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Optical soybean receptor protein GmZTL2 and application thereof

A photoreceptor and soybean technology, applied in the field of plant genetic engineering, can solve the problems of long cycle, strong parental dependence, and wide adaptability of soybean photoperiod, and achieve the effect of prolonging plant growth period and inhibiting plant flowering

Inactive Publication Date: 2012-10-10
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, the solution to soybean photoperiod sensitivity was mainly to obtain new varieties through hybridization. However, this breeding method has the disadvantages of strong dependence on the parents and long cycle. So far, no soybean varieties with wide adaptability to photoperiod have been obtained.

Method used

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  • Optical soybean receptor protein GmZTL2 and application thereof
  • Optical soybean receptor protein GmZTL2 and application thereof
  • Optical soybean receptor protein GmZTL2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Cloning of soybean photoreceptor gene GmZTL2

[0031] Using the forward primer 5′-ATGGAGTGGGACAGCAATTCCGATC-3′ and the reverse primer 5′-GCAATGCTTAGCATCCATTCCCG-3′, clone and sequence the cDNA of soybean Kennong 18 (purchased from Heilongjiang Bayi Agricultural University) (Glycine max L Kennong 18) The GmZTL2 gene is obtained, the gene sequence of which is shown in SEQ ID NO.2; the amino acid sequence of the protein encoded by it is shown in SEQ ID NO.1.

[0032] The PCR reaction program used to amplify the target gene GmZTL2 was: 95°C 5min pre-denaturation; 95°C 30s, 50°C 35s, 72°C 2min, 25 cycles, 72°C 10min extension.

Embodiment 2

[0033] Example 2 Amino Acid Sequence Analysis of Protein Encoded by Soybean Photoreceptor Gene GmZTL2

[0034] The homology between the GmZTL2 protein sequence of the soybean photoreceptor gene and the amino acid sequence of the Arabidopsis ZTL protein is 86.8%, and the homology is even more high in the conserved functional domains (LOV, F-box and Kelch repeats domain). Therefore, it is speculated that GmZTL2 also has the function of inhibiting flowering similar to the function of the Arabidopsis ZTL gene.

Embodiment 3

[0035] Example 3 Cloning vector of soybean photoreceptor gene GmZTL2

[0036] The PCR product that amplifies and obtains from embodiment 1 is cloned directly according to the TA cloning method image 3 on the indicated pGWCm vector. Firstly, the pGWCm vector was hydrolyzed with Ahd I endonuclease, and then the digested product was recovered with a gel recovery kit to obtain the T vector. Then the PCR product and the T vector were ligated at 16° C. for 12 hours, the ligated product was transformed into Escherichia coli DH5α, amplified therein, positive clones were screened and sequenced.

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PUM

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Abstract

The invention belongs to the field of gene engineering, and provides an optical soybean receptor protein GmZTL2 and application of the optical soybean receptor protein GmZTL2. The protein has an amino acid sequence shown as SEQ ID No.1. The invention also provides a gene for coding the protein. The gene has a nucleotide sequence shown as SEQ ID No.2. By expressing the optical soybean receptor protein GmZTL2, flowering of a plant (arabidopsis thaliana) can be obviously inhibited, the flowering time is delayed, and the growth period is prolonged; and the optical soybean receptor protein GmZTL2 can be used for solving the problems of flowering asynchronism in cross breeding, growth period control of various crops, vegetables, fruits and flowers, photoperiod sensitivity and introduction.

Description

technical field [0001] The invention relates to the field of plant genetic engineering, in particular to a soybean photoreceptor protein, its encoding gene and its application in the regulation of plant photoperiod and flowering time. Background technique [0002] Soybean is one of the important crops, and it is an important source of plant protein, edible oil, biodiesel, and secondary metabolites such as isoflavones and lecithin. Because soybean is a short-day plant, flowering is strictly controlled by photoperiod, so excellent varieties in different regions cannot be introduced to each other, and the growth period is also restricted by environmental photoperiod (Zhang et al., 2008). If the sensitivity of soybean to photoperiod can be reduced, and the limitation of photoperiod on soybean flowering can be broken, the problem of soybean introduction can be solved, so as to realize the exchange of high-quality varieties between regions, enrich excellent germplasm resources in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 傅永福张晓玫
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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