Soybean GmFTL4 protein and GmFTL6 protein, and application thereof

A soybean, protein technology, applied in the field of genetic engineering, can solve problems such as changing plant sensitivity and flowering time

Active Publication Date: 2011-09-28
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Changing plant sensitivity to photoperiod and flowering time by mod

Method used

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  • Soybean GmFTL4 protein and GmFTL6 protein, and application thereof
  • Soybean GmFTL4 protein and GmFTL6 protein, and application thereof
  • Soybean GmFTL4 protein and GmFTL6 protein, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Cloning of soybean flowering genes GmFTL4 and GmFTL6

[0028] Using forward primer 5'-ATGGCACGGGAGAACCCCTCT-3' and reverse primer 5'-TTAATATCTCCTTCCACCGCAAC-3' and forward primer 5'-AACACAAACAATATAGTATCTAACA-3' and reverse primer 5'-AAAAAATCTTCATTGAACTTG-3' from soybean Kennong 18 (Glycine max L.Kennong 18) was cloned and sequenced to obtain GmFTL4 and GmFTL6 genes, the gene sequences of which are shown in SEQ ID NO.3 and SEQ ID NO.4; the amino acid sequences of proteins encoded by them are shown in SEQ ID NO.1 and Shown in SEQ ID NO.2.

[0029] The PCR reaction program was: 95°C for 5min pre-denaturation, 95°C for 30S, 45°C for 35S, 72°C for 1min, 25 cycles, and 72°C for 10min extension.

Embodiment 2

[0030] Example 2 Amino Acid Sequence Analysis of Proteins Encoded by Soybean Flowering Genes GmFTL4 and GmFTL6

[0031] The homology between the GmFTL4 and GmFTL6 protein sequences of the soybean flowering gene is greater than 96.5%, and the C-terminal functional domain has a high similarity with Arabidopsis, but there are important differences, such as figure 1 shown. The 131st amino acid residue of soybean GmFTL4 and GmFTL6 protein is aspartic acid (D), the 134th amino acid residue is isoleucine (I), and the 135th amino acid residue is threonine (T). The 137th amino acid residue glutamic acid is (E), the 140th amino acid residue is histidine (H), the 151st amino acid residue asparagine is (N), and the corresponding parts of Arabidopsis are Glutamine (Q), Tyrosine (Y), Alanine (A), Glycine (G), Glutamine (Q) and Tyrosine (Y). These differences lead to weaker activities of GmFTL4 and GmFTL6 than Arabidopsis FT.

Embodiment 3

[0032] The cloning vector of embodiment 3 soybean flowering genes GmFTL4 and GmFTL6

[0033]The PCR product that amplifies and obtains from embodiment 1 is cloned directly according to the TA cloning method image 3 on the indicated pGWCm vector. Firstly, the pGWCm vector was hydrolyzed with Ahd I endonuclease, and then the digested product was recovered with a gel recovery kit to obtain the T vector. Then the PCR product and the T vector were ligated at 16° C., the ligated product was transformed into Escherichia coli DH5α, amplified therein, positive clones were screened and sequenced.

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Abstract

The invention discloses a soybean GmFTL4 protein and a soybean GmFTL6 protein, with an amino acid sequence shown as SEQ ID No.1 and SEQ ID No.2 respectively, or an equally functional amino acid sequence formed through replacement, deletion, or addition of one or a plurality of amino acids of SEQ ID No.1 or SEQ ID No.2. The expression of soybean anthesis genes GmFTL4 and GmFTL6 can obviously promote a plant (Arabidopis thaliana) to blossom, and shorten an anthesis time and a fertility stage. In addition, the invention can be applied to solve problems of flowering asynchronism in crossbreeding,fertility stage control of various crops, vegetables, fruits and flowers, photoperiod sensitivity, and introduction.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to two soybean flowering genes, their encoded proteins and their application in the regulation of plant photoperiod and flowering time. Background technique [0002] Soybean is one of the important crops, and it is an important source of plant protein, edible oil, biodiesel, and secondary metabolites such as isoflavones and lecithin. Because soybean is a short-day plant, flowering is strictly controlled by photoperiod, so excellent varieties in different regions cannot be introduced to each other, and the growth period is also restricted by environmental photoperiod (Zhang et al., 2008). If the sensitivity of soybean to photoperiod can be reduced, and the limitation of soybean flowering to photoperiod can be broken, the problem of soybean introduction can be solved, so as to realize the mutual exchange of high-quality varieties in various regions, enrich excellent germplasm resour...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 傅永福张晓玫
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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