Tulip pollen collection, identification and preservation method and tulip crossbreeding method

A preservation method and pollen collection technology, which are applied in the fields of plant preservation, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of identification of germination rate, influence of pollen quality, long time required for identification of germination rate, etc.

Pending Publication Date: 2019-12-13
台州市农业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technical solution solves some problems of pollen collection and storage in the process of tulip hybrid breeding to a certain extent, but there are still some problems, such as pollen collection selects flowers that are in bud and the pollen is not scattered. Is the pollen vitality right now? The maximum needs to be verified. The pollen collected by default is qualified, and the germination rate is not identified before collection and storage. However, in the prior art, it takes a long time to identify the germination rate of tulip pollen, and the pollen may be affected during this process. quality; and the pollen of this technical solution can be stored for up to 30 days, in some cases, this storage time is still shorter

Method used

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  • Tulip pollen collection, identification and preservation method and tulip crossbreeding method
  • Tulip pollen collection, identification and preservation method and tulip crossbreeding method
  • Tulip pollen collection, identification and preservation method and tulip crossbreeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for identifying tulip pollen vigor, carried out as follows:

[0036] Pollen collection method: bagging the day before the flowers open ( figure 1 ), to prevent biological or mechanical confounding. After 10 a.m. on sunny days (the pollen collected at this time has low humidity, can be directly used for hybrid breeding and preservation without drying, and is easy to preserve), choose flowers that have just cracked petals and completely dispersed pollen ( figure 2 ) (at this time, the pollen germination rate is the highest, the germination speed is the fastest, and the vitality is the largest), use tweezers to collect pollen and put it into a 2ml centrifuge tube, and mark the collection species and time on the centrifuge tube.

[0037] Pollen drying treatment method: pollen collected after 10:00 am on sunny days can be stored directly without drying; pollen collected on sunny days but not completely scattered can be placed in a ventilated place in the greenhous...

Embodiment 2

[0046] Effect of pollen collection time on pollen germination.

[0047] The purpose of the experiment: to determine the best time for pollen collection by identifying the size of pollen vigor in different states of anthers.

[0048] Varieties tested: tulip varieties Lion King and Dow Jones.

[0049] Experimental treatment: 6 treatments were established according to the degree of flower opening and pollen maturity: flower open - 1d (petals not opened, pollen not scattered), 1d (slightly open, half scattered), 2d (fully open, fully scattered), 5d, 8d, 12d (petals withered, pollen dried). Select 6 plants with flowers about to bloom for each tulip variety, and select a stamen from each flower to collect pollen from the -1d day when the flowers open. After the pollen is collected, put it into a 2ml centrifuge tube, and then store it in a -20°C refrigerator for later use. On the 12th day, the pollen germination activity was uniformly identified.

[0050] Identification method: Th...

Embodiment 3

[0055] The effect of light on pollen germination.

[0056] Test method: collect freshly scattered Lion King pollen and Dow Jones pollen in the morning on a sunny day.

[0057] Test treatment: select MS medium + 100g / L sucrose + 6g / L agar powder + 25mg / L boric acid, and set two culture conditions of light (4000lux) and dark (0lux) in the test. Except for the light conditions during cultivation, pollen collection and pollen viability identification were all in accordance with the pollen collection method and 3-hour rapid identification method in Example 1. The pollen germination was observed under a stereomicroscope, and the pollen germination rate and germination speed were calculated. The calculation method was the same as the vigor calculation method in Example 1.

[0058] Test results: The germination rate of pollen under light conditions is high and the germination rate of pollen tubes is significantly faster than that under dark conditions ( Figure 9 ).

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Abstract

The invention discloses a tulip pollen collection, identification and preservation method and a tulip crossbreeding method. The tulip pollen collection, identification and preservation method comprises the following steps that (1), pollen collection is conducted; (2) vigour identification is conducted on collected pollen samples; (3), pollen with the germination rate greater than 5% and the germination speed greater than 5 is selected and preserved, wherein the storage temperature is -20-5 DEG C. According to the tulip pollen collection, identification and preservation method, by means of rapid vigour identification, the pollen good in vigour is preserved, preservation is conducted at low temperature, the preservation time is long, the pollen can maintain the vigour for a long time, the problem of blooming of flowers at different times for tulip crossbreeding is effectively solved, and the scientific basis is provided for parent selection.

Description

technical field [0001] The invention relates to the technical field of pollen collection and preservation, in particular to a method for collection, identification and preservation of tulip pollen and a method for hybrid breeding of tulips. Background technique [0002] Tulip (Tulipa gesneriana L.) is a perennial herb of the genus Tulip in the family Liliaceae with bulbs. Tulips are native to the Mediterranean coast, Central Asia, Turkey and other places. Due to the Mediterranean climate, tulips have the characteristics of adapting to cold and humid winter and hot and dry summer. They have the characteristics of dormancy in summer, rooting and sprouting in autumn and winter but not unearthed. Stretch and grow to form stems and leaves, and bloom in March to April. The optimum temperature for growth and flowering is 15-20°C. The flower bud differentiation is carried out during the storage period when the bulbs are dug up from the pot and placed in a cool outdoor for summer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01N3/00C12Q1/02
CPCA01H1/02A01N3/00G01N33/5097
Inventor 刘君黑银秀郭方其余山红唐兴国米敏
Owner 台州市农业科学研究院
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