefore, effective control of bone resorption under inflammatory con...

Claims

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Application Information

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IPC IPC(8): A61K31/427A61P19/08A61P29/00
CPCA61K31/427A61P19/08A61P29/00
Inventor 陈玥琦董世武许建中罗飞王一然
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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